A5073322208- Immune Cell Function and Interaction
- Eosinophilic Disorders and Syndromes
- Tuberous Sclerosis Complex Research
- Tea Polyphenols and Effects
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Pediatric health and respiratory diseases
- Histiocytic Disorders and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Biomarkers in Disease Mechanisms
- Nephrotoxicity and Medicinal Plants
- Occupational and environmental lung diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Bone and Dental Protein Studies
- Extracellular vesicles in disease
- Vanadium and Halogenation Chemistry
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Respiratory viral infections research
- Histone Deacetylase Inhibitors Research
- Single-cell and spatial transcriptomics
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cytomegalovirus and herpesvirus research
- Burn Injury Management and Outcomes
- bioluminescence and chemiluminescence research
University of Cincinnati
2005-2024
University of Cincinnati Medical Center
2016-2024
Pulmonary and Critical Care Associates
2021
SleepMed
2021
Shriners Hospitals for Children - Cincinnati
2009-2012
Shriners Hospitals for Children - Erie
2007-2012
Shriners Hospitals for Children
2007
Heavy metal tungsten alloys have replaced lead and depleted uranium in many munitions applications, due to public perception of these elements as environmentally unsafe. Tungsten materials left the environment may become bioaccessible tungstate, which might population exposure through water soil contamination. Although had been considered a relatively inert toxicologically safe material, recent research findings raised concerns about possible deleterious health effects after acute chronic...
Recent publications have demonstrated that human resident and inflammatory monocyte (IM) subpopulations equivalents in rodents. The effect of thermal injury upon these has not been studied. Mice were given a scald burn killed on postburn days (PBDs) 2, 4, 8. Bone marrow, blood, spleen white cells isolated, the percentage monocytes (CD11b LY6C), IMs progenitors (macrophage-colony-forming unit [M-CFU]) determined. ability each population to make TNF-alpha was determined by intracellular...
Abstract Chronic obstructive pulmonary disease (COPD) is a devastating with no effective therapies. We investigated the role of C-type lectin receptor, CLEC5A, in macrophage activation and pathogenesis mouse model COPD. demonstrate that CLEC5A expressed on alveolar macrophages mice exposed long-term to cigarette smoke (CS), as well human smokers. also show CLEC5A-mediated enhanced cytokine elaboration alone, combination LPS or GM-CSF CS-exposed mice. Furthermore, using Clec5a-deficient mice,...
Pulmonary Langerhans cell histiocytosis (PLCH) is a rare smoking-related lung disease characterized by dendritic (DC) accumulation, bronchiolocentric nodule formation, and cystic remodeling. Approximately 50% of patients with PLCH harbor somatic BRAF-V600E mutations in cells the myeloid/monocyte lineage. However, rarity lack animal models have impeded study pathogenesis. Here, we establish cigarette smoke–exposed (CS-exposed) BRAF-V600E–mutant mouse model that recapitulates many hallmark...
The potential for adverse health effects of using tungsten and its alloys in military munitions are an important concern to both civilians the US military. toxicological implications exposure tungsten, alloys, soluble tungstate (Na2WO4) currently under investigation. To examine toxicity, a series experiments determine vitro on cells immune system were performed. We identified alterations isolated human peripheral blood lymphocytes (PBL) treated with sodium (0.01, 0.1, 1.0, 10 mM). Analyses...
Lymphangioleiomyomatosis (LAM) is a rare lung disease of women that leads to progressive cyst formation and accelerated loss pulmonary function. Neoplastic smooth muscle cells from an unknown source metastasize the drive destructive remodeling. Given role NK in immune surveillance, we postulated cell activating receptors their cognate ligands are involved LAM pathogenesis. We found for NKG2D receptor UL-16 binding protein 2 (ULBP2) ULBP3 localized cystic lesions nodules. elevated soluble...
Abstract Chronic Obstructive Pulmonary Disease (COPD) is the third leading cause of death worldwide. COPD frequently punctuated by acute exacerbations that are precipitated primarily infections, which increase both morbidity and mortality inflates healthcare costs. Despite significance exacerbations, little understanding immune function in exists. Natural killer (NK) cells important effectors innate adaptive responses to pathogens NK cell altered smokers COPD. Using high-dimensional flow...
Previously, we have shown that statistical synergism between amino acid variants in thyroglobulin (Tg) and specific HLA-DR3 pocket sequence signatures conferred a high risk for autoimmune thyroid disease (AITD). Therefore, hypothesized this mirrors biochemical interaction Tg peptides HLA-DR3, which is key to the pathoetiology of AITD. To test hypothesis, designed recombinant expression system was used express HLA-DR molecules harboring either AITD susceptibility or resistance DR sequences....
Immature myeloid cells have been implicated as a source of postburn inflammation, and the appearance these correlates with enhanced upregulation hematopoiesis. The role proliferative in immune changes has not directly tested. Gemcitabine, ribonucleotide reductase inhibitor, shown to deplete immature tumor models while sparing mature cells, leading restored lymphocyte function regression. We treated burn mice at day 6 (PBD6) 120 mg/kg gemcitabine. On PBD8, splenocytes were taken stimulated...
The pathophysiology of silicosis is poorly understood, limiting development therapies for those who have been exposed to the respirable particle. We explored mechanisms silica-induced pulmonary fibrosis in human lung samples collected from patients with occupational exposure silica and a longitudinal mouse model using multiple modalities including whole-lung single-cell RNA sequencing histological, biochemical, physiologic assessments. In addition inflammation fibrosis, intratracheal...
Tuberous sclerosis complex (TSC) is a tumor-suppressor syndrome affecting multiple organs, including the brain, skin, kidneys, heart, and lungs. TSC associated with mutations in TSC1 or TSC2, resulting hyperactivation of mTOR 1 (mTORC1). Clinical trials demonstrate that mTORC1 inhibitors decrease tumor volume stabilize lung function patients; however, are cytostatic not cytocidal, long-term benefits toxicities uncertain. Previously, we identified rapamycin-insensitive upregulation...
Abstract The pathophysiology of silicosis is poorly understood, limiting development therapies for those who have been exposed to the respirable particle. We explored mechanisms silica-induced pulmonary fibrosis in a mouse model using multiple modalities including wholelung single-nucleus RNA sequencing. These analyses revealed that addition inflammation and fibrosis, intratracheal silica challenge induced osteoclast-like differentiation alveolar macrophages recruited monocytes, driven by...
Chronic obstructive disease (COPD) risk factors, smoking, and chronic infection (cytomegalovirus [CMV]) may mold natural killer (NK) cell populations. What is not known the magnitude of effect CMV seropositivity imparts on populations smokers with at for COPD. We investigate independent influence NK differential effects when stratifying by COPD degree smoking history.Descriptive statistics determine relationship between cytotoxic demographic clinical variables. Multivariable linear...
Tungstate (WO²⁻₄) has been identified as a ground water contaminant at military firing ranges and can be absorbed by ingestion. In this study, C57BL6 mice were exposed to sodium tungstate (Na2WO4·2H2O) (0, 2, 62.5, 125, 200 mg/kg/day) in their drinking for an initial 28-day screen one-generation (one-gen) model. Twenty-four hours prior euthanasia, intraperitoneally injected with Staphylococcal enterotoxin B (SEB) (20 μg/mouse) or saline controls. After splenocytes blood collected stained...
Respiratory syncytial virus (RSV) is a common cause of respiratory tract infection in vulnerable populations. Natural killer (NK) cells and dendritic (DC) are important for the effector functions both cell types following infection. Wild-type NKG2D-deficient mice were infected with RSV. Lung pathology was assessed by histology. Dendritic function phenotype evaluated enzyme-linked immunosorbent assay flow cytometry. The expression NKG2D ligands on lung lymph node DCs measured immunostaining...
Cytomegalovirus (CMV) represents an understudied chronic infection, usually contracted early in life, that causes immune system alterations which may contribute to airflow limitations a cohort of veterans with high prevalence smoking. We studied 172 participants at-risk for and limitation available CMV serology assess the relationship between infection obstructive pulmonary disease (COPD)-related outcomes.The study includes who are smokers or at risk development COPD. Clinical data were...
In this study, we investigated the effects of (-)-epigallocatechin-3-gallate (EGCG) on wound healing both in vitro and vivo with without infection.EGCG has antimicrobial properties could be useful as a topical agent to prevent and/or treat infections.Normal fibroblasts were isolated from dermis C57BL/6 mice cultured 0, 0.001 0.400 mg/ml EGCG.In assays demonstrated that migration, proliferation, apoptosis inhibited at EGCG concentrations 0.100-0.400mg/ml.Expression α-smooth muscle actin...
<b>Abstract ID 98921</b> <b>Poster Board 358</b> <b>Background:</b> PLCH occurs almost exclusively in cigarette smokers and has a median duration of survival from the time diagnosis 12.5 years. is characterized by bronchiolocentric histiocyte accumulation, inflammatory lesions, nodule formation, cystic remodeling. A causative link between acquired BRAF (kinase RAS/MAPK signaling pathway) mutations myeloid/monocyte lineage development neoplasms been reported, common, mutation (V600E) was...
<h3>Background</h3> Recurrent or metastatic head and neck squamous cell cancer (R/M HNSCC) has dismal survival rates. Immunotherapy revolutionized treatment, but response rates remain low. Treatment with metformin is associated decreased risk of HNSCC in diabetic patients. It been shown that increases CD8+ tumor infiltrating lymphocytes inhibits PD-L1 membrane localization. Furthermore, peripheral NK cells, which harbor PD-1. Therefore, we hypothesized combination therapy anti-PD-1 may...