A5034613776- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Vector-borne infectious diseases
- Animal Disease Management and Epidemiology
- Trypanosoma species research and implications
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Insect symbiosis and bacterial influences
- Virology and Viral Diseases
- Probiotics and Fermented Foods
- Antibiotic Resistance in Bacteria
- Biosensors and Analytical Detection
- Yersinia bacterium, plague, ectoparasites research
- T-cell and Retrovirus Studies
- Aquatic Ecosystems and Biodiversity
- COVID-19 epidemiological studies
- Viral Infections and Outbreaks Research
- Malaria Research and Control
- Respiratory viral infections research
- Fish Biology and Ecology Studies
- Rabies epidemiology and control
- Microbial infections and disease research
University of Zambia
2016-2025
Hokkaido University
2020-2024
Centers for Disease Control and Prevention
2020
Lusaka Apex Medical University
2020
Ministry of Agriculture and Livestock
2020
University of Edinburgh
2020
University of Florida
2020
Ministry of Livestock and Fisheries
2018
During 2013–2015, several and severe outbreaks of African swine fever (ASF) affected domestic pigs in six provinces Zambia. Genetic characterization ASF viruses (ASFVs) using standardized genotyping procedures revealed that genotypes I, II XIV were associated with these outbreaks. Molecular epidemiological data suggest genotype ASFV (Georgia 2007/1-like) detected Northern Province Zambia may have been introduced from neighbouring Tanzania. Also, a virus Eastern showed p54 phylogenetic...
Relapsing fever is an infectious disease previously neglected in Africa, which imposes a large public health burden the country. We aimed to investigate and report on case of relapsing borreliosis Zambia. A unknown Borrelia species was isolated from blood febrile patient. Investigations presumptive vector ticks natural hosts for were conducted by culture isolation and/or DNA detection Borrelia-specific polymerase chain reaction. Using isolates patient bat specimens, genetic characterization...
Because of the low sensitivity conventional rapid diagnostic tests (RDTs) for malaria infections, actual prevalence diseases, especially those caused by non-Plasmodium falciparum (non-Pf) species, in asymptomatic populations remain less defined countries lacking well-equipped facilities accurate diagnoses. Our direct blood dry LAMP system (CZC-LAMP) was applied to diagnosis as simple, and highly sensitive method an alternative RDTs endemic areas where laboratory resources are limited. primer...
Dogs have been implicated to serve as links for parasite exchange between livestock and humans remain an important source of emerging re-emerging diseases including trypanosome infections. Yet, canine African trypanosomosis (CAT), particularly in indigenous dogs (mongrel breed) remains under- reported literature. This study evaluated the performance loop-mediated isothermal amplification (LAMP) detecting trypanosomes blood from tsetse-infested Mambwe district eastern Zambia.A cross sectional...
Loop-mediated isothermal amplification (LAMP) is a novel strategy which amplifies DNA with high sensitivity and rapidity under conditions. In the present study, performance of repetitive insertion mobile element (RIME)-LAMP human serum resistance-associated gene (SRA)-LAMP assays were evaluated using clinical specimens obtained from four male patients Luangwa Zambezi valleys in Zambia Zimbabwe, respectively. The cases reported this preliminary communication all first diagnosed by microscopy,...
Bats are suspected to play important roles in the ecology of filoviruses, including ebolaviruses and marburgviruses. A cave-dwelling fruit bat, Rousettus aegyptiacus, has been shown be a reservoir Using an enzyme-linked immunosorbent assay with viral glycoprotein antigen, we detected immunoglobulin G antibodies specific multiple filoviruses 158 290 serum samples R aegyptiacus bats captured Zambia during years 2014–2017. In particular, 43.8% were seropositive marburgvirus, supporting notion...
Poultry production is essential to the economy and livelihood of many rural Zambian households. However, industry threatened by infectious diseases, particularly Newcastle disease virus (NDV) infection. Therefore, this study employed next-generation sequencing characterise six NDV isolates from poultry in Zambia’s live bird markets (LBMs) wild waterfowl. Four were detected 410 faecal samples collected chickens LBMs Lusaka two 2851 birds Lochinvar National Park. Phylogenetic analysis revealed...
Enteric infections due to viral pathogens are a major public health concern. Detecting the risk areas requires strong surveillance system for pathogenic viruses in sources such as wastewater. Towards building an environmental Zambia, we aimed identify group A rotavirus (RVA) and human adenovirus (HAdV) Convenient sampling was conducted at four study sites every Tuesday five consecutive weeks. The research team focused on three different methods of concentration determine suitability terms...
African swine fever (ASF) is a contagious haemorrhagic disease associated with causing heavy economic losses to the industry in many countries. In 2017, Zambia experienced ASF outbreaks Mbala District (Northern province) and for first time Isoka Chinsali districts (Muchinga province). Meanwhile, another outbreak was observed Chipata (Eastern Genetic analysis of part B646L gene, E183L CP204L gene central variable region B602L virus (ASFV) conducted. The results revealed that ASFV detected...
We detected West Nile virus (WNV) nucleic acid in crocodiles (Crocodylus niloticus) Zambia. Phylogenetically, the belonged to lineage 1a, which is predominant Northern Hemisphere. These data provide evidence that WNV circulating Africa and increases risk for animal human transmission.
A cross-sectional study was used to identify and assess prevalence phenotypic antimicrobial resistance (AMR) profiles of Escherichia coli other enterobacteria isolated from healthy wildlife livestock cohabiting at a 10,000 acres game ranch near Lusaka, Zambia. Purposive sampling select based on similarities in behavior, grazing habits close interactions with humans. Isolates (n = 66) fecal samples collected between April August 2018 84) were examined following modified protocols for bacteria...
Poultry products in Zambia form an integral part of the human diet many households, as they are cheap and easy to produce. The burden poultry diseases has, however, remained a major challenge. Growing consumer demand for has resulted non-prudent antimicrobial use on farms, intending prevent treat growth optimisation maximising profits. This cross-sectional study aimed identify different types bacteria causing chickens Lusaka detect extended-spectrum lactamase (ESBL)-encoding genes. We...
Crimean-Congo hemorrhagic fever virus (CCHFV) is endemic in Africa, Asia, and Eastern Europe where it circulates among animals ticks causing sporadic outbreaks humans. Although CCHF sub-Saharan epidemiological information lacking many countries, including Malawi. To assess the risk of Malawi, we conducted an study cattle reared by smallholder livestock farmers central A cross-sectional was April 2020 involving seven districts, four from Kasungu three Lilongwe Agriculture Development...
Background An estimated 75% or more of the human rabies cases in Africa occur rural settings, which underscores importance control these areas. Understanding dog demographics can help design strategies for and plan conduct canine mass vaccination campaigns effectively African countries. Methodology/Principal findings A cross-sectional survey was conducted to investigate domestic Kalambabakali, Mazabuka District Zambia. The population ownerless dogs total achievable coverage among using...
Tick-borne diseases (TBDs), including emerging and re-emerging zoonoses, are of public health importance worldwide; however, TBDs tend to be overlooked, especially in countries with fewer resources, such as Zambia Angola. Here, we investigated Rickettsia, Anaplasmataceae, Apicomplexan pathogens 59 96 adult ticks collected from dogs cattle, respectively, Shangombo, a town at the Zambia-Angola border. We detected Richkettsia africae Rickettsia aeschilimannii 15.6% Amblyomma variegatum 41.7%...
In recent years, the southern African region has experienced repeated incursions of highly pathogenic avian influenza viruses (HPAIVs), with wild migratory birds being implicated in spread. To understand profile (AIVs) circulating Zambia, we surveyed waterfowl for AIVs and phylogenetically characterised isolates detected 2015, 2020, 2021. A total 2,851 faecal samples were collected from Lochinvar National Park Southern Province Zambia. During study period, 85 (3.0%) low pathogenicity...
Nosocomial infections are a serious public health problem affecting both developed and developing countries. They caused mainly by multi-drug-resistant pathogens that limit treatment options, leading to high morbidity mortality, longer hospital stays increased costs of care. This study aimed evaluate nosocomial infections, risk factors causative at two large teaching hospitals in Zambia. A yearlong hospital-based cross-sectional was conducted from April 2020 2021 tertiary-level Hospitalised...
Enteric infections are a major public health concern. Detecting risk areas requires strong surveillance system for pathogenic viruses in possible sources such as wastewater. To build an environmental system, we aimed to identify group A rotavirus (RVA) and human adenovirus (HAdV) We also focused on three different methods of viral concentration determine suitability regular system. screened 20 wastewater samples HAdV RVA using quantitative polymerase chain reaction (qPCR) conventional...
Rotavirus B (RVB) causes diarrhea in humans and pigs. Although various RVB strains were identified animals globally, little is known about the epidemiology infection Africa. In this study, we attempted to examine prevalence of pig populations Zambia.
Tsetse flies are obligate hematophagous vectors of animal and human African trypanosomosis. They cyclically transmit pathogenic Trypanosoma species. The endosymbiont Sodalis glossinidius is suggested to play a role in facilitating the susceptibility tsetse trypanosome infections. Therefore, this study was aimed at determining prevalence S. trypanosomes circulating checking whether an association exists between infections from Kafue National Park Zambia. A total 326 were sampled Chunga Ngoma...
Rotavirus is a major cause of diarrhea globally in animals and young children under 5 years. Here, molecular detection genetic characterization porcine rotavirus smallholder commercial pig farms the Lusaka Province Zambia were con-ducted. Screening 148 stool samples by RT-PCR targeting VP6 gene revealed prevalence 22.9 % (34/148). Further testing VP6-positive with VP7-specific primers produced 12 positives, which then Sanger-sequenced. BLASTn VP7 positives showed sequence similarity to human...