A5069026909- Mesenchymal stem cell research
- Oral microbiology and periodontitis research
- Periodontal Regeneration and Treatments
- Oral Health Pathology and Treatment
- Cancer-related molecular mechanisms research
- Retinal Diseases and Treatments
- Oral and Maxillofacial Pathology
- Immune Response and Inflammation
- Genomics and Phylogenetic Studies
- dental development and anomalies
- Bone and Dental Protein Studies
- Retinal and Optic Conditions
- Tissue Engineering and Regenerative Medicine
- Bone health and treatments
- Microbial infections and disease research
- Retinopathy of Prematurity Studies
- Bone Metabolism and Diseases
- Cancer Cells and Metastasis
- Macrophage Migration Inhibitory Factor
- S100 Proteins and Annexins
- Antimicrobial Peptides and Activities
- Proteoglycans and glycosaminoglycans research
- Interpreting and Communication in Healthcare
- HIV/AIDS oral health manifestations
- Dental Trauma and Treatments
Kyushu Dental University
2019-2025
Harvard University
2023-2025
Joslin Diabetes Center
2023-2025
Tokyo Women's Medical University
2016-2019
Tokyo Medical and Dental University
2016-2018
Periodontitis results in the destruction of tooth-supporting periodontal tissues and does not have ability to heal spontaneously. Various approaches been introduced regenerate tissues; however, these limited efficacy for treating severe defects. Cytotherapies combine stem cell biology tissue engineering form a promising approach overcoming limitations. In this study, we isolated ligament (PDL)-derived cells from patients created sheets with "Cell Sheet Engineering Technology", using...
Insulin resistance and hyperglycemia are risk factors for periodontitis poor wound healing in diabetes, which have been associated with selective loss of insulin activation the PI3K/Akt pathway gingiva. This study showed that mouse gingiva due to deletion smooth muscle fibroblast receptor (SMIRKO mice) or systemic metabolic changes induced by a high-fat diet (HFD) HFD-fed mice exacerbated periodontitis-induced alveolar bone loss, preceded delayed neutrophil monocyte recruitment impaired...
<p dir="ltr">Anti-vascular endothelial growth factor (anti-VEGF) therapies are effective treatment of severe diabetic retinopathy (DR) and macular edema, but a significant subset people showed inadequate response to anti-VEGF intervention. Since elevation or overexpressing retinol binding protein 3 (RBP3) decreased risks for retinal pathologies progression DR, we compared the therapeutic profile RBP3 normalize dysfunctions induced by diabetes. Intravitreous injection recombinant human...
<p dir="ltr">Anti-vascular endothelial growth factor (anti-VEGF) therapies are effective treatment of severe diabetic retinopathy (DR) and macular edema, but a significant subset people showed inadequate response to anti-VEGF intervention. Since elevation or overexpressing retinol binding protein 3 (RBP3) decreased risks for retinal pathologies progression DR, we compared the therapeutic profile RBP3 normalize dysfunctions induced by diabetes. Intravitreous injection recombinant human...
Anti-vascular endothelial growth factor (anti-VEGF) therapies are effective treatment of severe diabetic retinopathy (DR) and macular edema, but a significant subset people showed inadequate response to anti-VEGF intervention. Since elevation or overexpressing retinol binding protein 3 (RBP3) decreased risks for retinal pathologies progression DR, we compared the therapeutic profile RBP3 normalize dysfunctions induced by diabetes. Intravitreous injection recombinant human (rhRBP3) antibodies...
Human multipotent mesenchymal stromal cells (hMSCs) possess the ability to differentiate into osteoblasts, and they can be utilized as a source for bone regenerative therapy. Osteoinductive pretreatment, which induces osteoblastic differentiation of hMSCs in vitro, has been widely used tissue engineering prior cell transplantation. However, molecular basis induced by osteoinductive medium (OIM) is still unknown. Therefore, we next-generation sequencer investigate changes gene expression...
Human periodontal ligament mesenchymal stem cells (hPDLMSCs) have been known that they play important roles in homeostasis and regeneration of tissues. Additionally, spheroids are superior to monolayer-cultured cells. We investigated the characteristics potential tissue co-cultured hPDLMSCs human umbilical vein endothelial (HUVECs) vitro vivo. Co-cultured were prepared with cell ratios hPDLMSCs: HUVECs = 1:1, 1:2, 2:1, using microwell chips. Real-time polymerase chain reaction (PCR)...
Periodontitis is an inflammatory disease caused by pathogenic oral microorganisms that induce the destruction of periodontal tissue. We sought to identify relevant differentially expressed genes (DEGs) and clarify mechanism underlying rapid alveolar bone loss using ligature-induced periodontitis in mice. A silk ligature was tied around maxillary left second molar 9-week-old C57BL/6 J male In-vivo micro-CT analysis revealed ligation induced severe loss. RNA-sequencing analysis, examine host...
Microbial contamination poses a major difficulty for successful data analysis in biological and biomedical research. Computational approaches utilizing next-generation sequencing (NGS) offer promising diagnostics to assess the presence of contaminants. However, as host cells are often contaminated by multiple microorganisms, these require careful attention intra- interspecies sequence similarities, which have not yet been fully addressed.We present computational approach that rigorously...
Many cases of bisphosphonate-related osteonecrosis the jaw (BRONJ), which is an intractable disease, have been reported. Although a general intravenous injection multipotent mesenchymal stromal cells (MSCs) may be effective for treating BRONJ, it has some severe problems. Therefore, our aim was to develop treatment locally administered MSCs. In this study, we investigated effect MSC sheet transplantation in mandibular bone healing beagle dogs, were zoledronate and dexamethasone. MSCs...
Abstract Background Multipotent mesenchymal stromal cells (MSCs) can be isolated from numerous tissues and are attractive candidates for therapeutic clinical applications due to their immunomodulatory pro-regenerative capacity. Although the minimum criteria defining MSCs have been defined, characteristics known vary depending on tissue of origin. Results We characterized human three different bones (ilium (I-MSCs), maxilla (Mx-MSCs) mandible (Md-MSCs)) proceeded with next generation...
Large animal experiments are important for translational research in regenerative medicine. Recently, mini pigs have been used large studies and surgical training. The use of multipotent mesenchymal stromal cell (MSC) sheets the treatment many diseases is increasing. purpose present study was to establish optimal methods generating pig MSC from various tissues compare properties MSCs these sheets.MSCs were isolated bone marrow, adipose, periodontal ligament, gingiva, or periosteum pigs....
Studies suggest that analysis of gingival crevicular fluid (GCF) is useful for evaluating periodontal status. In this study, clinical variables related to tooth mobility, and multiple cytokine levels in proximate GCF, were measured at four time points during initial treatment: before treatment (baseline), after supragingival scaling, occlusal adjustment, scaling root planing (SRP); 20 teeth from 13 patients with periodontitis included. Baseline interleukin (IL)-10 level GCF was significantly...
A spheroid is known as a three-dimensional culture model, which better simulates the physiological conditions of stem cells. This study aimed at identifying genes specifically expressed in spheroid-cultured human periodontal ligament mesenchymal cells (hPDLMSCs) using RNA-seq analysis to evaluate their functions. Transcriptome was performed and monolayer cultures hPDLMSCs from four patients. Cluster Gene Ontology analyses revealed that involved cell-cell adhesion well G2/M G1/S transitions...
is the most pathogenic periodontal bacterium in world. Recently,
Abstract Background Multipotent mesenchymal stromal cells (MSCs) can be isolated from numerous tissues and are attractive candidates for therapeutic clinical applications due to their immunomodulatory pro-regenerative capacity. Although the minimum criteria defining MSCs have been defined, characteristics known vary depending on tissue of origin.Results We characterized human three different bones (ilium (I-MSCs), maxilla (Mx-MSCs) mandibular (Md-MSCs)) proceeded with next generation...
<p> </p> <p>Insulin resistance and hyperglycemia are risk factors for periodontitis poor wound healing in diabetes, which have been associated with selective loss of insulin- activation the PI3K/Akt pathway gingiva. This study showed that insulin mouse gingiva due to deletion smooth muscle fibroblast receptor (SMIRKO mice) or systemic metabolic changes induced by high fat diet (HFD) HFD-fed mice exacerbated periodontitis-induced alveolar bone loss, preceded delayed...
<p> </p> <p>Insulin resistance and hyperglycemia are risk factors for periodontitis poor wound healing in diabetes, which have been associated with selective loss of insulin- activation the PI3K/Akt pathway gingiva. This study showed that insulin mouse gingiva due to deletion smooth muscle fibroblast receptor (SMIRKO mice) or systemic metabolic changes induced by high fat diet (HFD) HFD-fed mice exacerbated periodontitis-induced alveolar bone loss, preceded delayed...
Periodontitis is a chronic inflammatory disorder that causes deterioration of the periodontal tissues support teeth. Several biomaterials, such as bone graft materials, barrier membranes and protein products, have been developed used clinically to repair tissue defects clinically. However, it difficult regenerate complete structure. Recently, regenerative therapies using multipotent mesenchymal stromal cells (MSC) introduced overcome limitation conventional procedures. These cytotherapies...
Abstract Background Microbial contamination impedes successful biological and biomedical research. Computational approaches utilizing next-generation sequencing (NGS) data offer promising diagnostics to assess the presence of contaminants. However, as host cells are often contaminated by multiple microorganisms, these require careful attention intra- interspecies sequence similarities, which have not yet been fully addressed. Results We present a computational approach that rigorously...
Background: Multipotent mesenchymal stromal cells (MSCs) can be isolated from numerous tissues and are attractive candidates for therapeutic clinical applications due to their immunomodulatory pro-regenerative capacity. Although the minimum criteria defining MSCs have been defined, characteristics known vary depending on tissue of origin.Methods: We characterized human three different bones (ilium (I-MSCs), maxilla (Mx-MSCs) mandibular (Md-MSCs)) proceeded with next generation...