A5086986935- Antimicrobial Resistance in Staphylococcus
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Bacteriophages and microbial interactions
- Microbial Natural Products and Biosynthesis
- Bacillus and Francisella bacterial research
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Microbial Metabolism and Applications
- Invertebrate Immune Response Mechanisms
- COVID-19 Clinical Research Studies
- Antibiotic Resistance in Bacteria
- COVID-19 epidemiological studies
- Insect Utilization and Effects
- COVID-19 Pandemic Impacts
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Vaccine Coverage and Hesitancy
- Fluorine in Organic Chemistry
- Clostridium difficile and Clostridium perfringens research
- Quinazolinone synthesis and applications
- Bioactive Natural Diterpenoids Research
- Oral microbiology and periodontitis research
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
Hokkaido University
2020-2024
University of Tennessee at Knoxville
2024
Teikyo University
2016-2021
Otsuka (Japan)
2016-2021
Tribhuvan University
2016
The University of Tokyo
2011-2014
Increased antimicrobial resistance (AMR) among bacteria underscores the need to strengthen AMR surveillance and promote data-based prescribing. To evaluate trends associations between usage (AMU) AMR, we explored a dataset of 34,672 bacterial isolates collected 2015 2020 from clinical samples at University Teaching Hospital (UTH) in Lusaka, Zambia. The most frequently isolated species were Escherichia coli (4,986/34,672; 14.4%), Staphylococcus aureus (3,941/34,672; 11.4%), Klebsiella...
Abstract Lysocin E, a 37-membered natural depsipeptide, induces rapid bacteriolysis in methicillin-resistant Staphylococcus aureus via unique menaquinone-dependent mechanism, presenting promising therapeutic lead. Despite the great medical importance, exploring potential utility of its derivatives as new platform structures for antibiotic development has remained significant challenge. Here, we report high-throughput strategy that enabled preparation thousands analogues lysocin E and...
The epidemiology of extended-spectrum β-lactamases (ESBLs) has undergone dramatic changes, with CTX-M-type enzymes prevailing over other types. blaCTX-M genes, encoding ESBLs, are usually found on plasmids, but chromosomal location is becoming common. Given that blaCTX-M-harboring strains often exhibit multidrug resistance (MDR), it important to investigate the association between chromosomally integrated and presence additional antimicrobial (AMR) identify relevant genetic elements.A total...
Enterococcus faecalis is a common human gut commensal bacterium. While some E. strains are probiotic, others known to cause opportunistic infections, and clear distinction between these difficult using traditional taxonomic approaches. In this study, we completed the genome sequencing of EF-2001, probiotic strain, our in-house hybrid assembly approach. Comparative analysis showed that EF-2001 was devoid cytolysins, major factors associated with pathogenesis, phylogenetically distant from...
Abstract Polyoxins J ( 1 a ) and L b are important nucleoside antibiotics. The complex densely functionalized dipeptide structures of contain thymine uracil nucleobases, respectively. Herein we report the unified total synthesis , their artificial analogues c d with trifluorothymine fluorouracil structures. Decarbonylative radical coupling between α‐alkoxyacyl tellurides chiral glyoxylic oxime ether led to chemo‐ stereoselective construction ribonucleoside α‐amino acid – without damaging...
Abstract Gramicidin A ( 1 ) is a peptide antibiotic that disrupts the transmembrane ion concentration gradient by forming an channel in lipid bilayer. Although long used clinically, it limited to topical application because of its strong hemolytic activity and mammalian cytotoxicity, likely arising from common transport mechanism. Here we report integrated high-throughput strategy for discovering analogues with altered biological profiles. The 4096 analogue structures are designed maintain...
We previously reported that disruption of the yjbI gene reduced virulence Staphylococcus aureus. In this study, we found in both silkworms and mice was restored by introducing yjbH but not to genes-disrupted mutants, suggesting yjbH, downstream a two-gene operon-yjbIH, is responsible for phenomenon. further observed decrease various surface-associated proteins changes cell envelope glycostructures mutants. RNA-seq analysis revealed genes resulted differential expression broad range genes,...
WAP-8294A2 (lotilibcin, 1) is a potent antibiotic with superior in vivo efficacy to vancomycin against methicillin-resistant Staphylococcus aureus (MRSA). Despite the great medical importance, its molecular mode of action remains unknown. Here we report total synthesis complex macrocyclic peptide 1 comprised 12 amino acids β-hydroxy fatty-acid chain, and deoxy analogue 2. A full solid-phase 2 enabled their rapid assembly first detailed investigation functions. Compounds were equipotent...
Weissella genus from Leuconostocaceae family forms a group of Gram-positive lactic acid bacteria (LAB) that mostly reside in fermented foods and some have been isolated the environment vertebrates including humans. Currently there are 23 recognized species, 16 complete 37 draft genome assemblies for this genus. hellenica has found various sources is characterized by their probiotic bacteriocinogenic properties. Despite its widespread importance, little attention paid to genomic...
Abstract Poor pharmacokinetic parameters are a major reason for the lack of therapeutic activity some drug candidates. Determining candidates at an early stage development requires inexpensive animal model with few associated ethical issues. In this study, we used silkworm infection to perform structure-activity relationship studies antimicrobial agent, GPI0039, novel nitrofuran dichloro-benzyl ester, and successfully identified compound 5 , nitrothiophene as potent agent superior in model....
Candida albicans TIMM1768 is a highly virulent strain utilized as model organism for the study of gastrointestinal and oral candidiasis. Despite being strain, identification its genetic determinants pathogenesis hindered by unavailability genome sequence. In this study, we determined sequence C. using reads obtained from portable MinION benchtop Ion PGM sequencers. Genome annotation comparative analysis with published genomes revealed that was close to CHN1, identified significant number...
The regulatory network of virulence factors produced by the opportunistic pathogen Staphylococcus aureus is unclear and functions many uncharacterized genes in its genome remain to be elucidated. In this study, we screened 380 whose function was unassigned, utilizing gene-disrupted transposon mutants community-acquired methicillin-resistant S. USA300 for pathogenicity silkworms. We identified 10 strains with reduced silkworm killing ability. Among them, 8 displayed a mouse model as evidenced...
Multidrug-resistant (MDR) Escherichia coli in food animals such as chickens is an emerging public health concern Zambia. Additionally, the country's high demand for poultry products necessitates further investigation into link between and human MDR E. coli. Twenty cefotaxime-resistant isolates collected from Lusaka, Zambia, were screened multidrug resistance sequenced on MiSeq MinION platforms. Genomes assembled de novo compared with 36 previously reported inpatients at University Teaching...
Abstract Lysocin E is a lipopeptide with antibiotic activity against methicillin-resistant Staphylococcus aureus . For unclear reasons, the antibacterial of lysocin in mouse systemic infection model higher than expected from vitro results, and enhanced by addition bovine serum. Here, we confirm that serum various species, including humans, increases antimicrobial activity, identify apolipoprotein A-I (ApoA-I) as an enhancing factor. ApoA-I when added vitro, displays reduced gene knockout...
The total synthesis of plusbacin A3 (1) has been accomplished using a solvent-dependent diastereodivergent Joullié–Ugi three-component reaction (JU-3CR) as key step. Two trans-3-hydroxy-l-proline residues were constructed by combining the JU-3CR with convertible isocyanide strategy. Subsequent peptide coupling and macrolactamization afforded A3. Investigating antibacterial activity 1 compared that its dideoxy analogue revealed threo-β-hydroxyaspartic acid are essential for activity. Notably,...
Synthetic compounds are a vital source of antimicrobial agents. To uncover therapeutically effective agents from chemical library, we screened over 100,000 synthetic for in vitro activity against methicillin-resistant Staphylococcus aureus and evaluated the vivo therapeutic effectiveness hits S. aureus-infected silkworms. Three exhibited effects silkworm infection model. One these, GPI0363, novel spiro-heterocyclic compound, was bacteriostatic inhibited RNA synthesis cells. GPI0363-resistant...