A5084720476- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Chemokine receptors and signaling
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- HER2/EGFR in Cancer Research
- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- interferon and immune responses
- Immune Response and Inflammation
- Endometriosis Research and Treatment
- Exercise and Physiological Responses
- RNA and protein synthesis mechanisms
- Reproductive System and Pregnancy
- Advanced Chemical Sensor Technologies
- Lung Cancer Treatments and Mutations
- Angiogenesis and VEGF in Cancer
- Monoclonal and Polyclonal Antibodies Research
- Cellular transport and secretion
- NF-κB Signaling Pathways
- Biochemical and Structural Characterization
- Dermatologic Treatments and Research
- Endometrial and Cervical Cancer Treatments
- Hepatitis C virus research
University of California, Irvine
2011-2021
Heinrich Heine University Düsseldorf
2015-2016
University of California, San Diego
2016
Best Biotech
2011
Neurocrine Biosciences (United States)
2005-2008
Eos Neuroscience (United States)
2001
Stanford University
1997
Brown University
1997
Columbia University
1991-1993
Endometriosis affects 10-20% of women reproductive age and is associated with pelvic pain infertility, its pathogenesis not well understood. We used genomewide transcriptional profiling to characterize endometriosis found that it exhibits a gene expression signature consistent an underlying autoimmune mechanism. lesions are characterized by the presence abundant plasma cells, many which produce IgM, macrophages BLyS/BAFF/TNFSF13B, member TNF superfamily implicated in other diseases. B...
Abstract Chemokines are chemotactic cytokines that direct the traffic of leukocytes and other cells in body. bind to G protein–coupled receptors expressed on target initiate signaling cascades induce chemotaxis. Although cognate most chemokines have been identified, receptor for mucosal chemokine CXCL17 is undefined. In this article, we show GPR35 CXCL17. tissues, CXCL17-responsive monocytes, THP-1 monocytoid cell line. Transfection into Ba/F3 rendered them responsive CXCL17, as measured by...
Abstract We have described a novel cytokine encoded by gene called Meteorin-like (Metrnl). Metrnl is small (∼28 kDa) secreted protein expressed activated macrophages and barrier tissues (mucosa skin). production bone marrow induced several cytokines including TNF-α, IL-17α, IL-12, IL-4 inhibited IFN-γ TGF-β. expression in also LPS, its levels circulation are associated with inflammatory responses vivo. Furthermore, regulates the of chemokines macrophages. produced Metrnl−/− mouse, which...
The Affymetrix GeneChip platform was used to build a gene expression database of the normal human body. Postmortem tissues represent valuable source biological materials for this type study, but their use entails some delays before harvesting such tissues. We first evaluated RNA quality obtained from 3-5 h postmortem and found variations that were both tissue donor-dependent. RNAs extracted brain regions higher than those gut, while cause death significant factor in donor-dependent...
The mucosal immune network is a crucial barrier preventing pathogens from entering the body. of cells that mediates defensive mechanisms in mucosa likely shaped by chemokines, which attract wide range to specific sites Chemokines have been divided into homeostatic or inflammatory depending upon their expression patterns. Additionally, several chemokines mediate direct killing invading pathogens, as exemplified CCL28, mucosa-associated chemokine exhibits antimicrobial activity against...
Taste bud cells transmit information regarding the contents of food from taste receptors embedded in apical microvilli to gustatory nerve fibers innervating basolateral membranes. In particular, depolarize, activate voltage-gated sodium channels, and fire action potentials response tastants. Initial cell depolarization is attributable influx through TRPM5 sweet, bitter, umami an undetermined cation ion channel sour expressing PKD2L1, a candidate receptor. The molecular identity channels that...
Efforts to unravel the mechanisms underlying taste sensation (gustation) have largely focused on rodents. Here we present first comprehensive characterization of gene expression in primate buds. Our findings reveal unique new insights into biology We generated a bud database using laser capture microdissection (LCM) procured fungiform (FG) and circumvallate (CV) buds from primates. also used LCM collect top bottom portions CV Affymetrix genome wide arrays were analyze all samples. Known...
Chemokines are a superfamily of chemotactic cytokines that direct the movement cells throughout body under homeostatic and inflammatory conditions. The mucosal chemokine CXCL17 was last ligand this to be characterized. Several recent studies have provided greater insight into basic biology implicated in several human diseases. We sought better characterize CXCL17's activity vivo. To end, we analyzed its chemoattractant properties vivo characterized Cxcl17 (-/-) mouse. This mouse has...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTGain-of-Function Mutagenesis of a Modular Polyketide SynthaseRobert McDaniel, Camilla M. Kao, Hong Fu, Peter Hevezi, Claes Gustafsson, Mary Betlach, Gary Ashley, David E. Cane, and Chaitan KhoslaView Author Information KOSAN Biosciences, Inc., 1450 Rollins Road Burlingame, California 94010 Departments Chemical Engineering, Chemistry, Biochemistry, Stanford University Stanford, 94305-5025 Department Box H, Brown Providence Rhode Island 02912...
Using a comprehensive microarray database of human gene expression, we identified that in mammals, secreted protein known as isthmin 1 (ISM1) is expressed skin, mucosal tissues, and selected lymphocyte populations. ISM1 was originally Xenopus brain during development, it encodes predicted ∼50-kDa containing signal peptide, thrombospondin domain, an adhesion-associated domain. We confirmed the pattern expression both mouse tissues. by DX5(+) lung lymphocytes include NK NKT-like cells, also...
Abstract Background The activation of the EGFR/Ras-signalling pathway in tumour cells induces a distinct chemokine repertoire, which turn modulates microenvironment. Methods effects EGFR/Ras on expression and translation CCL20 were analysed large set epithelial cancer cell lines tissues by RT-qPCR ELISA vitro. production was verified immunohistochemistry different correlated with clinical data. endothelial migration tumour-associated vascularisation comprehensively chemotaxis assays vitro...
Through bioinformatics analyses of a human gene expression database representing 105 different tissues and cell types, we identified 687 skin-associated genes that are selectively highly expressed in skin. Over 50 these represent uncharacterized not previously associated with skin include subset encode novel secreted plasma membrane proteins. The high levels for eight therapeutic target were confirmed by semi-quantitative real time PCR, western blot immunohistochemical normal skin-derived...
Abstract We describe a novel B cell–associated cytokine, encoded by an uncharacterized gene (C17orf99; chromosome 17 open reading frame 99), that is expressed in bone marrow and fetal liver whose expression also induced peripheral cells upon activation. C17orf99 only present mammalian genomes, it encodes small (∼27-kDa) secreted protein unrelated to other cytokine families, suggesting function immune responses. Accordingly, the mammary gland onset of lactation, C17orf99−/− mouse exhibits...
Adenomyosis is a common gynaecological disorder characterized by the abnormal growth of endometrium into myometrium and myometrial hypertrophy/hyperplasia. Uterine fibroids are benign neoplasms myometrium, they represent diagnostic pitfall for adenomyosis. In this study, we have used genome-wide Affymetrix U133 Plus 2.0 microarray platform to compare gene expression patterns adenomyosis, uterine fibroids, normal myometrium. Unsupervised principal component analysis (PCA) revealed that these...
Background Using fungiform (FG) and circumvallate (CV) taste buds isolated by laser capture microdissection analyzed using gene arrays, we previously constructed a comprehensive database of expression in primates, which revealed over 2,300 bud-associated genes. Bioinformatics analyses identified hundreds genes predicted to encode multi-transmembrane domain proteins with no previous association function. A first step elucidating the roles these products play gustation is identify specific...
Abstract Background and Objectives Neither the pathogenesis of port wine stain (PWS) birthmarks nor tissue effects pulsed dye laser (PDL) treatment these lesions is fully understood. There are few published reports utilizing gene expression analysis in human PWS skin. We aim to compare before after PDL, using DNA microarrays that represent most, if not all, genes obtain comprehensive molecular profiles PDL‐associated effects. Materials Methods Five subjects had PDL their PWS. One week later,...