A5011261157- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Pluripotent Stem Cells Research
- Alzheimer's disease research and treatments
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- CAR-T cell therapy research
- Tryptophan and brain disorders
- CRISPR and Genetic Engineering
- Toxoplasma gondii Research Studies
- Neurological Disease Mechanisms and Treatments
- Inflammasome and immune disorders
- Immunotherapy and Immune Responses
- Heme Oxygenase-1 and Carbon Monoxide
- Mesenchymal stem cell research
University of California, Irvine
2013-2023
Joslin Diabetes Center
2020
Harvard University
2020
The innate immune system is strongly implicated in the pathogenesis of Alzheimer's disease (AD). In contrast, role adaptive immunity AD remains largely unknown. However, numerous clinical trials are testing vaccination strategies for AD, suggesting that T and B cells play a pivotal this disease. To test hypothesis influences pathogenesis, we generated an immune-deficient mouse model lacks T, B, natural killer (NK) cells. resulting "Rag-5xfAD" mice exhibit greater than twofold increase...
ABSTRACT Interleukin-1β (IL-1β) functions as a key regulator of inflammation and innate immunity. The protozoan parasite Toxoplasma gondii actively infects human blood monocytes induces the production IL-1β; however, host factors that mediate IL-1β during T. infection are poorly understood. We report transcript, processing/cleavage, release from infected primary THP-1 cells. Treating with caspase-1 inhibitor Ac-YVAD-CMK reduced release, suggesting role for inflammasome in -induced...
T cells express clonotypic cell receptors (TCRs) that recognize peptide antigens in the context of class I or II MHC molecules (pMHCI/II). These receptor modules associate with three signaling (CD3γε, δε, and ζζ) work concert a coreceptor module (either CD8 CD4) to drive activation response pMHCI/II. Here, we describe first-generation biomimetic five-module chimeric antigen (5MCAR). We show 1) built ectodomains pMHCII assemble CD3 into complexes redirect cytotoxic lymphocyte (CTL)...
Abstract We describe a novel B cell–associated cytokine, encoded by an uncharacterized gene (C17orf99; chromosome 17 open reading frame 99), that is expressed in bone marrow and fetal liver whose expression also induced peripheral cells upon activation. C17orf99 only present mammalian genomes, it encodes small (∼27-kDa) secreted protein unrelated to other cytokine families, suggesting function immune responses. Accordingly, the mammary gland onset of lactation, C17orf99−/− mouse exhibits...
Abstract Hematopoietic cell transplantation (HCT) treats many blood conditions but remains underused due to complications such as graft‐versus‐host disease (GvHD). In GvHD, donor immune cells attack the patient, requiring powerful immunosuppressive drugs like glucocorticoids (GCs) prevent death. this study, we tested hypothesis that conditioning with glucocorticoid fluticasone propionate (FLU) prior could increase hematopoietic stem (HSC) engraftment and reduce GvHD. Murine HSCs treated FLU...
Abstract Hematopoietic stem cells (HSCs) are the self-renewing multipotent progenitors to all blood cell types. Identification and isolation of HSCs for study has depended on expression combinations surface markers that reliably distinguish them from other However, increasing number required isolate made it tedious, expensive, difficult newcomers, suggesting need a simpler panel HSC markers. We previously showed phenotypic could be separated based CD11a only negative fraction contained true...
The innate immune system is strongly implicated in Alzheimer's disease (AD) pathogenesis. In contrast, very few studies have examined the potential role of adaptive response this disorder. extensive testing and clinical assessment beta-amyloid (Aβ) vaccination strategies has only heightened need to better understand relationships interplay between AD neuropathology. We recently demonstrated through generation an immune-deficient mouse model which lacks T-, B-, NK-cells, that loss these cells...
Abstract Hematopoietic stem cells (HSCs) are the self-renewing multipotent progenitors to all blood cell types. Identification and isolation of HSCs for study has depended on expression combinations surface markers that reliably distinguish it from other However, increasing number required isolate made tedious, expensive, difficult newcomers, suggesting need a simpler panel HSC markers. We previously showed phenotypic could be separated based CD11a, only CD11a negative fraction contained...
Abstract T cells express clonotypic cell receptors (TCRs) that recognize peptide antigens in the context of class I or II MHC molecules (pMHCI/II). These receptor modules associate with three signaling (CD3γε, δε, and ζζ), work concert a coreceptor module (either CD8 CD4), to drive activation response pMHCI/II. Here we describe first generation biomimetic 5-module chimeric antigen ( 5M CAR). We show that: (i) built ectodomains pMHCII assemble CD3 into complexes redirect cytotoxic lymphocyte...
Hematopoietic stem cells (HSCs) are defined by their self-renewal, multipotency, and bone marrow (BM) engraftment abilities. How HSCs emerge during embryonic development remains unclear, but thought to arise from hemogenic endothelium through an intermediate precursor called “pre-HSCs.” Pre-HSCs have self-renewal multipotent activity, lack BM engraftability. They can be identified functionally transplantation into neonatal recipients, or in vitro co-culture with cytokines stroma followed...