A5033901129- Oral microbiology and periodontitis research
- Bone and Dental Protein Studies
- Periodontal Regeneration and Treatments
- Nerve injury and regeneration
- Mesenchymal stem cell research
- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- dental development and anomalies
- Endodontics and Root Canal Treatments
- Bone Metabolism and Diseases
- Oral and gingival health research
- Oral Health Pathology and Treatment
- Barrier Structure and Function Studies
- Oral and Maxillofacial Pathology
- Connexins and lens biology
- Bone health and treatments
- Dental Radiography and Imaging
- NF-κB Signaling Pathways
- Signaling Pathways in Disease
- HIV/AIDS oral health manifestations
- Cell Adhesion Molecules Research
- Neuropeptides and Animal Physiology
- Bone Tissue Engineering Materials
- Probiotics and Fermented Foods
- Dental Implant Techniques and Outcomes
Hiroshima University
2016-2025
British Society of Periodontology
1997-2023
Hiroshima University Hospital
2020-2022
Higashihiroshima Medical Center
2022
Life Science Institute
2014-2015
In-Q-Tel
2012
Frontier Science Foundation
2011
American Association of Endodontists
2010
Frontier Medical College
2006-2007
University of Louisville
2003-2006
Background: The use of suitable cells transplanted into periodontal osseous defects appears to be a powerful strategy promote tissue regeneration. Mesenchymal stem (MSCs) isolated from bone marrow have the potential for multilineage differentiation. purpose this study was examine whether auto‐transplantation MSCs would useful Methods: Bone were beagle dogs and expanded in vitro. mixed with atelocollagen (2% type I collagen) at final concentrations 2 × 10 6 , 5 1 7 or cells/ml,...
The purpose of this study is to differentiate roles several growth factors and cytokines in proliferation differentiation pulp cells during development repair. In human cell cultures, laminin type I collagen levels per remained almost constant the whole culture period (22 days). On other hand, secreted protein, acidic rich cysteine (SPARC/osteonectin) alkaline phosphatase (ALPase) markedly increased after cultures reached confluence. Laminin collagen, as well fibronectin, stimulated...
Actinobacillus actinomycetemcomitans (Aa) is one of the pathogenic bacteria involved in periodontal diseases. We have previously identified six major outer membrane proteins (Omps) Aa Y4. Among them an Omp with high molecular mass, designated Omp100, which has homology to a variety virulence factors. Electron microscopic observation indicated that Omp100 randomly localized on cell surface Aa. Y4 been shown adhere and invade KB or normal human gingival keratinocytes. Anti-Omp100 antibody...
Abstract Osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) inhibits osteoclast differentiation, activity, and survival; therefore OPG/OCIF may regulate the resorption of dental hard tissues, such as alveolar bone, cementum, dentin. To investigate this issue, reverse transcriptase-polymerase chain reaction using specific primers for was performed with total RNAs isolated from human gingival keratinocytes (HGKs), fibroblasts (HGFs), periodontal ligament cells (HPDLs), pulp...
To address whether brain-derived neurotrophic factor (BDNF) could be involved in periodontal tissue regeneration, we examined the effects of BDNF on proliferation and expression bone (cementum)- related proteins (osteopontin, morphogenetic protein [BMP]-2, type I collagen, alkaline phosphatase [ALPase], osteocalcin) cultures human ligament (HPL) cells, which are thought to prerequisite for angiogenesis endothelial cells. Furthermore, effect regeneration tissues experimentally induced defects...
Brain-derived neurotrophic factor (BDNF), for which bovine collagen-derived atelocollagen is used as a scaffold, enhances periodontal tissue regeneration. However, scaffold that does not contain unknown ingredients preferable. Since the synthesized high-molecular-weight (HMW)-hyaluronic acid (HA) safe and inexpensive, we evaluated efficacy of HMW-HA BDNF scaffold. CD44, major receptor HA, was expressed in cultures human ligament cells, promoted adhesion proliferation although it did...
Brain-derived neurotrophic factor (BDNF) promotes the regeneration of periodontal tissue. Since angiogenesis is important for tissue regeneration, investigating effect BDNF on endothelial cell function may help to reveal its mechanism, whereby, regeneration. In this study, we examined influence migration in human microvascular cells (HMVECs), focusing effects extracellular signal-regulated kinase (ERK), integrin α(V)β(3), and focal adhesion (FAK). The was assessed with a modified Boyden...
The major problem in cell therapy is the possibility of viral or bacterial infection and immune reactions. Therefore, it expected culture cells which are intended to be re-implanted with autologous serum rather than conventional bovine serum. Cell human mesenchymal stem (hMSC), differentiating various cells, thought curative. To hMSC (HAS) re-implant them for therapy, we developed a completely closed bag system separating serum, comparing proliferation multipotency cultured HAS those foetal...
Brain-derived neurotrophic factor (BDNF), recognized as essential in the developing nervous system, is involved differentiation and proliferation non-neuronal cells, such endothelial osteoblasts, periodontal ligament cells. We have focused on application of BDNF to regeneration tissue indicated that promotes experimentally created defects. Cementoblasts form cementum, mineralized tissue, which key establishing a functional periodontium. The requires elucidation mechanism by regulates...
It is well-known that Enterococcus species produce bacteriocins have antibacterial activity. However, a comprehensive analysis of the bacteriocin distribution among strains has not been conducted. In this study, we identified genes from 80 faecalis and 38 faecium investigated their E. strains, cytolysin gene (61.3%), enterolysin A (27.5%) BacL1 (45.0%) were identified. enterocin (97.4%), B (2.6%), NKR-5-3B (21.0%), T8 (36.8%) BacAS9 (23.7%) The activity all was tested against faecium....
Staphylococcus aureus is a common bacterium that sometimes causes various pyogenic diseases. Methicillin resistant S. (MRSA) infections are particularly difficult to treat. Recently, MRSA has been spreading in the community, so it important determine prevalence of community and conduct epidemiological studies from genetic statistical perspectives. In this study, aureus/MRSA was isolated oral nasal cavities 504 dental patients (65 inpatients 439 outpatients). Sixty-two strains 9 were cavity,...
The purpose of this study is to evaluate whether β-tricalcium phosphate (β-TCP) could be a promising modality help augment alveolar bone in periodontal tissue regeneration by marrow mesenchymal stem cells (BMMSCs).Expanded BMMSCs and atelocollagen (Col) were mixed together (MSC/Col). A combination β-TCP with MSC/Col was also prepared (MSC/Col/TCP). MSC/Col/TCP or transplanted into experimental Class III furcation defects that had been exposed inflammation beagle dogs. Periodontal evaluated...
Elevated osteoclast (OC)-mediated bone resorption, a common pathological feature between periodontitis and rheumatoid arthritis (RA), implicates possible mutually shared pathogenesis. The autoantibody to citrullinated vimentin (CV), representative biomarker of RA, is reported promote osteoclastogenesis (OC-genesis). However, its effect on OC-genesis in the context remains be elucidated. In an vitro experiment, addition exogenous CV upregulated development Tartrate-resistant acid phosphatase...
Abstract To prevent nosocomial infection, it is important to screen for potential vancomycin‐resistant Enterococcus (VRE) among patients. In this study, we analyzed enterococcal isolates from inpatients in one hospital without any apparent outbreak of VRE. Enterococcal were collected at Hiroshima University Hospital April 1 June 30, 2021 using selective medium Enterococci. Multilocus sequence typing, antimicrobial susceptibility testing, and whole‐genome sequencing performed. A total 164...