A5052639539- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Spaceflight effects on biology
- Growth Hormone and Insulin-like Growth Factors
- Mitochondrial Function and Pathology
- Bone Tissue Engineering Materials
- Retinal Diseases and Treatments
- TGF-β signaling in diseases
- Bone Metabolism and Diseases
- Redox biology and oxidative stress
- Cancer, Hypoxia, and Metabolism
- Retinal and Optic Conditions
- Retinal Imaging and Analysis
- Sulfur Compounds in Biology
- Metabolism, Diabetes, and Cancer
- Bone health and osteoporosis research
- Bee Products Chemical Analysis
- Tea Polyphenols and Effects
- Genomics, phytochemicals, and oxidative stress
- Human Health and Disease
- Fibroblast Growth Factor Research
- Periodontal Regeneration and Treatments
- Platelet Disorders and Treatments
- Acute Kidney Injury Research
- Dialysis and Renal Disease Management
University of Tsukuba
2023
Hokkaido University
2023
Senshu University
2023
University of Toyama
2023
Tokyo Metropolitan Institute of Gerontology
2003-2016
Tohoku University
2003-2011
Nagara Medical Center
2010
Tokyo Metropolitan Aoyama High School
2006
Kyoto University
2004
Kyushu University
2004
Longevity is regulated by the daf-2 gene network in Caenorhabditis elegans. Mutations gene, which encodes a member of insulin receptor family, confer life extension (Age) phenotype and constitutive dauer (a growth-arrested larval form specialized for dispersal) formation phenotype. The Age mutually potentiated two mutations clk-1 homologue yeast CAT5/COQ7 known to regulate ubiquinone biosynthesis. In this study, we demonstrated that mutation also conferred an oxidative stress resistance...
Trehalose is a disaccharide of glucose found in diverse organisms and suggested to act as stress protectant against heat, cold, desiccation, anoxia, oxidation. Here, we demonstrate that treatment Caenorhabditis elegans with trehalose starting from the young-adult stage extended mean life span by over 30% without any side effects. Surprisingly, even old-adult shortly thereafter retarded age-associated decline survivorship remaining 60%. Demographic analyses age-specific mortality rates...
We investigated the effects of grapefruit juice (GFJ) and orange (OJ) on drug transport by MDR1 P-glycoprotein (P-gp) multidrug resistance protein 2 (MRP2), which are efflux transporters expressed in human small intestine. examined transcellular uptake [(3)H]vinblastine (VBL) [(14)C]saquinavir a colon carcinoma cell line (Caco-2) porcine kidney epithelial lines transfected with cDNA MRP2 cDNA, LLC-GA5-COL150, LLC-MRP2, respectively. In Caco-2 cells, basal-to-apical transports [(3)H]VBL were...
Background One of the most important challenges in study aging is to discover compounds with longevity-promoting activities and unravel their underlying mechanisms. Royal jelly (RJ) has been reported possess diverse beneficial properties. Furthermore, protease-treated RJ (pRJ) additional pharmacological activities. Exactly how pRJ exert these effects which components are responsible for largely unknown. The evolutionarily conserved mechanisms that control longevity have indicated. purpose...
Evidence has been accumulating that in many tumors, insulin-like growth factors (IGFs) promote cancer cell an autocrine/paracrine manner via the IGF-I receptor. In effort to understand role of IGFs prostate growth, we characterized IGF system components produced by human prostatic cell-lines, LNCaP, DU145, and PC-3, grown serum-free medium.IGFs, their receptors, binding proteins (IGFBPs) three lines were reverse transcriptase-polymerase chain reaction (RT-PCR), radioimmunoassay (RIA),...
How microgravitational space environments affect aging is not well understood. We observed that, in Caenorhabditis elegans, spaceflight suppressed the formation of transgenically expressed polyglutamine aggregates, which normally accumulate with increasing age. Moreover, inactivation each seven genes that were down-regulated extended lifespan on ground. These encode proteins are likely related to neuronal or endocrine signaling: acetylcholine receptor, transporter, choline acetyltransferase,...
Royal jelly (RJ) produced by honeybees has been reported to possess diverse health-beneficial properties and implicated have a function in longevity across species as well honeybees. 10-Hydroxy-2-decenoic acid (10-HDA), the major lipid component of RJ honeybees, was previously shown increase lifespan Caenorhabditis elegans. The objective this study is elucidate signaling pathways that are involved extension 10-HDA. 10-HDA further extended daf-2 mutants, which exhibit long through reducing...
Insulin-like growth factor-binding protein-4 (IGFBP-4), like the five other IGFBPs present in human serum, acts as a transport protein for insulin-like factor I (IGF-I) and IGF-II modulates their biological effects. To investigate role of IGFBP-4 physiology IGF system, we developed sensitive RIA employing, antigen, tracer, standard, recombinant (rhIGFBP-4) expressed Escherichia coli fusion with glutathione S-transferase affinity purified glutathione-derivatized resin. Antibody against...
Bone morphogenetic proteins (BMPs) have the unique ability to convert mesenchymal cells into matrix-producing osteoblasts. To understand mechanism(s) by which a BMP produces multitude of effects on bone cells, we examined recombinant human osteogenic protein (OP)-1 (referred as BMP-7) insulin-like growth factor (IGF) regulatory system, an important system in bone. After 48 h treatment, OP-1 increased level IGF-II (3- and 2-fold, respectively, at 100 ng/ml) conditioned medium (CM) SaOS-2 TE85...
Abstract Background Senescence-accelerated mice (SAM) are a series of mouse strains originally derived from unexpected crosses between AKR/J and unknown mice, which phenotypically distinct senescence-prone (SAMP) -resistant (SAMR) inbred were subsequently established. Although SAMP have been widely used for aging research focusing on their short life spans various age-related phenotypes, such as immune dysfunction, osteoporosis, brain atrophy, the responsible gene mutations not yet fully...
The prospect of space travel continues to capture the imagination. Several competing companies are now promising flights for general population. Previously, it was recognized that many physiological changes occur with spaceflight similar those seen normal ageing. This led notion can be used as a model accelerated ageing and raised concerns about safety individuals engaging in travel. Paradoxically, however, has been recently shown beneficial some aspects muscle health tiny worm...
Although the effi cacy of in vivo osteogenic capabilities synthetic octacalcium phosphate (OCP) crystal implantation can be explained through its stimulatory capacity for differentiation host osteoblastic cell lineage, direct evidence that OCP supports bone regeneration by cells has not been shown.Mesenchymal stem (MSCs) isolated from 4-week-old male Wistar rat long bones were pre-incubated or maintenance medium presence absence basic fi broblast growth factor (bFGF).OCP/Collagen (OCP/Col)...
Abstract The insulin/IGF-1 signalling (IIS) pathway plays an important role in the regulation of larval diapause, long-lived growth arrest state called dauer arrest, Caenorhabditis elegans . In this nematode, 40 insulin-like peptides (ILPs) have been identified as putative ligands IIS pathway; however, it remains unknown how ILPs modulate diapause. Here we show that secretory polarity INS-35 and INS-7, which suppress is changed intestinal epithelial cells at These are secreted from intestine...
Extracellular calcium concentration is critically important for normal function of the body. Recently, reports have shown that cells derived from parathyroid glands contain an extracellular receptor responsive to changes in calcium. Bone intimately involved homeostasis; therefore, we sought test hypothesis has direct effects on bone cells. was increased by addition varying concentrations CaCl2 (0.4-2.0 mM) control medium. An increase cell proliferation, as assessed 3H-thymidine...
The nematode Caenorhabditis elegans yields a substance(s) inducing the larval diapause, called dauer-inducing pheromone. We discovered that crude pheromone extract extends adult lifespan in animal. This extension does not occur mutant animal, which expansion of caused by other mutations reducing insulin signaling is suppressed. first description concerning relevancy to longevity