A5028880273- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Microwave Engineering and Waveguides
- Polyamine Metabolism and Applications
- Renin-Angiotensin System Studies
- Gyrotron and Vacuum Electronics Research
- Enzyme Structure and Function
- Receptor Mechanisms and Signaling
- Hormonal Regulation and Hypertension
- Radio Frequency Integrated Circuit Design
- Neuropeptides and Animal Physiology
- Oral microbiology and periodontitis research
- Epigenetics and DNA Methylation
- Eicosanoids and Hypertension Pharmacology
- Neuroscience and Neuropharmacology Research
- Heart Failure Treatment and Management
- Nitric Oxide and Endothelin Effects
- Cancer, Hypoxia, and Metabolism
- Streptococcal Infections and Treatments
- Tryptophan and brain disorders
- Chemical Synthesis and Analysis
- Microstructure and Mechanical Properties of Steels
- Organophosphorus compounds synthesis
- Chemical Synthesis and Reactions
- Energy Efficient Wireless Sensor Networks
Tokushima University
2012-2021
Amano Enzyme (Japan)
2004-2017
Japan Science and Technology Agency
2010-2015
University Medical Center of the Johannes Gutenberg University Mainz
2011
Johannes Gutenberg University Mainz
2011
Oki Electric Industry (Japan)
2006-2009
Cosmos Corporation (United States)
2006
Okayama University
1990-2005
University of Wisconsin–Madison
2005
Toyohashi Municipal Hospital
2004
A continuous cell line (YT cells) with inducible receptor for T growth factor (TCGF)/interleukin 2 (IL 2) was established from a 15-yr-old boy acute lymphoblastic lymphoma and thymoma. YT cells were tetraploid, having 4q+ chromosomal markers, proliferated continuously in vitro without conditioned medium (CM) or IL 2. They weakly positive OKT9, OKT11, Tac antigen (Ag), determinant closely associated the 2-R), negative OKT1, OKT3, OKT4, OKT8 Ag. also expressed HNK-1 Ag Fc receptors IgG, which...
The gene encoding glucosyltransferase responsible for water-insoluble glucan synthesis (GTF-I) of Streptococcus sobrinus (formerly mutans 6715) was cloned, expressed, and sequenced. A bank from S. 6715 DNA constructed in vector pUC18 screened with anti-GTF-I antibody to detect clones producing GTF-I peptide. Five immunopositive were isolated, all which produced peptides that bound alpha-1,6 glucan. activity found only two large peptides: one stretching over the full length peptide composed...
d ‐Amino acid oxidase (DAAO) has been proposed to be involved in the oxidation of ‐serine, an allosteric activator NMDA‐type glutamate receptor brain, and associated with onset schizophrenia. The recombinant human DAAO was expressed Escherichia coli isolated as active homodimeric flavoenzyme. It shows properties dehydrogenase‐oxidase class flavoproteins, possesses a low kinetic efficiency, follows ternary complex (sequential) mechanism. In contrast other known DAAOs, enzyme is stable...
Human interleukin 2 receptors ( IL2R ) from various cell sources such as mitogen-activated normal human T cells, adult leukemia (ATL)-derived line cells (MT-1, ATL-6, Hut-102), and a natural killer-like YT were studied both by one- two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with monoclonal anti- antibody (anti-Tac). In synthetic labeling [35S]methionine, anti-Tac specifically precipitated two glycoproteins, one m.w. of 60,000 to 65,000 pI 4.2 4.7,...
In the brain, extensively studied FAD-dependent enzyme D-amino acid oxidase (DAO) degrades gliotransmitter D-serine, a potent activator of N-methyl-D-aspartate type glutamate receptors, and evidence suggests that DAO, together with its G72 protein, may play key role in pathophysiology schizophrenia. Indeed, potential clinical importance highlights need for structural functional analyses human DAO. We recently succeeded purifying found it weakly binds FAD shows significant slower rate flavin...
Summary The amount of immunoreactive thromboxane B2 (TXB2) released from isolated canine arteries was determined by radioimmunoassay. TXB2 the cerebral, coronary, mesenteric, and saphenous 47.0 ± 7.2, 4.0 0.6, 4.9 0.5, 2.7 0.4 pg/mg wet weight tissue/30 min, respectively. release cerebral artery decreased to <5% administration indomethacin (10-5M) or OKY-046 (10-4M), intimal rubbing. enhanced nearly twofold addition arachidonic acid (AA) medium, but not acetylcholine (ACh) (10-6M). arterial...
cDNA clones encoding D‐amino acid oxidase were isolated from a human kidney library by hybridization with for the pig enzyme. The insert of 2.0 kilobase pairs long provided coding information protein consisting 347 amino acids. molecular mass enzyme was calculated to be 39 410 Da. sequence similarity between and enzymes is 84.4%, among active site residues proposed chemical modification studies, methionine‐110 replaced threonine. Northern blot analysis confirmed expression an mRNA kilobases...
Complementary DNAs encoding D-amino acid oxidase (EC 1.4.3.3, DAO), one of the principal and characteristic enzymes peroxisomes porcine kidney, have been isolated from kidney cDNA library by hybridization with synthetic oligonucleotide probes corresponding to partial amino sequences. Analysis nucleotide sequences two clones revealed a complete 3211-nucleotide sequence 5'-terminal untranslated region 198 nucleotides, 1041 nucleotides an open reading frame that encoded 347 acids, 3'-terminal...
A glucosyltransferase responsible for water-insoluble glucan synthesis was purified from the culture fluids of Streptococcus mutans 6715-15 strain by column chromatography on Toyopearl HW-60 and subsequently hydroxyapatite. The enzyme preparation gave a single band analysis polyacrylamide gel electrophoresis. pH dependency activity showed two optimal peaks at 5.8 7.3 Km values sucrose were 1.4 3.3 mM respective pHs. molecular weight determined sodium dodecyl sulfate electrophoresis 180,000....
Nucling is a novel apoptosis-associated molecule, which involved with cytochrome c/Apaf-1/caspase-9 apoptosome induction following pro-apoptotic stress. In the present study, we show first that able to interact galectin-3. Galectin-3 known participate in many biological processes, including apoptotic cell death. was found down-regulate expression level of galectin-3 mRNA/protein. Nucling-deficient cells, up-regulated, appeared be resistant some forms stress as compared wild-type cells....
Three colonial variants of Actinobacillus actinomycetemcomitans, which formed transparent rough (TR)-, smooth (TS)-, and opaque (OS)-surfaced colonies, were described in relation to their fimbriation. TR- TS-cells adhesive agar glass surfaces but not the OS-cells. The examination by electron microscopy revealed that TR-cells highly fimbriated TS- Thus, seemed be an intermediate type. fimbriae isolated from suspending 0.15 M ethanolamine-HCl buffer (pH 10.5) purified dissolving non-fimbrial...
Six new triterpenoid glycosides called julibrosides A1-A4, B1 and C1 were isolated from Albizziae Cortex, the dried stem bark of Albizzia julibrissin DURAZZ. Their structures determined based on spectral chemical evidence. Julibrosides had sapogenols, designated julibrogenin B C, respectively, while A3 included N-acetyl-D-glucosamine as a sugar component.
Expression conditions inEscherichia coli of wild-type, Y224F, and Y228F mutants pig kidney D-amino acid oxidase (DAAO) have been changed to yield more enzyme.The mutated proteins show spectral properties similar those the wild-type enzyme, in all oxidationreduction states.All enzymes were studied by steady state rapid reaction methods.Turnover numbers determined for Y224F DAAO with different substrates protein, while always showed lower turnover higher K,,, values acid.Analyses reduction...
The current study was designed to evaluate the role of angiotesin II (ANG II) in regulation renin mRNA and angiotensinogen levels. We investigated changes levels kidney liver induced by ANG infusion inhibition endogenous renin-angiotensin system with enalapril or saralasin rats. mRNAs for were measured densitometric analysis Northern blot hybridization. After a 4-h intravenous (0.2 micrograms.kg-1.min-1), level increased 2.5-fold control level, decreased 45%. Four hours after treatment...