A5007882770- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Vibrio bacteria research studies
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Aquaculture disease management and microbiota
- Probiotics and Fermented Foods
- Insect Utilization and Effects
- Plant Virus Research Studies
- Polyomavirus and related diseases
- Glycosylation and Glycoproteins Research
- Helicobacter pylori-related gastroenterology studies
- Enzyme Production and Characterization
- Ocular Infections and Treatments
- Insects and Parasite Interactions
- Insect behavior and control techniques
- Enzyme Structure and Function
- Antimicrobial Resistance in Staphylococcus
- Full-Duplex Wireless Communications
- Agriculture and Biological Studies
- Microbial Metabolites in Food Biotechnology
- Cancer Research and Treatments
Kochi Gakuen College
2008-2024
Kōchi University
2013-2024
Kochi Medical School Hospital
1999-2019
Biology of Infection
2010-2012
Czech Academy of Sciences, Institute of Microbiology
2003-2006
Harvard University
1999
University of Kochi
1999
Hiroshima University
1982-1988
The protective effects of bacteriophages were assessed against experimental Staphylococcus aureus infection in mice. Of the S. phages isolated study, ϕMR11 was representatively used for all testing, because its host range most broad and it carries no genes known toxins or antibiotic resistance. Intraperitoneal injections (8×108 cells) including methicillin-resistant bacteria, caused bacteremia eventual death In contrast, subsequent intraperitoneal administration purified (MOI ⩾0.1)...
We report the successful purification of a cloned lysin encoded by novel Staphylococcus aureus bacteriophage ϕMR11. The lysin, designated MV-L, rapidly and completely lysed cells number S. strains tested, including methicillin-resistant (MRSA) vancomycin-resistant subset vancomycin-intermediate (VISA) in growing conditions. MV-L–mediated killing is specific to not other species, except for simulans. MV-L exerted its staphylocidal effect synergistically with glycopeptide antibiotics against...
We evaluated the efficacy of bacteriophage (phage) therapy by using a murine model gut-derived sepsis caused Pseudomonas aeruginosa that closely resembles clinical pathophysiology septicemia in humans. Oral administration newly isolated lytic phage strain (KPP10) significantly protected mice against mortality (survival rates, 66.7% for phage-treated group versus 0% saline-treated control group; P<0.01). Mice treated with also had lower numbers viable P. cells their blood, liver, and spleen....
The therapeutic effects of bacteriophage (phage) KPP12 in Pseudomonas aeruginosa keratitis were investigated mice. Morphological analysis showed that phage is a member the family Myoviridae, morphotype A1, and DNA sequence revealed similar to PB1-like viruses. Analysis genome did not identify any genes related drug resistance, pathogenicity or lysogenicity, so may be good candidate for therapeutic. broad host range P. strains isolated from clinical ophthalmic infections. Inoculation...
The emergence of multi-drug resistant Pseudomonas aeruginosa necessitates the search for treatment options other than antibiotic use. use bacteriophages is currently being considered as an alternative to antibiotics bacterial infections. A number were introduced treat pneumonia in past reports. However, there are still lack knowledge regarding dosages, application time, mechanism and safety phage therapy against P. pneumonia. We used bacteriophage KPP10 strain D4-induced mouse models...
Along with the increasing threat of nosocomial infections by vancomycin-resistant Enterococcus faecalis, bacteriophage (phage) therapy has been expected as an alternative against infectious disease. Although genome information and proof applicability are prerequisites for a modern therapeutic phage, E. faecalis phage not analyzed in terms these aspects. Previously, we reported novel virulent phiEF24C, its biology indicated potential infection. In this study, phiEF24C was vivo also briefly...
Helicobacter pylori inhabits the stomach mucosa and is a causative agent of ulcer cancer. In general, bacteriophages (phages) are strongly associated with bacterial evolution, including development pathogenicity. Several tailed phages have so far been reported in H. pylori. We isolated an phage, KHP30, its genomic sequence. this study, we examined biological characteristics phage KHP30. Phage KHP30 was found to be spherical lipid-containing diameter ca. 69 nm. Interestingly, it stable from...
Some bacterial strains of the multidrug-resistant Gram-positive bacteria Enterococcus faecalis can significantly reduce efficacy conventional antimicrobial chemotherapy. Thus, introduction bacteriophage (phage) therapy is expected, where a phage used as bioagent to destroy bacteria. E. ΦEF24C known be good candidate for therapeutic against faecalis. However, this still produces nonuniform effects with different same species and might prove detrimental its effects. One solution problem...
ABSTRACT Helicobacter pylori causes peptic ulcers and gastric cancer, which lead to significantly higher morbidity in Japan than elsewhere the world. As bacteriophage (phage) host bacteria coevolve, study of H. phages is important extend understanding evolution pathogenesis . Here we report two complete genome sequences KHP30 KHP40, were released spontaneously from most pathogenic East Asian-type isolates Japanese patients.
A broad-host-range vibriophage, KVP40, was isolated from sea water by using Vibrio parahaemolyticus 1010 (EB101) as the indicator host. The host range of KVP40 extended over at least 8 and 1 Photobacterium species. a large tailed phage containing double-stranded DNA belonged to Ackermann's morphotype A2. cleaved 11 different type II restriction endonucleases including EcoRI HindIII, but not 17 other enzymes BamHI, KpnI SalI.
A tailed bacteriophage, phi MR11 (siphovirus), was selected as a candidate therapeutic phage against Staphylococcus aureus infections. Gene 61, one of the 67 ORFs identified, is located in morphogenic module. The gene product (gp61) has lytic domains homologous to CHAP (corresponding an amidase function) at its N-terminus and lysozyme subfamily 2 (LYZ2) C-terminus. Each domain gp61 purified recombinant protein. Both [amino acids (aa) 1-150] (aa 401-624) but not linker 151-400) caused...
Silkworm larva has recently been recognized as an alternative model animal for higher mammals to evaluate the effects of antibiotics. In this study, we examined efficacy bacteriophage (phage) therapy, which harnesses phages antibacterial agents, against Staphylococcus aureus infections, using silkworm larval infection model. Two newly isolated staphylococcal phages, S25-3 and S13', were used therapeutic phage candidates. They assigned two different lytic genera, Twort-like AHJD-like viruses,...
ABSTRACT In bacteriophage (phage) therapy against Gram-positive bacteria, such as Staphylococcus aureus , Listeria monocytogenes and Enterococcus faecalis members of a genus SPO1-like viruses are typically employed because their extreme virulence broad host spectrum. Phage φEF24C, which is virus infecting E. has previously been characterized therapeutic phage candidate. addition to the itself, endolysin also recognized an effective antimicrobial agent. this study, putative gene ( orf9 )...
Impetigo is a contagious skin infection predominantly caused by Staphylococcus aureus. Decontamination of S. aureus from the becoming more difficult because emergence antibiotic-resistant strains. Bacteriophage endolysins are less likely to invoke resistance and can eliminate target bacteria without disturbance normal microflora. In this study, we investigated therapeutic potential recombinant endolysin derived kayvirus S25-3 against staphylococcal impetigo in an experimental setting. First,...
KVP40 is a broad-host-range vibriophage forming plaques on strains of at least eight Vibrio and one Photobacterium species. A spontaneous KVP40-resistant mutant, R4000, derived from parahaemolyticus 1010 lacked 26-kDa outer membrane protein designated OmpK. was inactivated by OmpK prepared 1010, but not R4000. These results strongly suggest that the receptor for KVP40. Immunoblotting analyses using an anti-OmpK rabbit serum revealed or its homologs molecular masses 25-29 kDa were distributed...