A5083524285- Lipid Membrane Structure and Behavior
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Spectroscopy and Quantum Chemical Studies
- Crystallography and molecular interactions
- Nanopore and Nanochannel Transport Studies
- Supramolecular Self-Assembly in Materials
- Protein Structure and Dynamics
- Surfactants and Colloidal Systems
- Force Microscopy Techniques and Applications
- Antimicrobial Peptides and Activities
- Polydiacetylene-based materials and applications
- Electrostatics and Colloid Interactions
- Erythrocyte Function and Pathophysiology
- Sphingolipid Metabolism and Signaling
- Autophagy in Disease and Therapy
- Glycosylation and Glycoproteins Research
- Antimicrobial agents and applications
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
- Protein Hydrolysis and Bioactive Peptides
- Microfluidic and Bio-sensing Technologies
- Lipid metabolism and biosynthesis
- Photosynthetic Processes and Mechanisms
- Legionella and Acanthamoeba research
Universidade de São Paulo
2012-2025
Cornell University
2015-2024
University of Tennessee at Knoxville
2020-2024
It is well known that the lipid distribution in bilayer leaflets of mammalian plasma membranes (PMs) not symmetric. Despite this, model membrane studies have largely relied on chemically symmetric for study lipid–lipid and lipid–protein interactions. This primarily due to difficulty preparing stable, asymmetric are amenable biophysical studies. However, last 20 years, efforts been made producing more biologically faithful membranes. Here, we review several recently developed experimental...
We have determined the partial leaflet–leaflet phase diagram of an asymmetric lipid bilayer at ambient temperature using giant unilamellar vesicles (aGUVs). Symmetric GUVs with varying amounts 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) were hemifused to a supported (SLB) composed DOPC, resulting in exchange between their outer leaflets. The SLB contained red green fluorophore, respectively, thus enabling use confocal fluorescence imaging...
Highly charged vesicles of the saturated anionic lipid dimyristoyl phosphatidylglycerol (DMPG) in low ionic strength medium exhibit a very peculiar thermo-structural behavior. Along wide gel–fluid transition region, DMPG dispersions display several anomalous characteristics, like turbidity, high electrical conductivity and viscosity. Here, static dynamic light scattering (SLS DLS) were used to characterize at different temperatures. Similar experiments performed with largely studied...
Considering the known different mode of action antimicrobial peptides in zwitterionic and anionic cell membranes, present work compares peptide K0-W6-Hya1 (KIFGAIWPLALGALKNLIK-NH2) with negatively charged model namely, liposomes composed phosphatidylcholine (PC) phosphatidylglycerol (PG) a mixture two. Differential scanning calorimetry (DSC), steady state fluorescence Trp residue, dynamic light scattering (DLS), measurement leakage an entrapped fluorescent dye (carboxyfluorescein, CF) were...
The data provided with this paper are confocal fluorescence images of symmetric giant unilamellar vesicles (GUVs) and asymmetric (aGUVs). In work, aGUVs were prepared using the hemifusion method labelled two different fluorescent dyes, named TFPC DiD. Both dyes show strong preference for liquid-disordered (Ld) phase instead liquid-ordered (Lo) phase. partition these favoring Ld leads to bright dark Lo domains in GUVs observed by microscopy. vesicles, inner outer leaflets aligned. aGUVs,...
Abstract Legionella pneumophila is a facultative intracellular pathogen that causes Legionnaires’ disease or Pontiac fever in humans upon accidental inhalation of -contaminated aerosols. During infection, L. secretes more than 300 effectors into the host for biogenesis replication-permissive niche, known as containing vacuole (LCV). Among these, large number harbor protein domains recognize specific phosphoinositide (PI) lipids and mediate anchoring these to surface LCV other membrane-bound...
This mini review intends to highlight the importance of bilayer asymmetry. Biological membranes are complex structures that a physical barrier separating external environment from cellular content. comprises an extensive lipid repertory, suggesting different might subserve role in membrane. Interestingly, this vast repertory lipids is asymmetrically distributed between leaflets form bilayer. Here, we discuss properties plasma membrane under view experimental model membranes, consisting...