A5011554115- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Erythrocyte Function and Pathophysiology
- Microtubule and mitosis dynamics
- Galectins and Cancer Biology
- Advanced Fluorescence Microscopy Techniques
- 3D Printing in Biomedical Research
- Lipid Membrane Structure and Behavior
- Cancer Cells and Metastasis
- PI3K/AKT/mTOR signaling in cancer
- Cell Adhesion Molecules Research
- Osteoarthritis Treatment and Mechanisms
- Hemoglobin structure and function
- Veterinary Equine Medical Research
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- Nuclear Structure and Function
- Viral Infectious Diseases and Gene Expression in Insects
- ATP Synthase and ATPases Research
- Force Microscopy Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Immune Cell Function and Interaction
- Proteoglycans and glycosaminoglycans research
- Genetics, Aging, and Longevity in Model Organisms
Cornell University
2014-2025
Smith Institute
2024
Saint Meinrad Seminary and School of Theology
2024
John Wiley & Sons (United States)
2019
Hudson Institute
2019
University of California, San Francisco
2009-2014
California Institute for Regenerative Medicine
2013-2014
University of Pennsylvania
2004-2009
How adherent and contractile systems coordinate to promote cell shape changes is unclear. Here, we define a counterbalanced adhesion/contraction model for control. Live-cell microscopy data showed crucial role meshwork at the top of cell, which composed actin arcs myosin IIA filaments. The organized like muscle sarcomeres, with repeating II filaments separated by bundling protein α-actinin, mechanically coupled noncontractile dorsal fibers that run from bottom in cell. When contracts, it...
Extracellular matrix (ECM) stiffness induces focal adhesion assembly to drive malignant transformation and tumor metastasis. Nevertheless, how force alters adhesions promote progression remains unclear. Here, we explored the role of protein vinculin, a force-activated mechanotransducer, in mammary epithelial tissue invasion. We found that ECM stabilizes vinculin-talin-actin scaffolding complex facilitates PI3K-mediated phosphatidylinositol (3,4,5)-triphosphate phosphorylation. Using defined...
Integrins have emerged as key sensory molecules that translate chemical and physical cues from the extracellular matrix (ECM) into biochemical signals regulate cell behavior. function by clustering adhesion plaques, but molecular mechanisms drive integrin in response to interaction with ECM remain unclear. To explore how deformations cell-ECM interface influence clustering, we developed a spatial-temporal simulation integrates micro-mechanics of cell, glycocalyx, simple model activation...
To investigate the transition from non-cancerous to metastatic a physical sciences perspective, Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of non-tumorigenic MCF-10A MDA-MB-231 breast epithelial cell lines, commonly used as models cancer metastasis. Experiments were in 20 laboratories 12 PS-OCs. Each laboratory was supplied with identical aliquots common reagents culture protocols. Analyses these measurements revealed dramatic...
As interest in the role of extracellular vesicles cell-to-cell communication has increased, so use microscopy and analytical techniques to assess their formation, release, morphology. In this study, we evaluate scanning electron (SEM) cryo-SEM for characterizing formation shedding from human breast cell lines, parental hyaluronan synthase 3-(HAS3)-overexpressing MCF10A cells, grown directly on transmission (TEM) grids. While cells imaged with conventional exhibit distinct morphologies due...
The glycocalyx is a coating of protein and sugar on the surface all living cells. Dramatic perturbations to composition structure are frequently observed in aggressive cancers. However, tools experimentally mimic model cancer-specific remain limited. Here, we develop genetically encoded toolkit engineer chemical physical cellular glycocalyx. By manipulating structure, able switch adhesive state cells from strongly adherent fully detached. Surprisingly, find that thick dense with high...
Mesenchymal stromal cells (MSCs) can be used intra-articularly to quell inflammation and promote cartilage healing; however, mechanisms by which MSCs mitigate joint disease remain poorly understood. Galectins, a family of β-galactoside binding proteins, regulate inflammation, adhesion cell migration in diverse types. Galectin-1 galectin-3 are proposed important intra-articular modulators both osteoarthritis rheumatoid arthritis. Here, we asked whether equine bone marrow-derived (BMSCs)...
Skeletal metastasis is common in patients with advanced breast cancer and often caused by immune evasion of disseminated tumor cells (DTCs). In the skeleton, not only disseminate to bone marrow but also osteogenic niches which they interact newly mineralizing extracellular matrix (ECM). However, it remains unclear how mineralization collagen type I, primary component ECM, regulates tumor-immune cell interactions. Here, a combination synthetic models controlled mineral content, nanoscale...
Abstract Osteochondral lesions (OCL) are common among young patients and often require surgical interventions since cartilage has a poor capacity for self-repair. Bone marrow stimulation (BMS) been used clinically decades to treat OCLs, however persisting challenge with BMS other repair strategies is the inferior quality of resulting fibrocartilaginous tissue. Lubrication-based therapies have potential improve tissue as joint lubrication linked local strains subsequent cellular responses...
Cells feel the tension of implanted and injected silicone materials.
Lameness is a debilitating condition in equine athletes that leads to more performance limitation and loss of use than any other medical condition. There are limited number non-terminal experimental models can be used study early inflammatory synovial fluid biophysical changes occur the joint. Here, we compare well-established carpal IL-1β-induced synovitis model tarsal intra-articular lavage model, focusing on serial cytokines/chemokines lubricating molecules lubricin/proteoglycan 4...
Lamins A/C are nuclear intermediate filament proteins that involved in diverse cellular mechanical and biochemical functions. Here, we report recognition of by a commonly used antibody (JOL-2) binds the Lamin Ig-fold other antibodies targeting similar epitopes is highly dependent on cell density, even though A/Clevels do not change. We propose effect caused partial unfolding or masking C'E and/or EF loops response to spreading. Surprisingly, JOL-2 labeling was insensitive disruption...