A5077852290- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Prostate Cancer Treatment and Research
- Bone Metabolism and Diseases
- Bone health and treatments
- Single-cell and spatial transcriptomics
- Multiple Myeloma Research and Treatments
- Proteoglycans and glycosaminoglycans research
- Medical Imaging and Pathology Studies
- Extracellular vesicles in disease
- Lung Cancer Treatments and Mutations
- Meat and Animal Product Quality
- Cancer, Lipids, and Metabolism
- Membrane-based Ion Separation Techniques
- Membrane Separation Technologies
- Microfluidic and Bio-sensing Technologies
- Hepatocellular Carcinoma Treatment and Prognosis
- Molecular Biology Techniques and Applications
- Cell Adhesion Molecules Research
- Lung Cancer Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Protease and Inhibitor Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Lymphatic System and Diseases
- Tissue Engineering and Regenerative Medicine
University of Southern California
2015-2024
Convergent Science (United States)
2017-2023
Esteve (Spain)
2020
Universidad de Huelva
2020
Public Health Department
2020
Hospital General Universitario de Elche
2016
Scripps Research Institute
2012-2015
Inserm
2008-2011
Nantes Université
2007-2011
Universidad de Valladolid
2003-2006
Clinical studies have demonstrated that circulating tumor cells (CTCs) are present in the blood of cancer patients with known metastatic disease across major types epithelial malignancies. Recent shown concentration CTCs is prognostic overall survival breast, prostate, colorectal, and non-small cell lung cancer. This study characterizes identified using high-definition (HD)-CTC assay an ovarian patient stage IIIC disease. We characterized physical properties 31 HD-CTCs 50 normal leukocytes...
Molecular analysis of circulating and disseminated tumor cells (CTCs/DTCs) has great potential as a means for continuous evaluation prognosis treatment efficacy in near-real time through minimally invasive liquid biopsies. To realize this potential, however, methods molecular these rare must be developed validated. Here, we describe the integration imaging mass cytometry (IMC) using metal-labeled antibodies implemented on Fluidigm Hyperion Imaging System into workflow previously established...
Abstract Purpose: Aggressive variant prostate cancer (AVPC) represents a clinical subset distinguished by therapy resistance and poor prognosis, linked to combined losses of the tumor suppressor genes (TSG) PTEN, RB1, TP53. Circulating cells (CTC) provide minimally invasive opportunity for identification molecular characterization AVPC. We aimed evaluate incidence significance compound (2+)TSG genomic instability in CTC, expand set biomarkers relevant Experimental Design: Genomic analysis...
Liquid biopsies hold potential as minimally invasive sources of tumor biomarkers for diagnosis, prognosis, therapy prediction or disease monitoring. We present an approach parallel single-object identification circulating cells (CTCs) and tumor-derived large extracellular vesicles (LEVs) based on automated high-resolution immunofluorescence followed by downstream multiplexed protein profiling. Identification LEVs >6 µm in size CTC enumeration was highly correlated, with being 1.9 times...
Purpose. Circulating melanoma cells (CMCs) constitute a potentially important representation of time-resolved tumor biology in patients. To date, genomic characterization CMCs has been limited due to the lack robust methodology capable identifying them format suitable for downstream characterization. Here, we have developed detect intact that enables phenotypic, morphometric and analysis at single cell level. Experimental design. Blood samples from 40 metastatic patients 10 normal blood...
Currently, clinical characterization of metastatic breast cancer is based on tissue samples taken at time diagnosis. However, biopsies are invasive and tumors continuously evolving, which indicates the need for minimally longitudinal assessment tumor. Blood-based liquid provide minimal means serial sampling over course treatment opportunity to adjust therapies molecular markers. Here, we aim identify cellular changes that occur in lifespan an affected patient through single-cell proteomic...
Tumor heterogeneity is prevalent in both treatment-naïve and end-stage metastatic castration-resistant prostate cancer (PCa), may contribute to the broad range of clinical presentation, treatment response, disease progression. To characterize molecular associated with de novo PCa, multiplatform single cell profiling was performed using high definition analysis (HD-SCA). HD-SCA enabled morphoproteomic morphogenomic cells from touch preparations tissue cores (prostate bone marrow biopsies) as...
Liquid biopsy allows assessment of multiple analytes, providing temporal information with potential for improving understanding cancer evolution and clinical management patients. Although liquid biopsies are intensely investigated prediction response monitoring, preanalytic variables primary concern implementation, including categories collection method sample storage. Herein, an integrated high-density single-cell assay workflow morphometric genomic analysis the is used to characterize...
Breast cancer (BC) affects 1 in every 8 women the United States and is currently most prevalent worldwide. Precise staging at diagnosis prognosis are essential components for clinical management of BC patients. In this study, we set out to evaluate feasibility high-definition single cell (HDSCA) liquid biopsy (LBx) platform stratify late-stage BC, early-stage normal donors using peripheral blood samples. Utilizing 5 biomarkers, identified rare circulating events with epithelial, mesenchymal,...
The growth and differentiation of bone cells is controlled by various factors, which can be modulated heparan sulfates. Here, we investigated the effects an oversulfated exopolysaccharide (OS-EPS) on bone. We compared effect this compound with that a native EPS. Long-term administration OS-EPS causes cancellous loss in mice due, part, to increase number osteoclasts lining trabecular surface. No significant difference volume was found between EPS-treated age-matched control mice, underlying...
Bi-directional crosstalk between the tumor and microenvironment (TME) has been shown to increase rate of evolution play a key role in neoplastic progression, therapeutic resistance, patient's overall survival. Here, we set out use comprehensive liquid-biopsy analysis study cancer specific TME cells circulation their association with disease status. Cytokeratin+, CD45- circulating rare (CRCs) from nine breast four prostate patients were characterized through morphometrics, single-cell copy...
Delayed xenograft rejection (DXR) of pig organs by baboons currently represents the major obstacle to successful xenotransplantation. Although antibodies (Abs) are believed play a fundamental role in this form rejection, so far little is known concerning potential cellular component. Biopsies taken during DXR human CD55 transgenic hearts non-treated (n = 2), alpha-Gal immunoadsorbed or immunosuppressed 9) were studied. The element was explored determining not only its phenotype classical...
Abstract Lessons Learned Androgen receptor as assessed by immunohistochemistry is expressed in a high proportion of patients with hepatocellular carcinoma (HCC). Enzalutamide at 160 mg orally daily safe and tolerable advanced HCC but has no single-agent antitumor activity. Enzalutamide, CYP3A4 inducer, standard dose reduces the exposure sorafenib, substrate. sorafenib HCC, addition enzalutamide to did not enhance activity sorafenib. Background (AR) interference deleterious (HCC) preclinical...
As cancer care is transitioning to personalized therapies with necessary complementary or companion biomarkers there significant interest in determining what extent non-invasive liquid biopsies reflect the gold standard solid biopsy. We have established an approach for measuring patient-specific circulating and cell concordance by introducing tumor touch preparations High-Definition Single Cell Analysis workflow high-resolution cytomorphometric characterization of metastatic colorectal...
Multiple myeloma is an incurable malignancy that initiates from a bone marrow resident clonal plasma cell and acquires successive mutational changes genomic alterations, eventually resulting in tumor burden accumulation end-organ damage. It has been recently recognized secondary events result extensive sub-clonal heterogeneity both localized areas circulating peripheral blood cells. Rare subclones, including initiating cells, could be the drivers of disease progression recurrence....
B-cell maturation antigen (BCMA), a key regulator of proliferation and survival, is highly expressed in almost all cases plasma cell neoplasms B-lymphoproliferative malignancies. BCMA robust biomarker cells therapeutic target with substantial clinical significance. However, the expression circulating tumor patients hematological malignancies has not been validated for detection B cells. The application as single-cell profiling patients’ blood could enable early disease therapy response...
Abstract Non-small cell lung cancer (NSCLC) is the most frequent type of cancer, accounting for about 80 - 85% all diagnoses. Unfortunately, 75% NSCLC diagnoses are made when has already spread and prognosis poor. Therefore, biomarker investigation that might allow early detection critical to improving patient outcomes. The purpose this study demonstrate feasibility liquid biopsy detect identify circulating rare events, such as tumor cells (CTCs), associated with NSCLC. Here we apply our...