A5080526180- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Immune Response and Inflammation
- Bacterial Identification and Susceptibility Testing
- Cardiac Imaging and Diagnostics
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Clostridium difficile and Clostridium perfringens research
- Urinary Tract Infections Management
- Antibiotic Use and Resistance
- Bacterial Infections and Vaccines
- Streptococcal Infections and Treatments
- Cardiovascular Function and Risk Factors
- Nitric Oxide and Endothelin Effects
- Nephrotoxicity and Medicinal Plants
- Legionella and Acanthamoeba research
- Mycobacterium research and diagnosis
- Infective Endocarditis Diagnosis and Management
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Salmonella and Campylobacter epidemiology
- Microbial infections and disease research
- Cardiovascular Health and Disease Prevention
International University of Health and Welfare
2018-2025
Japan Society of Chemotherapy
2013-2024
Tokyo Medical University
2013-2022
Osaka Kyoiku University
2011-2020
Osaka Kaisei Hospital
2020
Kitasato University
2020
Tokyo Medical University Hachioji Medical Center
2020
Saiseikai Ibaraki Hospital
2020
Nagoya University
2018
Tokyo Medical University Hospital
2011-2017
ABSTRACT Quorum-sensing systems are critical regulators of the expression virulence factors various organisms, including Pseudomonas aeruginosa . Las and Rhl two major quorum-sensing components, they regulated by their corresponding autoinducers, N -3-oxododecanoyl homoserine lactone (3-oxo-C 12 -HSL) -butyryl- l -homoserine (C 4 -HSL). Recent progress has demonstrated potential molecules, especially 3-oxo-C -HSL, for modulation host immune system. Here we show specific ability -HSL to...
This study was designed to define the lipopolysaccharide (LPS) sensitivity of aged mice in terms lethality and cytokine production determine down-regulating responses corticosterone interleukin 10 (IL-10). The 50% lethal doses LPS young (6- 7-week-old) (98- 102-week-old) were 601 93 microg per mouse (25.6 1.6 mg kg body weight), respectively. Aged approximately 6.5-fold more sensitive toxicity micrograms (16-fold milligrams kilogram) than mice. Levels sera tumor necrosis factor-alpha...
We evaluated the efficacy of bacteriophage (phage) therapy by using a murine model gut-derived sepsis caused Pseudomonas aeruginosa that closely resembles clinical pathophysiology septicemia in humans. Oral administration newly isolated lytic phage strain (KPP10) significantly protected mice against mortality (survival rates, 66.7% for phage-treated group versus 0% saline-treated control group; P<0.01). Mice treated with also had lower numbers viable P. cells their blood, liver, and spleen....
The accumulation of certain species bacteria in the intestine is involved both tissue homeostasis and immune-mediated pathologies. host mechanisms controlling intestinal colonization with commensal are poorly understood. We observed that under specific pathogen-free or germ-free conditions, intragastric administration Pseudomonas aeruginosa, E. coli, Staphylococcus aureus, Lactobacillus gasseri resulted increased small bacterial translocation mice lacking Cd1d, an MHC class I-like molecule,...
The purpose of this study was to identify the clinical characteristics and outcomes peripheral vascular catheter-related bloodstream infections (PVC-BSIs) determine risk severe complications or death. We performed a retrospective observational from June 2010 April 2015 at two regional university-affiliated hospitals in Tokyo. studied manifestations, underlying diseases, laboratory results, treatment methods, recurrence rates, 62 hospitalized patients diagnosed with PVC-BSIs by positive blood...
Veterinary medications are necessary for both contemporary animal husbandry and food production, but their residues can linger in foods obtained from animals pose a dangerous human risk. In this review, we aim to highlight the sources, occurrence, exposure pathways, health effects of drug food-animal products. Following usage veterinary medications, pharmacologically active compounds known as be found food, environment, or animals. They cause major concerns people, including antibiotic...
The production and role of tumor necrosis factor alpha (TNF-alpha) in pneumococcal pneumonia were investigated a mouse model. When approximately 10(6) CFU Streptococcus pneumoniae TUM19 used to inoculate CBA/J mice intranasally, TNF-alpha levels the lungs serum began increase from 1 3 days after infection, respectively, concomitantly with bacterial counts lungs. Anti-TNF-alpha antibody accelerated proliferation blood death mice. Although immunoglobulin G against infecting bacteria not...
When oral tolerance was induced in either specific pathogen-free (SPF) or germ-free (GF) mice, ovalbumin (OVA) feeding before immunization successfully SPF mice. On the other hand, OVA-specific immunoglobulin G1 (IgG1) and IgE titres OVA-fed GF mice were comparable to those phosphate-buffered saline-fed thus demonstrating that could not be The frequencies of CD25(+) CD4(+)/CD4(+) cells mesenteric lymph node (MLN) absolute number CD4(+) Peyer's patches MLN naive significantly lower than In an...
Oral intake of Lactobacillus pentosus strain b240 (b240) has been shown to enhance the secretion salivary secretory IgA in elderly adults. However, its clinical benefits remain be determined. We tested hypothesis that exerts a protective effect against common cold The design present study was randomised, double-blind, placebo-controlled trial (RCT) with parallel three-group comparison. For this purpose, 300 eligible adults were randomly allocated one three groups, namely placebo, low-dose or...
Emergency myelopoiesis is inflammation-induced hematopoiesis to replenish myeloid cells in the periphery, which critical control infection with pathogens. Previously, pro-inflammatory cytokines such as interferon (IFN)-α and IFN-γ were demonstrated play a role expansion of hematopoietic stem (HSCs) progenitors, leading production mature cells, although their inhibitory effects on also reported. Therefore, molecular mechanism emergency during remains incompletely understood. Here, we clarify...
ObjectivesMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a fast and reliable method for the identification of bacteria. A MALDI Sepsityper kit generally used to prepare samples obtained directly from culture bottles. However, relatively high cost this major obstacle introducing into routine clinical use. In study, accuracies three different preparation methods rapid direct bacteria positive blood bottles by MALDI-TOF MS analysis were...
Although community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged worldwide, no nationwide CA-MRSA surveillance been conducted in Japan to determine the changes its molecular characteristics over time. We aimed characterize epidemiology of Panton-Valentine leucocidin (PVL)-positive strains collected from across past decade. isolated 1,770 MRSA skin and pus samples outpatients 244 medical facilities 31 prefectures between 2010 2018 (2010, 2012, 2014, 2016, 2018)....
Several previous investigators have reported that long-term administration of certain macrolides is efficacious in patients with persistent pulmonary Pseudomonas aeruginosa infections, even though the clinically achievable concentrations these medications are far below their MICs. In present study, we examined how sub-MICs macrolide antibiotics affect viability and protein synthesis several strains P. aeruginosa. We report 48 h, but not 12 or 24 growth on agar containing a concentration...
The use of high doses the anti-cancer drug methotrexate (MTX) is associated with intestinal damage. As a result, mucosal immune cells become increasingly exposed to vast amount microbial stimuli. We aimed at determining whether these are still functional during MTX treatment. Furthermore, we assessed if activation system would play role in pathogenesis mucositis. A contributive mucositis for adaptive was established by showing that lymphocytes from MTX-treated mice secreted enhanced amounts...
ABSTRACT Helicobacter cinaedi has being recognized as an important human pathogen which causes bloodstream infections. Although the first case of bacteremia with this in Japan was reported 2003, true prevalence H. a infections country is not yet known. Therefore, aim our study to assess incidence Japan. We conducted prospective, multicenter analysis 13 hospitals during 6 months Tokyo, Among positive blood cultures from 1 October 2003 31 March 2004, isolates suspected species were studied for...
This study was designed to investigate, at the molecular level, antimicrobial resistance mechanisms of different genes, including, extended-spectrum β-lactamases, AmpC class 1 and 2 integrons, plasmid-mediated quinolone genes Gram-negative bacteria isolated from clinical settings in Egypt. A total 126 nonduplicate isolates were recovered samples taken hospitalized patients Egypt 2014. Antimicrobial susceptibility testing showed that, 93.6% (118/126) had a multidrug-resistant phenotype....
The emergence of multi-drug resistant Pseudomonas aeruginosa necessitates the search for treatment options other than antibiotic use. use bacteriophages is currently being considered as an alternative to antibiotics bacterial infections. A number were introduced treat pneumonia in past reports. However, there are still lack knowledge regarding dosages, application time, mechanism and safety phage therapy against P. pneumonia. We used bacteriophage KPP10 strain D4-induced mouse models...
To investigate the genetic basis of carbapenem resistance in clinical S. marcescens isolates collected from patients Japan between 1994 and 2016. 5135 were recovered different medical centers across Japan, identified central laboratories, tested for antimicrobial agents using broth microdilution method. All that showed intermediate or resistant phenotypes at least one antibiotic confirmed by susceptibility testing carbapenemase production modified inactivation method (mCIM). Furthermore,...