A5031234698- Cancer Genomics and Diagnostics
- Radiomics and Machine Learning in Medical Imaging
- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- interferon and immune responses
- Nanoparticle-Based Drug Delivery
- Chemical Reactions and Isotopes
- Immune cells in cancer
- Cell Image Analysis Techniques
- Computational Drug Discovery Methods
- Biosimilars and Bioanalytical Methods
- Cancer, Hypoxia, and Metabolism
- Advanced Biosensing Techniques and Applications
- Pancreatic and Hepatic Oncology Research
- Cellular Mechanics and Interactions
- Cancer Immunotherapy and Biomarkers
- Monoclonal and Polyclonal Antibodies Research
- Ferroptosis and cancer prognosis
- Cytokine Signaling Pathways and Interactions
- Cancer Cells and Metastasis
- Microtubule and mitosis dynamics
- PI3K/AKT/mTOR signaling in cancer
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Immunotherapy and Immune Responses
Presage Biosciences (United States)
2023
Fred Hutch Cancer Center
2016-2023
Seattle University
2023
University of California, San Francisco
2011-2017
California Institute for Regenerative Medicine
2014
Indiana University Bloomington
2007-2010
Developing probes for the detection of reactive oxygen species (ROS), a hallmark many pathophysiological process, is imperative to both understanding precise roles ROS in life-threatening diseases and optimizing therapeutic interventions. We herein report an all-in-one fluorescent semiconducting polymer based far-red near-infrared (NIR) Pdot nanoprobe ratiometric hypochlorous acid (HOCl). The fabrication takes advantage flexible design by incorporating target-sensitive target-inert...
Extracellular matrix (ECM) stiffness induces focal adhesion assembly to drive malignant transformation and tumor metastasis. Nevertheless, how force alters adhesions promote progression remains unclear. Here, we explored the role of protein vinculin, a force-activated mechanotransducer, in mammary epithelial tissue invasion. We found that ECM stabilizes vinculin-talin-actin scaffolding complex facilitates PI3K-mediated phosphatidylinositol (3,4,5)-triphosphate phosphorylation. Using defined...
Metastasis requires tumor cells to navigate through a stiff stroma and squeeze confined microenvironments. Whether tumors exploit unique biophysical properties metastasize remains unclear. Data show that invading mammary cells, when cultured in stiffened three-dimensional extracellular matrix recapitulates the primary stroma, adopt basal-like phenotype. Metastatic exert higher integrin-mediated traction forces at bulk molecular levels, consistent with motor-clutch model which motors clutches...
Cancer-associated fibroblast (CAF) heterogeneity is increasingly appreciated, but the origins and functions of distinct CAF subtypes remain poorly understood. The abundant transcriptionally diverse population in pancreatic ductal adenocarcinoma (PDAC) thought to arise from a common cell origin, stellate cells (PSC), with diversification resulting cytokine growth factor gradients within tumor microenvironment. Here we analyzed differentiation function PSCs during progression vivo. Contrary...
The efficient selection and isolation of individual cells interest from a mixed population is desired in many biomedical clinical applications. Here we show the concept using photoswitchable semiconducting polymer dots (Pdots) as an optical 'painting' tool, which enables certain adherent based on their fluorescence, spatial morphological features, under microscope. We first develop Pdot that can switch between bright (ON) dark (OFF) states reversibly with 150-fold contrast ratio irradiation...
A perspective on abiotic material encapsulation inside virus capsids is provided. The emphasis the physical principles of assembly relevant to packaging, strategies for and capsid modification, emerging applications.
Bright long-wavelength-excitable semiconducting polymer dots (LWE-Pdots) are highly desirable for in vivo imaging and multiplexed vitro bioassays. LWE-Pdots have been obtained by incorporating a near-infrared (NIR) emitter into the backbone of host to develop binary donor-acceptor (D-A) system. However, they usually suffer from severe concentration quenching trade-off between fluorescence quantum yield (Φf ) absorption cross-section (σ). Herein, we describe ternary component (D1 /D2 -A)...
DNA damage repair (DDR) is a double-edged sword with different roles in cancer susceptibility and drug resistance. Recent studies suggest that DDR inhibitors affect immune surveillance. However, this phenomenon poorly understood. We report methyltransferase SMYD2 plays an essential role nonhomologous end joining (NHEJ), driving tumor cells adaptive to radiotherapy. Mechanically, response damage, mobilized onto chromatin methylates Ku70 at lysine-74, lysine-516, lysine-539, leading increased...
Cancer drug development is currently limited by a paradigm of preclinical evaluation that does not adequately recapitulate the complexity intact human tumor microenvironment (TME). To overcome this, we combined trackable intratumor microdosing (CIVO) with spatial biology readouts to directly assess effects in patient tumors situ.In first-of-its-kind phase 0 clinical trial, explored an investigational stage SUMOylation-activating enzyme (SAE) inhibitor, subasumstat (TAK-981) 12 patients head...
Optical nonlinear properties of cusps formed at the junction between two circular apertures in a metal film have been studied by scanning confocal microscopy. For gold, both second harmonic and broadband emission are enhanced when pump polarization is directed across gap cusps, similar to behavior recently bowtie antennas apertures. However, field enhancements also present perpendicular direction. A quantitative explanation observed relative signal intensities requires considering...
Cell surface receptors are central to the cell's ability generate coordinated responses multitude of biochemical and physical cues in microenvironment. However, mechanisms by which enable this concerted cellular response remain unclear. To investigate effect tension on cell receptors, we combined novel high-resolution imaging single particle tracking with established assays examine TGFβ signaling. We find that discretely organized segregated spatial domains at surface. Integrin-rich focal...
Pancreatic ductal adenocarcinoma (PDA) is characterized by a pronounced fibroinflammatory stromal reaction consisting of inordinate levels hyaluronan (HA), collagen, immune cells, and activated fibroblasts that work in concert to generate robust physical barrier the perfusion diffusion small molecule therapeutics. The targeted depletion with PEGylated recombinant human hyaluronidase (PEGPH20) lowers interstitial gel–fluid pressures re-expands collapsed intratumoral vasculature, improving...
We present a novel platform for superresolution imaging of adhesion proteins on physiologically relevant substrates.
Abstract Bright long‐wavelength‐excitable semiconducting polymer dots (LWE‐Pdots) are highly desirable for in vivo imaging and multiplexed vitro bioassays. LWE‐Pdots have been obtained by incorporating a near‐infrared (NIR) emitter into the backbone of host to develop binary donor–acceptor (D–A) system. However, they usually suffer from severe concentration quenching trade‐off between fluorescence quantum yield (Φ f ) absorption cross‐section (σ). Herein, we describe ternary component (D 1...
<p>Subasumstatinduces IFNβ in immune cells.</p>