A5035711553- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Phytase and its Applications
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Hemoglobin structure and function
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Sleep and related disorders
- Cellular transport and secretion
- Intracranial Aneurysms: Treatment and Complications
- Cerebrospinal fluid and hydrocephalus
- Ubiquitin and proteasome pathways
- Plant Stress Responses and Tolerance
- Health and Wellbeing Research
- Dementia and Cognitive Impairment Research
- Sepsis Diagnosis and Treatment
- Plant Gene Expression Analysis
- Epigenetics and DNA Methylation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Genomics and Diagnostics
- Agronomic Practices and Intercropping Systems
- Plant Reproductive Biology
- Leaf Properties and Growth Measurement
CSL (Switzerland)
2024
University of Geneva
2018-2024
Clinics Hospital of Ribeirão Preto
2022
University of Minho
2016-2022
Sainsbury Laboratory
2015-2021
University of East Anglia
2019-2021
Conservatory and Botanical Garden of the City of Geneva
2019-2021
Universidade de São Paulo
2021
Norwich Research Park
2015-2018
Plants recognize pathogen-associated molecular patterns (PAMPs) via cell surface-localized pattern recognition receptors (PRRs), leading to PRR-triggered immunity (PTI). The Arabidopsis cytoplasmic kinase BIK1 is a downstream substrate of several PRR complexes. How plant PTI negatively regulated not fully understood. Here, we identify the protein phosphatase PP2C38 as negative regulator activity and BIK1-mediated immunity. dynamically associates with BIK1, well PRRs FLS2 EFR, but co-receptor...
Respiratory symptoms are one of COVID-19 manifestations, and the metalloproteinases (MMPs) have essential roles in lung physiology. We sought to characterize plasmatic levels matrix metalloproteinase-2 9 (MMP-2 MMP-9) patients with severe investigate an association between plasma MMP-2 MMP-9 clinical outcomes mortality. from treated ICU (COVID-19 group) Control were measured zymography. The study groups matched for age, sex, hypertension, diabetes, BMI, obesity profile. lower higher a group...
The activation of phosphoinositide-specific phospholipase C (PI-PLC) is one the earliest responses triggered by recognition several microbe-associated molecular patterns (MAMPs) in plants. Arabidopsis (Arabidopsis thaliana) PI-PLC gene family composed nine members. Previous studies suggested a role for PLC2 MAMP-triggered immunity, as it rapidly phosphorylated vivo upon treatment with bacterial MAMP flg22. Here, we analyzed plant immunity using an artificial microRNA to silence expression...
Significance Protein kinases lacking Arg in the catalytic loop HxD motif (i.e., non-RD kinases) are associated with innate immune signaling across kingdoms. Phosphorylation activates plant receptor (RKs), but mechanistic details of activation limited. Using RK ELONGATION FACTOR TU RECEPTOR (EFR) as a model, we investigated role cytoplasmic domain and found that activity EFR is dispensable for antibacterial immunity. Nevertheless, ligand-induced EFR-mediated initiated by phosphorylation, not...
Secondary brain injury (SBI) occurs with a lag of several days post-bleeding in patients aneurysmal subarachnoid hemorrhage (aSAH) and is strong contributor to mortality long-term morbidity. aSAH-SBI coincides cell-free hemoglobin (Hb) release into the cerebrospinal fluid. This temporal association convincing pathophysiological concepts suggest that CSF-Hb could be targetable trigger SBI. However, sparse experimental evidence for Hb's neurotoxicity vivo defines significant research gap...
The establishment of nitrogen-fixing root nodules in legume-rhizobia symbiosis requires an intricate communication between the host plant and its symbiont. We are, however, limited our understanding signaling process. In particular, how membrane-localized receptors legumes activate signal transduction following perception rhizobial molecules has mostly remained elusive. To address this, we performed a coimmunoprecipitation-based proteomics screen to identify proteins associated with Nod...
Inositol pyrophosphates (PP-InsPs) are nutrient messengers whose cellular levels precisely regulated. Diphosphoinositol pentakisphosphate kinases (PPIP5Ks) generate the active signaling molecule 1,5-InsP 8 . PPIP5Ks harbor phosphatase domains that hydrolyze PP-InsPs. Plant and Fungi Atypical Dual Specificity Phosphatases (PFA-DSPs) NUDIX phosphatases (NUDTs) also involved in PP-InsP degradation. Here, we analyze relative contributions of three different families to plant catabolism. We...
Abstract The ubiquitin-like modifying peptide SMALL UBIQUITIN-LIKE MODIFIER (SUMO) has become a known modulator of the plant response to multiple environmental stimuli. A common feature many these external stresses is production reactive oxygen species (ROS). Taking into account that SUMO conjugates rapidly accumulate in an oxidative stimulus, it likely ROS and sumoylation converge at molecular regulatory levels. In this study, we explored SUMO–ROS relationship, using as model Arabidopsis...
Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One most direct approaches to quantify is isolation RNA. Most techniques used require RNA, usually from a large number samples. While published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or costly, previously protocol RNA Arabidopsis thaliana yields high amounts good...
Inositol pyrophosphates (PP-InsPs) are highly phosphorylated molecules that have emerged as central nutrient messengers in eukaryotic organisms. They can bind to structurally diverse target proteins regulate biological functions, such protein–protein interactions. PP-InsPs strongly negatively charged and interact with basic surface patches proteins, making their quantitative biochemical analysis challenging. Here, we present the synthesis of biotinylated myo-inositol hexakisphosphates...
Abstract Inositol pyrophosphates (PP-InsPs) are nutrient messengers whose cellular concentration must be tightly regulated. Diphosphoinositol pentakisphosphate kinases (PPIP5Ks) generate the active signaling molecule 1,5-InsP 8 . PPIP5Ks contain additional phosphatase domains involved in PP-InsP catabolism. Plant and Fungi Atypical Dual Specificity Phosphatases (PFA-DSPs) NUDIX phosphatases (NUDTs) also hydrolyze PP-InsPs. Here we dissect relative contributions of three different families to...
Abstract Receptor kinases (RKs) play fundamental roles in extracellular sensing to regulate development and stress responses across kingdoms. In plants, leucine-rich repeat receptor (LRR-RKs) function primarily as peptide receptors that myriad aspects of plant response external stimuli. Extensive phosphorylation LRR-RK cytoplasmic domains is among the earliest detectable following ligand perception, reciprocal transphosphorylation between a its co-receptor thought activate complex....
<title>Abstract</title> Background Secondary brain injury (SBI) occurs with a lag of several days post-bleeding in patients aneurysmal subarachnoid hemorrhage (aSAH) and is strong contributor to mortality long-term morbidity. aSAH-SBI coincides cell-free hemoglobin (Hb) release into the cerebrospinal fluid. This temporal association convincing pathophysiological concepts suggest that CSF-Hb could be targetable trigger SBI. However, sparse experimental evidence for Hb’s neurotoxicity vivo...
<title>Abstract</title> Background Secondary brain injury (SBI) occurs with a lag of several days post-bleeding in patients aneurysmal subarachnoid hemorrhage (aSAH) and is strong contributor to mortality long-term morbidity. aSAH-SBI coincides cell-free hemoglobin (Hb) release into the cerebrospinal fluid. This temporal association convincing pathophysiological concepts suggest that CSF-Hb could be targetable trigger SBI. However, sparse experimental evidence for Hb’s neurotoxicity vivo...