A5059010005- Prostate Cancer Treatment and Research
- Drug Transport and Resistance Mechanisms
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Estrogen and related hormone effects
- Ubiquitin and proteasome pathways
- Hormonal and reproductive studies
- RNA modifications and cancer
- DNA Repair Mechanisms
- Cancer, Lipids, and Metabolism
- Pharmacological Effects and Toxicity Studies
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Cancer therapeutics and mechanisms
- Metabolism and Genetic Disorders
- ATP Synthase and ATPases Research
- Heat shock proteins research
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Bladder and Urothelial Cancer Treatments
- Amino Acid Enzymes and Metabolism
- Extracellular vesicles in disease
- Ferroptosis and cancer prognosis
- Autophagy in Disease and Therapy
Kyushu University
2016-2025
Department of Medical Sciences
2023-2024
Kyushu University Hospital
2015-2024
University of Occupational and Environmental Health Japan
2002-2016
Jikei University School of Medicine
2016
St Mary's Hospital
2011-2012
St Mary's Hospital
2011
University of British Columbia
2011
McMaster University
2011
Kumamoto University
2002-2010
Mesenchymal stem cell (MSC)–derived exosome plays a central role in the cell-free therapeutics involving MSCs and contents can be customized under disease-associated microenvironments. However, optimal MSC-preconditioning to enhance its therapeutic potential is largely unknown. Here, we show that preconditioning of gingival tissue-derived (GMSCs) with tumor necrosis factor-alpha (TNF-α) ideal for treatment periodontitis. TNF-α stimulation not only increased amount secreted from GMSCs, but...
Members of the ATP-binding cassette (ABC) transporter superfamily are mutated to cause diseases that include cystic fibrosis, hyperinsulinemia, adrenoleukodys-trophy, Stargardt disease and multidrug resistance. We recently isolated a novel human member ABC as candidate for glucuronide glutathione-conjugated antitumor agents, found it highly homologous rat cmoat gene. Consistent with recent findings defects in gene two models hyperbilirubinemia (TR− Eisai), we report deletions missense...
The human multidrug resistance 1 (MDR1) gene encoding P-glycoprotein is often overexpressed in various tumors after chemotherapy. During treatment with chemotherapeutic agents, the MDR1 activated at transcriptional level and/or amplified, resulting overexpression. Our previous studies demonstrated that an inverted CCAAT box (Y-box) might be a critical cis-regulatory element regulating UV or drug-induced expression. We have now established cell lines from head and neck cancer KB cells which...
Mitophagy, which selectively degrades mitochondria via autophagy, has a significant role in mitochondrial quality control. When mitophagy is induced yeast, residential protein Atg32 binds Atg11, an adaptor for selective types of and it recruited into the vacuole along with mitochondria. The Atg11-Atg32 interaction believed to be initial molecular step autophagic machinery recognizes as cargo, although how this mediated poorly understood. Therefore, we studied detail. We found that C-terminus...
The Y-box binding protein, YB-1, is a member of DNA protein family with structurally and functionally conserved cold shock domain. Using Western blotting immunohistochemical methods, larger amounts YB-1 were detected in the cytosol, particularly at perinuclear region, than nucleus human cancer cells. UV irradiation increased accumulation 20 min thereafter. This translocation into by was blocked kinase inhibitor H-7, but not HA-1004. Both green fluorescent (GFP)-YB-1 GFP-YB-1C C-terminus...
p32 is an evolutionarily conserved and ubiquitously expressed multifunctional protein. Although exists at diverse intra extracellular sites, it predominantly localized to the mitochondrial matrix near nucleoid associated with transcription factor A. Nonetheless, its function in poorly understood. Here, we determined via generation of p32-knockout mice. p32-deficient mice exhibited mid-gestation lethality a severe developmental defect embryo. Primary embryonic fibroblasts isolated from...
Some mutations of the DHODH (dihydro-orotate dehydrogenase) gene lead to postaxial acrofacial dysostosis or Miller syndrome. Only is localized at mitochondria among enzymes de novo pyrimidine biosynthesis pathway. Since pathway coupled mitochondrial RC (respiratory chain) via DHODH, impairment should affect function. To investigate this, we used siRNA (small interfering RNA)-mediated knockdown and observed that induced cell growth retardation because G2/M cell-cycle arrest, whereas...
Immunoregulatory properties of mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) are promising. Gingival tissue-derived MSCs (GMSCs) have unique immunoregulatory capacity and secrete large amounts EVs. Recent findings suggest that priming with inflammatory stimuli is an effective strategy for cell-free therapy. However, the precise mechanism by which contents EVs customized has not been fully elucidated. Here, we show derived from GMSCs primed a combination two...
The canalicular multispecific organic anion transporter (cMOAT), also termed MRP2, is a recently identified ATP-binding cassette transporter. We previously established stable human cMOAT cDNA-transfected cells, LLC/cMOAT-1 from LLC-PK1 and LLC/CMV cells that were transfected with an empty vector. found have increased resistance to vincristine (VCR), 7-ethyl-10-hydroxy-camptothecin, cisplatin but not etoposide. multidrug resistance-reversing agents cyclosporin A (CsA)...
We established stable human canalicular multispecific organic anion transporter ( cMOAT/MRP2 ) cDNA transfectants, CHO/cMOAT from non‐polarized Chinese hamster ovary (CHO)‐K1 and LLC/cMOAT polarized pig kidney epithelial LLC‐PK1. Human cMOAT was mainly localized in the plasma membrane of apical LLC/cMOAT. The ATP‐dependent uptake leukotriene C 4 (LTC into vesicles enhanced compared with empty vector transfectants. K m values were 0.24 μM for LTC 175 ATP. Drug sensitivity to vincristine...
We investigated the effects of grapefruit juice (GFJ) and orange (OJ) on drug transport by MDR1 P-glycoprotein (P-gp) multidrug resistance protein 2 (MRP2), which are efflux transporters expressed in human small intestine. examined transcellular uptake [(3)H]vinblastine (VBL) [(14)C]saquinavir a colon carcinoma cell line (Caco-2) porcine kidney epithelial lines transfected with cDNA MRP2 cDNA, LLC-GA5-COL150, LLC-MRP2, respectively. In Caco-2 cells, basal-to-apical transports [(3)H]VBL were...
Plk (polo-like kinase) is a serine-threonine kinase that appears to function in mitotic control mammalian cells. We demonstrated previously PLK mRNA expression low at the G1-S transition, increases during S phase, and maximally expressed G2-M. In present study, we have cloned human gene analyzed structure of 2 kilobases its 5′-flanking region. Using synchronized cultures HeLa cells transfected with promoter/luciferase constructs, show promoter activated phase maximal G2-M phase. various...
Abstract Gene expression can be regulated by nuclear factors at the transcriptional level. Many such regulate MDR1 gene expression, but what are sequence elements and transcription that control basal inducible of this gene? The general principles through which participate in drug resistance now beginning to understood. Here, we review involved regulation gene. In particular, focus on factor Y-box binding protein 1 discuss possible links between cancer, mediated transmembrane P-glycoprotein...