A novel hybrid biomaterial for tissue engineeringhas been developed by forming collagen microsponges in the pores of poly(alpha ester) sponge. Poly(alpha sponge provides skeleton with a high degree mechanical strength, while cell compatibility. The Figure shows mouse fibroblast cells and bovine articular chondrocytes adhered to PLGA–collagen

A novel decellularization method using sonication treatment is described. Sonication the combination of physical and chemical agents. These methods will disrupt cell membrane release contents to external environments. The removal was facilitated by subsequent rinsing sodium dodecyl sulfate detergents. used in preparation complete decellularized bioscaffolds. aim this study confirm usefulness for biological scaffolds. In study, samples aortic tissues are at frequency 170 kHz 0.1% 2%...

A polymer surface with excellent blood compatibility has been produced via spontaneous segregation of a block copolymer. The water-soluble (PME3MA) spontaneously segregates into hydrophobic and hydrophilic environments (see Figure), causing the blocks to expel biological substances including proteins, cells platelets flexible chain motion.

Scaffolds play a key role in the process of regeneration and morphogenesis tissue or organ. We have developed novel sonication decellularization system to prepare decellularized bio-scaffolds short treatment time. The aim study is investigate condition that completely decellularize meniscus can be changed as well maintain biomechanical parameters scaffolds. samples were using treatment. treated evaluated histologically by EVG for cell removal, picrosirius red content collagen type I III,...

(miRNAs) inhibit bone formation by inhibiting the translation of specific genes. Several in vitro studies have suggested that miR-23a inhibits osteogenic differentiation suppressing Runx2, a transcription factor essential for osteoblastogenesis, and Satb2, member special AT rich binding protein family. In present study, we used gain-of-function approach to determine roles homeostasis vivo. The transgenic (Tg) mice grew normally their body size weight were similar those wild-type (WT)...

The absorption and photoluminescence (PL) spectra of negatively photodoped ZnO nanocrystals dispersed in ethanol are measured under a stepwise photodoping process at room temperature. almost simultaneously each level compared detail to discuss the origin spectral change. redshift PL spectrum increase its intensity clearly observed early stage attributed trion luminescence. is found split into two peaks for highly nanocrystals. cause splitting discussed terms dependence on nanocrystal size.

Implantation of sheet-like liver tissues is a promising method in hepatocyte-based therapies, because angiogenesis expected to occur upon implantation from the surrounding tissues. In this context, we introduce here new methodology for formation functional thick hepatic tissue usable cell sheet technology. First, report composite elements suspension culture. Composite were composed human hepatoma Hep G2 cells and mouse NIH/3T3 fibroblasts which are important modulators thick-tissue...

α-TCP/HAp functionally graded porous beads were prepared for use as a scaffold bone regeneration. In this research, which have compositionally layer, from TCP of inner area to HAp surface area, fabricated. It is known that the dissolution rate fast in compare with living body, thus would dissolve prior area. These allow penetration cells and blood vessel into following new At present, conditions fabricate been established already, experiments using [MC3T3-E1] are progress.

Decellularization is a process of tissue treatment targeting cell removal. Sonication system was developed in order to decellularize meniscus tissues. The samples were sonicated 0.1% sodium dodecyl sulphate (SDS) for 10 hours and at 40 kHz ultrasound frequency. All the structurally examined using van Gieson, Picrosirius red, Safranin-O/Fast green staining, scanning electron microscopic (SEM) observation. Histological analysis sonication treated-samples by Gieson staining demonstrated...

We developed an apparatus, which has a structure based on cone and plate-type rheometer, to facilitate quantitative analysis of the detachment process endothelial cells (EC) from diverse materials including 3-dimensional scaffolds such as plate, membrane porous-shaped materials. As artificial vascular model, material inoculated with EC polycarbonate coated laminin was prepared. In consequence, reduced cell number, medium volume, size enough evaluate various by shear stress in our system....

Capillary endothelial cells (ECs) were cultured on gold electrodes in polydimethylsiloxane (PDMS)/glass microdevices. Our objective was to monitor the culture growth by electrical impedance spectroscopy (EIS) measurements, and observe whether formation of capillary-like structures cell layer produces a change spectra. In our contribution, we present novel micro-device, which enables long-term monitoring properties. We illustrate usefulness EIS measurements observing several conditions...

Sonication treatment is used in the preparation of bioscaffolds that was able to support repopulation vascular smooth muscle cells (VSMCs) upon cell-seeding. The aim this study investigate ability sonicatedly decellularized tissue repopulate VSMCs after 6 days In study, sample aorta tissues are by sonication 0.1% and 2% sodium dodecyl sulfate (SDS) detergent for 10 hours. It followed washing process with PBS solution 5 days. Decellularized then cell seeded static seeding 96-well plate...

Recently, regenerative medicine attracts very wide attention. Regenerative is a method of medical care for the purpose recovery lost human organic function, resulted by an accident or diseases. It considered that there are three important factors in medicine, cell, scaffold and cell growth factor. In this research, talyor made stereolithography was used to fabricate highly precise biodegradable which suited individual bone defect part. The sterolithograph, one rapid prototyping methods,...

Although rapid formation of smooth inner surface is important to construct an artificial vascular graft, a conventional model by using biodegradable polymer such as poly-glycolic acid, needs long-term culture form it. In another collagen gel, it reported that prompt the could be realized. However, mechanical property was not suitable, resulting in rupture under high-pressure condition arterial level. Therefore, we propose new graft constituted polymer, and cells. At first, manufactured...

Porous beads of bioactive ceramics such as HAp, TCP are considered to be promising excellent scaffolds for cultivating bone cells. To realize this type which maintains the function scaffold with sufficient strength up growth new bone, and is expected absorbed completely after growth, a-TCP/ HAp functionally graded porous were fabricated. material has both high better tissue-adhesive properties, but that not readily by human body. On contrary, a-TCP highly bioabsorbable; it quickly body, and,...