A5084605921- RNA modifications and cancer
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Protein Degradation and Inhibitors
- Plant Molecular Biology Research
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
- Cancer-related gene regulation
- Erythrocyte Function and Pathophysiology
- Advanced Proteomics Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- Bioinformatics and Genomic Networks
- S100 Proteins and Annexins
- RNA regulation and disease
- Advanced Breast Cancer Therapies
- Lipid Membrane Structure and Behavior
- Chronic Myeloid Leukemia Treatments
- Epigenetics and DNA Methylation
- Nerve injury and regeneration
- Hippo pathway signaling and YAP/TAZ
- CAR-T cell therapy research
- Cell Adhesion Molecules Research
- Biotin and Related Studies
University of California, San Francisco
2016-2025
University of San Francisco
2023
UCSF Helen Diller Family Comprehensive Cancer Center
2013-2023
Boston Children's Hospital
2022
Harvard University
2022
City College of San Francisco
2011-2015
IONICS Mass Spectrometry (Canada)
2015
Universidad de Navarra
1997-2008
Institute of Botany
2008
Chinese Academy of Sciences
2008
Brassinosteroids (BRs) bind to the extracellular domain of receptor kinase BRI1 activate a signal transduction cascade that regulates nuclear gene expression and plant development. Many components BR signaling pathway have been identified studied in detail. However, substrate transduces downstream remains unknown. Proteomic studies plasma membrane proteins lead identification three homologous BR-signaling kinases (BSK1, BSK2, BSK3). The BSKs are phosphorylated by vitro interact with vivo....
Adoption of targeted mass spectrometry (MS) approaches such as multiple reaction monitoring (MRM) to study biological and biomedical questions is well underway in the proteomics community. Successful application depends on ability generate reliable assays that uniquely confidently identify target peptides a sample. Unfortunately, there wide range criteria being applied say an assay has been successfully developed. There no consensus what are acceptable little understanding impact variable...
How is protein synthesis initiated locally in neurons? We found that mTOR (mechanistic target of rapamycin) was activated and then up-regulated injured axons, owing to local translation messenger RNA (mRNA). This mRNA transported into axons by the cell size-regulating RNA-binding nucleolin. Furthermore, controlled axons. included regulation its own retrograde injury signaling molecules such as importin β1 STAT3 (signal transducer activator transcription 3). Deletion 3' untranslated region...
The plant steroid hormones brassinosteroids (BRs) play an important role in a wide range of developmental and physiological processes. How BR signaling regulates diverse processes remains unclear. To understand the molecular details responses, we performed proteomics study BR-regulated proteins Arabidopsis using two-dimensional DIGE coupled with LC-MS/MS. We identified 42 proteins, which are predicted to potential roles regulation specific cellular processes, such as signaling, cytoskeleton...
The Ras/mitogen-activated protein kinase (MAPK) pathway plays a critical role in transducing mitogenic signals from receptor tyrosine kinases. Loss-of-function mutations one feedback regulator of Ras/MAPK signaling, SPRED1 (Sprouty-related with an EVH1 domain), cause Legius syndrome, autosomal dominant human disorder that resembles Neurofibromatosis-1 (NF1). Spred1 functions as negative the pathway; however, underlying molecular mechanism is poorly understood. Here we show neurofibromin, NF1...
Signal transduction involves posttranslational modifications and protein-protein interactions, which can be studied by proteomics. In Arabidopsis, the steroid hormone (brassinosteroid (BR)) binds to extracellular domain of a receptor kinase (BRI1) initiate phosphorylation/dephosphorylation cascade that controls gene expression plant growth. Here we detected early BR signaling events identified response proteins using prefractionation two-dimensional (2-D) DIGE. Proteomic changes induced...
Clustered Kv1 K + channels regulate neuronal excitability at juxtaparanodes of myelinated axons, axon initial segments, and cerebellar basket cell terminals (BCTs). These are part a larger protein complex that includes adhesion molecules scaffolding proteins. To identify proteins assembly, clustering, and/or maintenance axonal channel complexes, we immunoprecipitated Kv1.2 α subunits, then used mass spectrometry to interacting We found disintegrin metalloproteinase 22 (ADAM22) is component...
Abnormalities in dendritic spines are manifestations of several neurodevelopmental and psychiatric diseases. TAOK2 is one the genes 16p11.2 locus, copy number variations which associated with autism schizophrenia. Here, we show that kinase activity serine/threonine encoded by required for spine maturation. depletion results unstable protrusions, mislocalized shaft-synapses, loss compartmentalization NMDA receptor-mediated calcium influx. Using chemical-genetics mass spectrometry, identified...
Axon initial segments (AISs) generate action potentials and regulate the polarized distribution of proteins, lipids, organelles in neurons. While mechanisms AIS Na+ K+ channel clustering are understood, molecular that stabilize control neuronal polarity remain obscure. Here, we use proximity biotinylation mass spectrometry to identify proteome. We target biotin-ligase BirA* by generating fusion proteins with NF186, Ndel1, Trim46; these chimeras map organization intracellular membrane,...
Spectrins form a submembranous cytoskeleton proposed to confer strength and flexibility neurons participate in ion channel clustering at axon initial segments (AIS) nodes of Ranvier. Neuronal spectrin cytoskeletons consist diverse β subunits αII spectrin. Although is found both axonal somatodendritic domains, using proteomics, biochemistry, superresolution microscopy, we show that βIV interact periodic AIS cytoskeleton. To determine the role spectrins nervous system, generated Sptan1 f/f...