A5042626772- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Bacterial Identification and Susceptibility Testing
- Bacteriophages and microbial interactions
- Biochemical and Structural Characterization
- Oral microbiology and periodontitis research
- Antimicrobial Peptides and Activities
- Antibiotic Use and Resistance
- Probiotics and Fermented Foods
- Dermatology and Skin Diseases
- Antibiotics Pharmacokinetics and Efficacy
- Clostridium difficile and Clostridium perfringens research
- Toxin Mechanisms and Immunotoxins
- Pharmaceutical and Antibiotic Environmental Impacts
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Peptidase Inhibition and Analysis
- Immune Response and Inflammation
- Genomics and Phylogenetic Studies
- Autoimmune Bullous Skin Diseases
- Enterobacteriaceae and Cronobacter Research
- Plant Pathogenic Bacteria Studies
- Vibrio bacteria research studies
Hiroshima University
2016-2025
National Institute of Infectious Diseases
2018-2025
Fujita Health University
2024
Kobe University
2024
International University of Health and Welfare
2024
Kumamoto Medical Center
2024
Hiroshima University Hospital
2024
Kyushu University
2009
Keio University
2004
Danish Ministry of Food, Agriculture and Fisheries
2004
Neutrophil extracellular traps (NETs) are webs of DNA covered with antimicrobial molecules that constitute a newly described killing mechanism in innate immune defense. Previous publications reported NETs take up to 3-4 h form via an oxidant-dependent event requires lytic death neutrophils. In this study, we describe neutrophils responding uniquely Staphylococcus aureus novel process NET formation did not require neutrophil lysis or even breach the plasma membrane. The multilobular nucleus...
BackgroundRecent studies have suggested that epidermal barrier dysfunction contributes to the development of atopic dermatitis (AD) and other allergic diseases.ObjectiveWe performed a prospective, randomized controlled trial investigate whether protecting skin with moisturizer during neonatal period prevents AD sensitization.MethodsAn emulsion-type was applied daily first 32 weeks life 59 118 neonates at high risk for (based on having parent or sibling AD) who were enrolled in this study....
Abstract The closure of skin wounds is essential for resistance against microbial pathogens, and keratinocyte migration an important step in wound healing. Cathelicidin hCAP18/LL-37 innate antimicrobial peptide that expressed the acts to eliminate pathogens. Because up-regulated at sites, we hypothesized LL-37 induces migration. In this study, found 1 μg/ml induced maximum level Boyden chamber assay. addition, phosphorylated epidermal growth factor receptor (EGFR) after 10 min, which...
The Tn551 insertion site of the autolysis-deficient Staphylococcus aureus mutant RUSAL2 was cloned and used to identify autolysis gene atl in parent strain, RN450. open reading frame for 3768 bp length, encoding a deduced protein 1256 amino acids molecular size 137,381 Da. product is bifunctional that has an amidase domain endo-beta-N-acetylglucosaminidase which must undergo proteolytic processing generate two extracellular lytic enzymes found culture broth S. aureus.
The purpose of this study was to identify guanine nucleotide-binding proteins (G proteins) involved in the agonist- and guanosine 5'-[gamma-thio]triphosphate (GTP[gamma-S])-induced increase Ca2+ sensitivity 20-kDa myosin light chain (MLC20) phosphorylation contraction smooth muscle. A constitutively active, recombinant val14p21rhoA.GTP expressed baculovirus/Sf9 system, but not protein without posttranslational modification Escherichia coli, induced at constant (pCa 6.4) a slow associated...
ABSTRACT The antimicrobial peptides human β-defensin-1 (hBD1), hBD2, hBD3, and CAP18 expressed by keratinocytes have been implicated in mediation of the innate defense against bacterial infection. To gain insight into Staphylococcus aureus infection, susceptibility S. , including methicillin-resistant (MRSA), to these was examined. Based on quantitative PCR, expression hBD2 mRNA significantly induced contact with hBD3 slightly induced, while hBD1 constitutively irrespective presence . Ten...
ABSTRACT We identified a novel pathogenicity island in Staphylococcus aureus which contains open reading frames (ORFs) similar to the exfoliative toxin (ET) gene, glutamyl endopeptidase and edin-B gene tandem phage resistance flanked by hsdM , hsdS (restriction modification system), IS 256 . The protein encoded ET-like showed 40, 59, 68% amino acid sequence identities with A (ETA), B (ETB), hyicus ETB (ShETB), respectively. When injected into neonatal mice, recombinant derived from induced...
We report the successful purification of a cloned lysin encoded by novel Staphylococcus aureus bacteriophage ϕMR11. The lysin, designated MV-L, rapidly and completely lysed cells number S. strains tested, including methicillin-resistant (MRSA) vancomycin-resistant subset vancomycin-intermediate (VISA) in growing conditions. MV-L–mediated killing is specific to not other species, except for simulans. MV-L exerted its staphylocidal effect synergistically with glycopeptide antibiotics against...
ABSTRACT Actinobacillus actinomycetemcomitans has been shown to produce a soluble cytotoxic factor(s) distinct from leukotoxin. We have identified in A. Y4 cluster of genes encoding cytolethal distending toxin (CDT). This new member the CDT family is similar produced by Haemophilus ducreyi . The was Escherichia coli and able induce cell distension, growth arrest G 2 /M phase, nucleus swelling, chromatin fragmentation HeLa cells. three proteins, CDTA, -B -C, encoded cdt locus were all...
atl is a newly discovered autolysin gene in Staphylococcus aureus. The product, ATL, unique, bifunctional protein that has an amidase domain and glucosaminidase domain. It undergoes proteolytic processing to generate two extracellular peptidoglycan hydrolases, 59-kDa endo-beta-N-acetylglucosaminidase 62-kDa N-acetylmuramyl-L-alanine amidase. been suggested these enzymes are involved the separation of daughter cells after cell division. We recently demonstrated products associated...
Cytotoxic necrotizing factor type 2 (CNF2) produced by Escherichia coli strains isolated from intestinal and extraintestinal infections is a dermonecrotic toxin of 110 kDa. We cloned the CNF2 gene large plasmid carried an strain lamb with septicemia. Hydropathy analysis deduced amino acid sequence revealed largely hydrophilic protein two potential hydrophobic transmembrane domains. The N-terminal half showed striking homology (27% identity 80% conserved residues) to portion Pasteurella...
Summary Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. (EHEC) are closely related pathogens. Both use a type III secretion system (TTSS) encoded by the ‘locus of enterocyte effacement’ (LEE) to subvert attach epithelial cells through injection repertoire effector molecules. Here, we report identification new TTSS translocated molecule called Cif, which blocks cell cycle G 2 /M transition induces formation stress fibres recruitment focal adhesions. Cif is not LEE but...
Staphylococcus aureus produces many virulence factors, including toxins, immune-modulatory and exoenzymes. Previous studies involving the analysis of expression were mainly performed by in vitro experiments using bacterial medium. However, when S. infects a host, growth conditions are quite different from those medium, which may be related to factors host. In this study, we investigated grown calf serum. The hemolysins, enterotoxins, proteases, iron acquisition was significantly increased...
Abstract Aim Oral microflora during pregnancy is critical to oral health care in the mother and her child. We examined changes microbiota between nonpregnancy periods. Methods The study was performed using 132 healthy pregnant women enrolled from Hiroshima City Asa Citizens Hospital 51 nonpregnant as control. During pregnancy, subjects were assessed for seven microbial species by cultured method polymerase chain reaction at early (7–16 weeks gestation), middle (17–28 weeks), late (29–39...
Abstract In bacterial genome and metagenome sequencing, Illumina sequencers are most frequently used due to their high throughput capacity, multiple library preparation kits have been developed for platforms. Here, we systematically analysed compared the sequencing bias generated by currently available sequencing. Our analyses revealed that a strong is introduced in low-GC regions Nextera XT kit. The level of dependent on GC content; stronger as content decreases. Other did not introduce...
Staphylococcal enterotoxins (SEs) produced by Staphylococcus aureus have superantigenic and emetic activities, which cause toxic shock syndrome staphylococcal food poisoning, respectively. Our previous study demonstrated that the sequence of SET has a low level similarity to sequences other SEs exhibits atypical bioactivities. Hence, we further explored whether there is an additional SET-related gene in S. strains. One SET-like was found genome isolates originated from case human nasal swab,...
There has been scarce evidence about deaths due to blood stream infection (BSI) in Japan so far. The main objective of this study is understand the epidemiological trend caused by BSIs Staphylococcus aureus and Escherichia coli including Methicillin-resistant S. (MRSA) fluoroquinolone-resistant E. (FQREC) at national level. We annually estimated number BSI between 2011 2017 across using comprehensive data bacterial culturing drug susceptibilities collected Nosocomial Infection Surveillance...
Prophages are often involved in host survival strategies and contribute toward increasing the genetic diversity of genome. also drive horizontal propagation various genes as vehicles. However, there few retrospective studies contributing to antimicrobial resistance (AMR) virulence factor (VF) by prophage. We extracted complete genome sequences seven pathogens, including ESKAPE bacteria Escherichia coli from a public database, examined distribution both AMR VF prophage-like regions. found...
The impact of the coronavirus disease (COVID-19) pandemic on antimicrobial resistance (AMR) is a major concern.To compare number patients and isolation rate antimicrobial-resistant bacteria before after beginning COVID-19 using comprehensive national surveillance data.We utilized data, collected in Japan Nosocomial Infections Surveillance programme, which included total 16.7 million samples 5.9 tested from >1300 hospitals. We compared five between 2019 2020, including...
Japan is a country with an approximate 10% prevalence rate of carbapenem-resistant
In this study, we evaluated the permeation of piperacillin (PIPC), imipenem (IPM), amikacin (AKM), gentamicin (GM), ofloxacin (OFLX), levofloxacin (LVFX), ciprofloxacin (CPFX) and sparfloxacin (SPFX) through Pseudomonas aeruginosa biofilm with a simple new method. Bacteria used were leucine-requiring mucoid mutant. grown on membrane cell culture insert in chemically defined medium incubated at 37 degrees C for 5 days. At days 0, 1, 3 5, penetration rates biofilms measured. PIPC IPM...