A5001407509- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Olfactory and Sensory Function Studies
- Cognitive Abilities and Testing
- Photoreceptor and optogenetics research
- Stress Responses and Cortisol
- CRISPR and Genetic Engineering
- Memory and Neural Mechanisms
- Planarian Biology and Electrostimulation
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Protist diversity and phylogeny
- Cell Image Analysis Techniques
- Barrier Structure and Function Studies
- Arctic and Russian Policy Studies
- Gastrointestinal motility and disorders
- International Environmental Law and Policies
- Photochromic and Fluorescence Chemistry
- Genetic and Kidney Cyst Diseases
- Congenital heart defects research
- Family Dynamics and Relationships
- Cellular Mechanics and Interactions
- Gender, Labor, and Family Dynamics
- Developmental Biology and Gene Regulation
- International Maritime Law Issues
Massachusetts Institute of Technology
2018-2022
Boston VA Research Institute
2019-2020
Harvard University
2013-2020
Boston Children's Hospital
2013-2020
University of Michigan
2007-2011
Inimex Pharmaceuticals (Canada)
2000
Animals must weigh competing needs and states to generate adaptive behavioral responses the environment. Sensorimotor circuits are thus tasked with integrating diverse external internal cues relevant these context-appropriate behaviors. However, mechanisms that underlie this integration largely unknown. Here, we show a wide range of stimuli converge upon single Caenorhabditis elegans olfactory neuron modulate food-seeking behavior. Using an unbiased ribotagging approach, find expression...
The basolateral complex of the amygdala (BLA) is critical for acquisition and expression Pavlovian fear conditioning in rats. Nonetheless, rats with neurotoxic BLA lesions can acquire conditional after overtraining (75 trials). capacity to memory may be mediated by central nucleus (CEA). To examine this issue, we examined influence CEA or reversible inactivation on freezing Rats pretraining (whether alone combination lesions) did not either context an auditory stimulus extensive...
ABSTRACT To sense the outside world, some neurons protrude across epithelia, cellular barriers that line every surface of our bodies. study morphogenesis such neurons, we examined C. elegans amphid, in which dendrites through a glial channel at nose. During development, amphid extend by attaching to nose via DYF-7, type protein typically found epithelial apical ECM. Here, show and glia exhibit properties, including tight junctions apical-basal polarity, develop manner resembling other...
Dendrites and axons are two major neuronal compartments with differences that critical for functions. To learn about the differential regulation of dendritic axonal development, we conducted a genetic screen in Drosophila isolated arbor reduction 1 (dar1) mutants, which display defects but not growth. The dar1 gene encodes novel transcription regulator Krüppel-like factor family. Neurons lacking function have severely reduced growth microtubule- F-actin-based branches. In contrast,...
Abstract Developmental experiences play critical roles in shaping adult physiology and behavior. We others previously showed that Caenorhabditiselegans which transiently experienced dauer arrest during development (postdauer) exhibit distinct gene expression profiles as compared to control adults bypassed the stage. In particular, patterns of subsets chemoreceptor genes are markedly altered postdauer adults. Whether levels drive behavioral plasticity is unknown. Here, we show enhanced...
Dendrites develop elaborate morphologies in concert with surrounding glia, but the molecules that coordinate dendrite and glial morphogenesis are mostly unknown. C. elegans offers a powerful model for identifying such factors. Previous work this system examined dendrites glia within epithelia, similar to mammalian sense organs. Here, we focus on neurons BAG URX, which not part of an epithelium instead form membranous attachments single cell at nose, reminiscent dendrite-glia contacts brain....
Neurons develop elaborate morphologies that provide a model for understanding cellular architecture. By studying C. elegans sensory dendrites, we previously identified genes act to promote the extension of ciliated dendrites during embryogenesis. Interestingly, nonciliated dendrite oxygen-sensing neuron URX is not affected by these genes, suggesting it develops through distinct mechanism. Here, use visual forward genetic screen identify mutants affect morphogenesis. We find disruption MAP...
ABSTRACT Animals must weigh competing needs and states to generate adaptive behavioral responses the environment. Sensorimotor circuits are thus tasked with integrating diverse external internal cues relevant these context-appropriate behaviors. However, mechanisms that underlie this integration largely unknown. Here, we show a wide range of stimuli converge upon single C. elegans olfactory neuron modulate food-seeking behavior. Using an unbiased ribotagging approach, find expression...
Cell type-specific molecular profiling is widely used to gain new insights into the diverse cell types that make up complex biological tissues. Translating ribosome affinity purification (TRAP) a method in which expression of epitope-tagged ribosomal subunits allows one purify actively translating mRNAs without need for sorting or fixation. We adapted this use C. elegans identify novel transcripts single effects environmental changes on transcriptomes larger cohorts cells. In protocol, we...
ABSTRACT To sense the outside world, some neurons protrude across epithelia, cellular barriers that line every surface of our bodies. study morphogenesis such neurons, we examined C. elegans amphid, in which dendrites through a glial channel at nose. During development, amphid extend by attaching to nose via DYF-7, type protein typically found epithelial apical ECM. Here, show and glia exhibit properties, including tight junctions apical-basal polarity, develop manner resembling other...
ABSTRACT Dendrites develop elaborate morphologies in concert with surrounding glia, but the molecules that coordinate dendrite and glial morphogenesis are mostly unknown. C. elegans offers a powerful model for identifying such factors. Previous work this system examined dendrites glia within epithelia, similar to mammalian sense organs. Here, we focus on neurons BAG URX, which not part of an epithelium instead form membranous attachments single cell at nose, reminiscent dendrite-glia...
ABSTRACT Neurons develop elaborate morphologies that provide a model for understanding cellular architecture. By studying C. elegans sensory dendrites, we previously identified genes act to promote the extension of ciliated dendrites during embryogenesis. Interestingly, nonciliated dendrite oxygen-sensing neuron URX is not affected by these genes, suggesting it develops through distinct mechanism. Here, use visual forward genetic screen identify mutants affect morphogenesis. We find...