A5072365724- Bone and Dental Protein Studies
- Oral microbiology and periodontitis research
- Bone Metabolism and Diseases
- Periodontal Regeneration and Treatments
- dental development and anomalies
- Oral Health Pathology and Treatment
- HIV/AIDS oral health manifestations
- Salivary Gland Disorders and Functions
- Oral and gingival health research
- Dental Implant Techniques and Outcomes
- Mesenchymal stem cell research
- Cell Adhesion Molecules Research
- Dental Radiography and Imaging
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Oral and Maxillofacial Pathology
- Bone health and treatments
- Streptococcal Infections and Treatments
- MicroRNA in disease regulation
- Viral-associated cancers and disorders
- Dental Health and Care Utilization
- Dental Trauma and Treatments
- Inflammatory mediators and NSAID effects
- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
Nihon University
2016-2025
British Society of Periodontology
2003-2024
Tsurumi University
2012
University of Dental Medicine
2012
Aichi Gakuin University
2012
Asahi University
2012
Health Sciences University of Hokkaido
2012
Osaka University
2012
Meikai University
2003
Niigata University
2003
The efficacy of the local application recombinant human fibroblast growth factor-2 (FGF-2) in periodontal regeneration has been investigated. In this study, a randomized, double-blind, placebo-controlled clinical trial was conducted 253 adult patients with periodontitis. Modified Widman surgery performed, during which 200 µL investigational formulation containing 0% (vehicle alone), 0.2%, 0.3%, or 0.4% FGF-2 administered to 2- 3-walled vertical bone defects. Each dose showed significant...
Abstract Oxidative stress regulates cellular functions in multiple pathological conditions, including bone formation by osteoblastic cells. However, little is known about the mechanisms responsible for effects of oxidative on osteoblast senescence. To clarify inhibitory mineralization, we examined relationship between antioxidant system and MC3T3‐E1 After a single exposure to H2O2 within range non‐toxic concentration cells, mineralization level was diminished half. Under same gene expression...
ABSTRACT We investigated the efficacy, safety, and clinical significance of trafermin, a recombinant human fibroblast growth factor (rhFGF)-2, for periodontal regeneration in intrabony defects Phase III trials. Study A, multicenter, randomized, double-blind, placebo-controlled study, was conducted at 24 centers. Patients with periodontitis 4-mm 3-mm or deeper probing pocket depth defects, respectively, were included. A total 328 patients randomly assigned (2:1) to receive 0.3% rhFGF-2...
Periodontitis, a complex chronic inflammatory disease caused by subgingival infection, is among the most prevalent microbial diseases in humans. Although traditional microbiological research on periodontitis has focused putative bacteria such as Porphyromonas gingivalis, herpes virus proposed to be involved pathogenesis of because bacterial etiology alone does not adequately explain various clinical aspects. In this study, we established for first time, more Epstein–Barr (EBV) DNA found...
Periodontitis is a chronic inflammatory disease caused by specific bacteria and viruses. Local, systemic, environmental factors affect the rate of progression. Immune responses to bacterial products, subsequent production cytokines, are crucial in destruction periodontal tissue. MicroRNAs (miRNAs) class small RNAs that control various cell processes negatively regulating protein-coding genes. In this study, we compared miRNA expression inflamed noninflamed gingival tissues from Japanese...
Chronic periodontitis is a silent infectious disease prevalent worldwide and affects lifestyle-related diseases. Therefore, efficient screening of patients essential for general health. This study was performed to evaluate prospectively the diagnostic utility blood IgG antibody titer test against periodontal pathogens. Oral examination performed, titers pathogens were measured by ELISA in 1,387 individuals. The cut-off value determined receiver operating characteristic curve analysis,...
Glucocorticoids modulate the development and growth of many organs through interactions with a specific intracellular receptor (glucocorticoid receptor) that regulates gene transcription cognate element, glucocorticoid response element (GRE), in promoter target genes. In bone formation glucocorticoids stimulate osteoblast differentiation matrix. Recent studies have demonstrated induction sialoprotein (BSP) is associated de novo formation. To determine molecular pathways regulation BSP...
Proliferating cell nuclear antigen (PCNA), also called cyclin, was purified from PBS extract of rabbit thymus by using a combination ammonium sulfate fractionation, DEAE-Sephacel, HPLC ion exchange, and gel filtration column chromatography. PCNA more than 600 times analyzed sodium dodecyl polyacrylamide electrophoresis (SDS-PAGE) immunoblotting. SDS-PAGE showed that 36 kD protein selectively isolated in this purification process, identified as Other previously antigens, Sm, nRNP, SS-A/Ro,...
Background/Aims: The most prevalent infectious disease, chronic periodontitis which leads to alveolar bone destruction and subsequent tooth loss, develops due proinflammatory cytokine production induced by periodontopathic bacteria.Chronic obstructive pulmonary disease (COPD), a non-infectious is the third leading cause of death globally.This condition exacerbates frequently, attributable infection respiratory microorganisms such as Streptococcus pneumoniae.Although positive association has...
The junctional epithelium (JE) covers the cervical areas of developing or existing teeth. It can re-establish itself even after being removed during periodontal therapies, followed by wound healing. However, mechanisms that maintain this universally conserved structure are still unclear. molecular JE homeostasis were investigated altering levels JE-specific genes in a telomerase immortalized human gingival epithelial cell line (TIGKs) exposing TIGKs to conditioned medium (C-CM) from...
Transforming growth factor-beta (TGF-beta) increases steady-state mRNA levels of several extracellular matrix proteins in mineralized connective tissues. Bone sialoprotein (BSP) is a major constituent the bone matrix, thought to initiate and regulate formation mineral crystals. To determine molecular pathways TGF-beta 1 regulation proteins, we have analyzed effects on expression BSP rat osteosarcoma cell line (ROS 17/2.8). at ng/ml, increased ROS 17/2.8 cells approximately 8-fold:...
T o elucidate the characteristics of human periodontal ligament cells, we compared these cells with gingival fibroblasts isolated from tissues female subjects. Human (HPDL) had a sharper spindle shape and exhibited higher growth rate than (HGF). HPDL high level alkaline phosphatase (ALPase) activity, whereas HGF low such activity. Northern blot analysis demonstrated that produced ALPase mRNA. Decorin biglycan mRNA were detected in both HGF, osteocalcin bone sialoprotein was not either cells....
Bone sialoprotein (BSP), an early marker of osteoblast differentiation, has been implicated in the nucleation hydroxyapatite during de novo bone formation. Prostaglandin E2 (PGE2) anabolic effects on proliferation and differentiation osteoblasts via diverse signal transduction systems. Because PGE2 increases proportion functional fetal rat calvarial cell cultures, we investigated regulation BSP, as osteoblastic marker, by PGE2. Treatment osteosarcoma UMR 106 cells with 3 μm, 300 nm, 30 nm...
Bone sialoprotein (BSP), an early marker of osteoblast differentiation, has been implicated in the nucleation hydroxyapatite during bone formation de novo. Our studies, using osteoblastic cell line ROS 17/2.8, have revealed that rat BSP gene expression is suppressed by 1,25-dihydroxyvitamin D3[1,25(OH)2D3], which a powerful regulator and resorption. To determine molecular basis transcriptional suppression transcription 1,25(OH)2D3, we conducted transient transfection analyses with chimaeric...
A number of studies have recently suggested Epstein-Barr virus (EBV) involvement in the pathogenesis periodontitis. In this study, we investigated association between major periodontopathic bacteria Porphyromonas gingivalis (P. gingivalis) and EBV Japanese chronic periodontitis (CP) patients.A group 25 patients with CP participated study along 13 individuals without Subgingival samples were obtained paper points. Quantitative real-time polymerase chain reaction (PCR) was used to detect DNA...
Inflammatory cytokines may have important roles in periodontitis. We assessed the effects of initial periodontal therapy on clinical parameters and interleukin-1β (IL-1β) level gingival crevicular fluid (GCF) from chronic periodontitis (CP) patients. After screening, baseline such as probing pocket depth (PPD) bleeding (BOP) were measured. GCF samples collected 13 shallow (≤3 mm) deep (≥5 PPD sites CP patients, volume IL-1β concentration determined at (before scaling root planning) 2 4...
Peri-implantitis (PI) is an inflammatory reaction associated with functional deterioration of supporting bones around the dental implant. Recent studies suggested Epstein-Barr virus (EBV) involved in pathogenesis periodontitis. We investigated association between EBV and Porphyromonas gingivalis Japanese PI patients.Fifteen periodontally healthy individuals, 15 implant patients were recruited. Forty five subgingival plaque samples collected from deepest probing pocket depth (PPD) site each...
We investigated the prevalences and risk factors for peri-implant diseases in Japanese adult dental patients attending a follow-up visit at hospitals or clinics as part of their maintenance program. This cross-sectional multicenter study enrolled with implants who attended regular check-ups periodontal program during period from October 2012 through September 2013. Patients least 3 years loading time were included study. The condition tissue was examined classified into following categories:...