A5100628097- Cancer Genomics and Diagnostics
- Prostate Cancer Treatment and Research
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Lung Cancer Treatments and Mutations
- Advanced Proteomics Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- PARP inhibition in cancer therapy
- Radiopharmaceutical Chemistry and Applications
- Radiomics and Machine Learning in Medical Imaging
- 3D Printing in Biomedical Research
- Genetics, Bioinformatics, and Biomedical Research
- Blood properties and coagulation
- Nuclear Structure and Function
- Genetic factors in colorectal cancer
- RNA modifications and cancer
- Colorectal Cancer Treatments and Studies
- Nanoparticle-Based Drug Delivery
- Ferroptosis and cancer prognosis
- Health Systems, Economic Evaluations, Quality of Life
- Economic and Financial Impacts of Cancer
- Pluripotent Stem Cells Research
- RNA Research and Splicing
University of Southern California
2018-2025
Larry Ellison Foundation
2020-2024
Ellison Institute of Technology
2019-2024
Material Sciences (United States)
2021-2024
Convergent Science (United States)
2023
Epic Sciences (United States)
2015-2022
Uniformed Services University of the Health Sciences
2020-2022
Henry M. Jackson Foundation
2019-2022
Weill Cornell Medicine
2021
Southern California University for Professional Studies
2021
Adoption of targeted mass spectrometry (MS) approaches such as multiple reaction monitoring (MRM) to study biological and biomedical questions is well underway in the proteomics community. Successful application depends on ability generate reliable assays that uniquely confidently identify target peptides a sample. Unfortunately, there wide range criteria being applied say an assay has been successfully developed. There no consensus what are acceptable little understanding impact variable...
The microstructure of the nucleus, one most studied but least understood cellular organelles, is subject much debate. Through use particle nanotracking, we detect and quantify micro-organization as well viscoelastic properties intranuclear region in single, live, interphase somatic cells. We find that stiffer than cytoplasm; it also more elastic viscous, which reveals displays an unexpectedly strong solid-like behavior. mean shear viscosity elasticity Swiss 3T3 fibroblasts are 520 Poise (P)...
Nanoparticles have been investigated as drug delivery vehicles, contrast agents, and multifunctional devices for patient care. Current nanoparticle-based therapeutic strategies cancer treatment are mainly based on of chemotherapeutic agents to induce apoptosis or DNA/siRNA regulate oncogene expression. Here, a nanoparticle system that demonstrates an alternative approach the cancers through inhibition cell invasion, while serving magnetic resonance optical imaging agent, is presented. The...
Abstract Following uncontrolled proliferation, a subset of primary tumour cells acquires additional traits/mutations to trigger phenotypic changes that enhance migration and are hypothesized be the initiators metastasis. This study reveals an adaptive mechanism harnesses synergistic paracrine signalling via IL-6/8, which is amplified by cell proliferation density, directly promote migration. effect occurs in metastatic human sarcoma carcinoma cells– but not normal or non-metastatic cancer...
Abstract The heterogeneity of an individual patient's tumor has been linked to treatment resistance, but quantitative biomarkers rapidly and reproducibly evaluate in a clinical setting are currently lacking. Using established tools available College American Pathologists–accredited Clinical Laboratory Improvement Amendments–certified laboratory, we quantified digital pathology features on 9,225 circulating cells (CTC) from 179 unique metastatic castration-resistant prostate cancer (mCRPC)...
We present a deep proteogenomic profiling study of 87 lung adenocarcinoma (LUAD) tumors from the United States, integrating whole-genome sequencing, transcriptome proteomics and phosphoproteomics by mass spectrometry, reverse-phase protein arrays. identify three subtypes somatic genome signature analysis, including transition-high subtype enriched with never smokers, transversion-high current structurally altered former TP53 alterations, genome-wide structural alterations. show that...
Importance Esophageal cancer (EC) is the 7th most common worldwide and 14th in US. More data are needed to study changing incidence patterns of its 2 primary histologic subtypes, squamous cell carcinoma esophagus (SCE) adenocarcinoma (ACE). Objective To examine temporal trends rates EC, ACE, SCE from 1975 through 2018. Design, Setting, Participants In this population-based cross-sectional study, were derived 9 Surveillance, Epidemiology, End Results (SEER) registries January December 2018...
The Affymetrix GeneChip platform was used to build a gene expression database of the normal human body. Postmortem tissues represent valuable source biological materials for this type study, but their use entails some delays before harvesting such tissues. We first evaluated RNA quality obtained from 3-5 h postmortem and found variations that were both tissue donor-dependent. RNAs extracted brain regions higher than those gut, while cause death significant factor in donor-dependent...
Cells in vivo are constantly subjected to mechanical shear stresses that play important regulatory roles various physiological and pathological processes. Cytoskeletal reorganizations occur response flow have been studied extensively, but whether the cytoplasm of an adherent cell adapts its properties respond is largely unknown. Here we develop a new method where fluorescent nanoparticles ballistically injected into cells probe, with high resolution, possible local viscoelastic changes...
Abstract In vitro models that accurately mimic the microenvironment of invading glioblastoma multiform (GBM) cells will provide a high‐throughput system for testing potential anti‐invasion therapies. Here, ability chitosan‐polycaprolactone polyblend nanofibers to promote migratory phenotype in human GBM by altering nanotopography nanofiber membranes is investigated. Fibers are prepared with diameters 200 nm, 400 and 1.1 μm, either randomly oriented or aligned produce six distinct...
Abstract Liquid biopsy, particularly the analysis of circulating tumor DNA (ctDNA), has demonstrated considerable promise for numerous clinical intended uses. Successful validation and commercialization novel ctDNA tests have potential to improve outcomes patients with cancer. The goal Blood Profiling Atlas Consortium (BloodPAC) is accelerate development liquid biopsy assays that will be introduced into clinic. To accomplish this goal, BloodPAC conducts research in following areas: Data...
Abstract Purpose: Aggressive variant prostate cancer (AVPC) represents a clinical subset distinguished by therapy resistance and poor prognosis, linked to combined losses of the tumor suppressor genes (TSG) PTEN, RB1, TP53. Circulating cells (CTC) provide minimally invasive opportunity for identification molecular characterization AVPC. We aimed evaluate incidence significance compound (2+)TSG genomic instability in CTC, expand set biomarkers relevant Experimental Design: Genomic analysis...
Both tumors and aging alter the immune landscape of tissues. These interactions may play an important role in tumor progression among elderly patients suggest considerations for patient care. We leverage large-scale genomic clinical databases to perform comprehensive comparative analysis molecular cellular markers checkpoint blockade (ICB) response with age. analyses demonstrate that is associated increased mutational burden, expression decreased promoter methylation genes, interferon gamma...
Nucleus movement is essential during nucleus positioning for tissue growth and development in eukaryotic cells. However, molecular regulators of interphase fibroblasts have yet to be identified. Here, we report that nuclei Swiss 3T3 undergo enhanced when subjected shear flows. Such includes both rotation translocation dependent on microtubule, not F-actin, structure. Through inactivation Rho GTPases, well-known mediators cytoskeleton reorganization, demonstrate Cdc42, RhoA or Rac1, controls...