A5030465724- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Heat shock proteins research
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Enzyme Production and Characterization
- Protein purification and stability
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Lipid Membrane Structure and Behavior
- DNA and Nucleic Acid Chemistry
- Escherichia coli research studies
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- DNA Repair Mechanisms
- Cellular transport and secretion
- thermodynamics and calorimetric analyses
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Chromatin Dynamics
- Biochemical and Molecular Research
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
Rutgers, The State University of New Jersey
2011-2024
Johnson University
2014-2024
Rutgers Sexual and Reproductive Health and Rights
2010-2021
Chung-Ang University
2012
Structural Genomics Consortium
2009-2010
Columbia University
2005
Keio University
1986-2005
McGill University
2000
University of California, Berkeley
1979-1998
Universidad de León
1998
The expression of the genes for major outer membrane proteins OmpF and OmpC are osmoregulated. ompC locus was found to be transcribed bidirectionally under conditions high osmolarity a 174-base transcript encoded upstream inhibit production substantially reduce amount ompF mRNA. This RNA [mRNA-interfering complementary (micRNA)] has long sequence that is 5' end region We propose micRNA inhibits translation mRNA by hybridizing with it. interaction may cause premature termination transcription...
CspA, the major cold-shock protein of Escherichia coli, is dramatically induced during response. The amino acid sequence CspA shows 43% identity to “cold-shock domain” eukaryotic Y-box family, which interacts with RNA and DNA regulate their functions. Here, we demonstrate that binds as a chaperone. First, cooperatively heat-denatured single-stranded if it larger than 74 bases, causing supershift in gel electrophoresis. A minimal concentration at 2.7 × 10−5M absolutely required for this...
When exponentially growing Escherichia coli cell cultures were transferred from 37 degrees C to 10 or 15 C, the production of a 7.4-kDa cytoplasmic protein (CS7.4) was prominently induced. The rate CS7.4 reached 13% total synthesis within 1-1.5 hr after shift and subsequently dropped lower basal level. Regulation expression very strict, such that undetectable at C. We have cloned gene encoding this completed nucleotide sequence analysis, which revealed encodes hydrophilic 70 amino acid residues.
Multicopy single-stranded DNA (msDNA), a branched DNA-RNA molecule, has been shown in Escherichia coli B and clinical strain Cl-1 to be synthesized by reverse transcriptase. We report that 13% of the strains ECOR collection, sample 72 E. isolates representing breadth genetic variation species, produce msDNA. Three four major subspecific groups include msDNA-producing strains. Screening 25 are genetically related uncovered 22 additional A phylogenetic tree based on allelic detected...
The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
The envelope of Escherichia coli consists two distinct membranes, the outer membrane and cytoplasmic membrane. space between membranes is called periplasmic space, each fraction contains its own specific proteins. In this review, it discussed how proteins are localized in their final locations envelope. Proteins as well transmembranous appear to be produced from precursors which have peptide extensions about 20 amino acid residues at terminal ends. General features for extension deduced...
Subtilisin E, an alkaline serine protease of Bacillus subtilis 168, is first produced as a precursor, pre-pro-subtilisin, which consists signal peptide for protein secretion (pre-sequence) and extension 77 amino acid residues (pro-sequence) between the mature subtilisin. When entire coding region pre-pro-subtilisin E was cloned into Escherichia coli expression vector, active subtilisin secreted periplasmic space. pre-sequence replaced with E. OmpA peptide, also produced. directly fused to...
The messenger RNA for the lipoprotein of E. coli outer membrane was found to code a putative precursor, prolipoprotein, which has 20 additional amino acid residues extending from terminus lipoprotein. Using prolipoprotein synthesized in an cell-free system directed by purified lipoprotein, complete sequence amino-terminal precursor region determined be as follows: (formula: see text). It also that accumulates toluene-treated cells same sequence. significance is discussed terms mechanism...
CspA, the major cold-shock protein of Escherichia coli , is an RNA chaperone, which thought to facilitate translation at low temperature by destabilizing mRNA structures. Here we demonstrate that as well homologous chaperones CspE and CspC, are transcription antiterminators. In vitro addition physiological concentrations recombinant CspE, or CspC decreased termination several intrinsic terminators also pausing. vivo overexpression cloned 37°C was sufficient induce metY - rpsO operon genes...
The major cold shock protein of Escherichia coli, CspA, produced upon a rapid downshift in growth temperature, is involved the transcriptional regulation at least two genes. shares high homology with nucleic acid-binding domain Y-box factors, family eukaryotic proteins and translational regulation. crystal structure CspA has been determined 2-A resolution refined to R = 0.187. composed five antiparallel beta-strands forming closed five-stranded beta-barrel. three-dimensional similar that...
A 70-kDa protein was specifically induced in Escherichia coli when the culture temperature shifted from 37 to 15 degrees C. The identified be product of deaD gene (reassigned csdA) encoding a DEAD-box protein. Furthermore, after shift C, CsdA exclusively localized ribosomal fraction and became major ribosomal-associated cells grown at csdA deletion significantly impaired cell growth synthesis number proteins, derepression heat-shock low temperature. Purified found unwind double-stranded RNA...