A5041340974- Cancer Genomics and Diagnostics
- Radiomics and Machine Learning in Medical Imaging
- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- interferon and immune responses
- Nanoparticle-Based Drug Delivery
- Chemical Reactions and Isotopes
- Immune cells in cancer
- Cell Image Analysis Techniques
- Computational Drug Discovery Methods
- Biosimilars and Bioanalytical Methods
- Cancer, Hypoxia, and Metabolism
- Advanced Biosensing Techniques and Applications
- Cytokine Signaling Pathways and Interactions
- Molecular Biology Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Ferroptosis and cancer prognosis
- Neurobiology and Insect Physiology Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Intelligent Tutoring Systems and Adaptive Learning
- Medical Imaging Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Tuberculosis Research and Epidemiology
Marine Biological Laboratory
2022-2024
University of Virginia
2023-2024
University of Washington
2015-2024
Nanostring Technologies (United States)
2023-2024
Bruker (United States)
2024
Cognition Therapeutics (United States)
2021-2024
Harvard University
2011-2019
Boston Children's Hospital
2011-2019
Boston VA Research Institute
2019
University of Technology Sydney
2017
Abstract Background High-throughput RNA-Sequencing (RNA-Seq) has become the preferred technique for studying gene expression differences between biological samples and discovering novel isoforms, though techniques to analyze resulting data are still immature. One pre-processing step that is widely but heterogeneously applied trimming, in which low quality bases, identified by probability they called incorrectly, removed. However, impact of trimming on subsequent alignment a genome could...
Abstract Introduction Amyloid beta (Aβ) oligomers are one of the most toxic structural forms Aβ protein and hypothesized to cause synaptotoxicity memory failure as they build up in Alzheimer's disease (AD) patients’ brain tissue. We previously demonstrated that antagonists sigma‐2 receptor complex effectively block oligomer toxicity. CT1812 is an orally bioavailable, penetrant small molecule antagonist appears safe well tolerated healthy elderly volunteers. tested CT1812's effect on...
RNA-Seq has supplanted microarrays as the preferred method of transcriptome-wide identification differentially expressed genes. However, analysis is still rapidly evolving, with a large number tools available for each three major processing steps: read alignment, expression modeling, and Although some studies have benchmarked these against gold standard gene sets, few evaluated their performance in concert one another. Additionally, there general lack testing such on real-world,...
At the cellular level, α-tubulin acetylation alters structure of microtubules to render them mechanically resistant compressive forces. How this biochemical property microtubule relates mechanosensation remains unknown, although prior studies have shown that influences touch perception. Here, we identify major Drosophila acetylase (dTAT) and show it plays key roles in several forms mechanosensation. dTAT is highly expressed larval peripheral nervous system (PNS), but largely dispensable for...
RNA-Sequencing analysis methods are rapidly evolving, and the tool choice for each step of one common workflow, differential expression analysis, which includes read alignment, modeling, differentially expressed gene identification, has a dramatic impact on performance characteristics. Although number workflows emerging as high performers that robust to diverse input types, relative characteristics these when either depth or sample is limited–a occurrence in real-world practice–remain...
ABSTRACT To sense the outside world, some neurons protrude across epithelia, cellular barriers that line every surface of our bodies. study morphogenesis such neurons, we examined C. elegans amphid, in which dendrites through a glial channel at nose. During development, amphid extend by attaching to nose via DYF-7, type protein typically found epithelial apical ECM. Here, show and glia exhibit properties, including tight junctions apical-basal polarity, develop manner resembling other...
A fundamental step in regeneration is rapid growth to replace lost tissue. Cells must generate sufficient lipids, nucleotides, and proteins fuel cell division. To define metabolic pathways underlying regenerative growth, we undertake a multimodal investigation of reprogramming Xenopus tropicalis appendage regeneration. Regenerating tissues have increased glucose uptake; however, inhibition glycolysis does not decrease Instead, funneled the pentose phosphate pathway (PPP), which essential for...
Somatosensory neurons (SSNs) that detect and transduce mechanical, thermal, chemical stimuli densely innervate an animal’s skin. However, although epidermal cells provide the first point of contact for sensory stimuli. our understanding roles play in SSN function, particularly nociception, remains limited. Here, we show stimulating Drosophila elicits activation SSNs including nociceptors triggers a variety behavior outputs, avoidance escape. Further, find are intrinsically mechanosensitive...
Cancer drug development is currently limited by a paradigm of preclinical evaluation that does not adequately recapitulate the complexity intact human tumor microenvironment (TME). To overcome this, we combined trackable intratumor microdosing (CIVO) with spatial biology readouts to directly assess effects in patient tumors situ.In first-of-its-kind phase 0 clinical trial, explored an investigational stage SUMOylation-activating enzyme (SAE) inhibitor, subasumstat (TAK-981) 12 patients head...
In insects, a family of peptides with sequence homology to the vertebrate calcitonins has been implicated in control diuresis, process that includes mixing hemolymph. Here, we show member insect calcitonin-like diuretic hormone (CLDH) is present American lobster, Homarus americanus, serving, at least part, as powerful modulator cardiac output. Specifically, during an ongoing EST project, transcript encoding putative H. americanus CLDH precursor was identified; full-length cDNA subsequently...
Dendrites exhibit enormous diversity in form and can differ size by several orders of magnitude even a single animal. However, whether neurons with large dendrite arbors have specialized mechanisms to support their growth demands is unknown. To address this question, we conducted genetic screen for mutations that differentially affected different-sized arbors. From screen, identified mutant selectively affects without affecting small or the animal overall. This disrupts putative amino acid...
Melanoma clinical outcomes emerge from incompletely understood genetic mechanisms operating within the tumor and its microenvironment. Here, we used single-cell RNA-based spatial molecular imaging (RNA-SMI) in patient-derived archival tumors to reveal clinically relevant markers of malignancy progression prognosis. We examined gene expression 203,472 cells inside benign malignant melanocytic neoplasms, including nevi primary invasive metastatic melanomas. Algorithmic cell clustering paired...
The pathophysiology of silicosis is poorly understood, limiting development therapies for those who have been exposed to the respirable particle. We explored mechanisms silica-induced pulmonary fibrosis in human lung samples collected from patients with occupational exposure silica and a longitudinal mouse model using multiple modalities including whole-lung single-cell RNA sequencing histological, biochemical, physiologic assessments. In addition inflammation fibrosis, intratracheal...
Chemotherapy induced peripheral neuropathy (CIPN), a side effect of many anti-cancer drugs including the vinca alkaloids, is characterized by severe pain syndrome that compromises treatment in patients. Currently there are no effective treatments for this except reduction drug dose. Existing data supports model associated with CIPN result augmenting function sensory nociceptors but cellular mechanisms underlying effects on neuron not well described. Studies from animal models have suggested...
I give an account of disquieting experiences in relation to my treatment and those with chronic illness the acute system during 5 years' experience as informal caregiver. People (and their caregivers), particularly stroke survivors face a biased, frustrating even dangerous care hospital where they are second class. Well-being stability from rehabilitation self-management can be undermined. Dow McDonald's (2007) concept: 'disenfranchised contractors' within early discharge 'hospital home'...
Abstract The pathophysiology of silicosis is poorly understood, limiting development therapies for those who have been exposed to the respirable particle. We explored mechanisms silica-induced pulmonary fibrosis in a mouse model using multiple modalities including wholelung single-nucleus RNA sequencing. These analyses revealed that addition inflammation and fibrosis, intratracheal silica challenge induced osteoclast-like differentiation alveolar macrophages recruited monocytes, driven by...
Latency remains a major obstacle to finding cure for HIV despite the availability of antiretroviral therapy. Due virus dormancy, limited biomarkers are available identify latent HIV-infected cells. Profiling individual cells is needed explore potential latency and study mechanisms persistence that maintain reservoir.