Summary mRNA interferases are sequence‐specific endoribonucleases encoded by toxin‐antitoxin (TA) systems in bacterial genomes. Previously, we demonstrated that Mycobacterium tuberculosis contains at least seven genes encoding MazF homologues (MazF‐mt1 to ‐mt7) and determined cleavage specificities for MazF‐mt1 MazF‐mt6. Here have developed a new general method the determination of recognition sequences longer than three bases with use phage MS2 RNA as substrate CspA, an chaperone, which...

Gram-positive bacteria possess a thick cell wall composed of mesh polymer peptidoglycans, which provides physical protection. Endolysins encoded by phages infecting can hydrolyse peptidoglycans in the bacterial wall, killing host immediately. The endolysin (Psm) episomal phage phiSM101 enterotoxigenic Clostridium perfringens type A strain SM101 exhibits potent lytic activity towards most strains perfringens. Psm has an N-terminal catalytic domain highly homologous to N-acetylmuramidases...

This study was designed to investigate, at the molecular level, antimicrobial resistance mechanisms of different genes, including, extended-spectrum β-lactamases, AmpC class 1 and 2 integrons, plasmid-mediated quinolone genes Gram-negative bacteria isolated from clinical settings in Egypt. A total 126 nonduplicate isolates were recovered samples taken hospitalized patients Egypt 2014. Antimicrobial susceptibility testing showed that, 93.6% (118/126) had a multidrug-resistant phenotype....

Summary Pkn8 is a membrane‐associated protein Ser/Thr kinase (PSTK) of Myxoccocus xanthus that was previously found to associate with novel cytoplasmic kinase, Pkn14. In the present study, MrpC, an essential transcription factor for fruA expression during fruiting body development, identified using genomic yeast two‐hybrid screen Pkn14 as bait. Our biochemical studies demonstrated purified and are active kinases able phosphorylate forms tetramer via its C‐terminal 41 residues. Moreover,...

Summary The developmental process of Myxococcus xanthus is achieved by the expression a specific set genes under influence signals. MrpC member CRP family transcription regulators, essential for fruA during development. Pkn8‐Pkn14 protein kinase cascade negatively regulates mrpC (H. Nariya and S. Inouye, 2005. Mol Microbiol 58: 367–379). Elevated levels in pkn8 pkn14 deletion strains (Δ Δ ) induce untimely FruA production vegetative growth resulting significantly faster fruiting body...

Many pathogenic clostridial species produce toxins and enzymes. To facilitate genome-wide identification of virulence factors biotechnological application their useful products, we have developed a markerless in-frame deletion method for Clostridium perfringens which allows efficient counterselection multiple-gene disruption. The system comprises galKT gene disruptant suicide galK plasmid into two fragments target are cloned. was shown to be accurate simple by using it disrupt the...

Abstract: This study describes the first full genomic sequence of an mcr-9 and blaVIM-4-carrying multidrug-resistant Enterobacter hormaechei clinical isolate from Egypt. The strain was isolated in April 2015 sputum a patient Cairo, blaVIM-4 genes were identified by PCR screening DNA sequencing; subjected to antimicrobial susceptibility testing, conjugation experiments, whole sequencing. carried IncHI2 plasmid, pAMS-38a (281,121 bp size); plasmid also conferring resistance against...

Phase changes in Bacteroides fragilis, a member of the human colonic microbiota, mediate variations vast array cell surface molecules, such as capsular polysaccharides and outer membrane proteins through DNA inversion. The results present study show that vesicle (OMV) formation this anaerobe is also controlled by inversions at two distantly localized promoters, IVp-I IVp-II are associated with extracellular polysaccharide biosynthesis expression proteins. These promoter mediated single...

Staphylococcal bi‐component toxins, leukocidin and γ‐hemolysin, consist of two protein components, i.e. F S for HγI HγII γ‐hemolysin. In this study we purified to homogeneity from the culture medium Staphylococcus aureus RIMD 310925 compared their properties with those same source. The N‐terminal 59‐ C‐terminal 2‐residue amino acid sequences, apparent molecular mass, isoelectri point were as F. an Ouchterlony double diffusion test a fused line without spur was formed between using either...

A xylose-inducible gene expression vector for Clostridium perfringens was developed. Plasmid pXCH contains a chromosomal region from difficile (xylR-P(xy)(lB)): xylR, encoding the xylose repressor, xylO, xyl operator sequence, and P(xylB), divergent promoter upstream of xylBA xylulo kinase isomerase. allows tightly regulated chloramphenicol acetyltransferase reporter α-toxin genes in response to inducer concentration. Thus, could constitute new valuable genetic tool study C. perfringens.

We describe here the complete genome sequence of an Enterobacter hormaechei ST279 coharbouring blaVIM-1 and mcr-9 recovered from uncooked beef patty in June 2017, Egypt. The tested isolate was resistant to carbapenem but susceptible colistin (minimum inhibitory concentration (MIC), 0.5 μg/mL). antimicrobial susceptibility profile conjugation experiments were performed. entire sequenced by Illumina MiniSeq Oxford Nanopore methods. genes are carried on same IncHI2/pMLST1 plasmid, pMS37a (Size...

We developed two multiplex polymerase chain reactions (PCRs) for the detection of extended-spectrum β-lactamases (ESBLs), plasmid-mediated AmpC β-lactamases, aac(6')-Ib gene, and integrase genes (intI1, intI2, intI3) in class 1, 2, 3 integrons Gram-negative bacteria. evaluated PCRs using 109 isolates from non-organic (ANO) organic (AO) vegetables fruits. Screening ANO substances identified five SHV, one TEM-1, CTX-M, 20 AmpC-CS, intI1 positives. DNA sequencing revealed CTX-M Pantoea spp. was...

Dental plaque formed on tooth surfaces is a complex ecosystem composed of diverse oral bacteria and salivary components. Accumulation dental risk factor for caries periodontal diseases. L-arginine has been reported to decrease the by elevating pH through activity arginine deiminase in bacteria. Here we evaluated potential remove established biofilms.Biofilms were using human saliva mixed with Brain Heart Infusion broth supplemented 1 % sucrose multi-well plates or plastic discs. After...

The aim of this study was to investigate the occurrence plasmid-encoded colistin resistance among Gram-negative bacteria isolated from meat and products in Egypt report draft genome sequence anmcr-1/IncI2-carrying multidrug-resistant (MDR) Escherichia coli B1:ST101 isolate. A total 128 colistin-resistant strains were various product samples different cities Egypt. Multiplex PCR screening for plasmid-mediated genes performed. Whole-genome sequencing performed using an Illumina NextSeq...

Myxococcus xanthus is a gram-negative bacterium that forms multicellular fruiting bodies upon starvation. Here, we demonstrate it contains at least 13 eukaryotic-like protein Ser/Thr kinases (Pkn1 to Pkn13) individually having unique features. All contain the kinase domain of approximately 280 residues near N-terminal end, which share highly conserved features in eukaryotic kinases. The followed by putative regulatory consisting 185 692 residues. These domains no significant sequence...

Summary Myxococcus xanthus is a Gram‐negative bacterium that exhibits communal lifestyle during vegetative growth and multicellular development, forming fruiting bodies filled with spores. It contains at least 13 eukaryotic‐like protein Ser/Thr kinases (PSTKs from Pkn1 to Pkn13). In the present report, we demonstrate Pkn4, gene located 18 bp downstream of for 6‐phosphofructokinase (PFK), PSTK M. PFK (Mx‐PFK), key regulatory enzyme in glycolysis. Both Pkn4 Mx‐PFK were expressed Escherichia...

The increasing spread of carbapenem resistance is a serious global public health concern that negatively affects human and animal health. In this study we characterized the carbapenemase production in gram-negative bacteria isolated from different meat products Egypt. Phenotypic genotypic susceptibility testing were investigated. Two Enterobacter cloacae complex strains, kofta beef burger, one Pseudomonas aeruginosa minced meat, found to harbor VIM-1 VIM-2, respectively. These isolates...

Clostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. Potential treatments include phage-derived endolysins, a promising family of alternative antimicrobial agents. We surveyed the genome C. st13 strain and identified endolysin gene, psa, in phage remnant region. Psa has N-terminal catalytic domain homologous amidase_2 domain, C-terminal unknown function. psa gene derivatives encoding...