A5042243109- Microbial Metabolites in Food Biotechnology
- Plant Pathogenic Bacteria Studies
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Enzyme Production and Characterization
- Phytoplasmas and Hemiptera pathogens
- Plant and Fungal Interactions Research
- Food composition and properties
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Plant pathogens and resistance mechanisms
- Insect Pest Control Strategies
- Insect symbiosis and bacterial influences
- Polysaccharides and Plant Cell Walls
- Insect-Plant Interactions and Control
- Insect behavior and control techniques
- Plant Stress Responses and Tolerance
- Transgenic Plants and Applications
- Insect Resistance and Genetics
- Magnetic and Electromagnetic Effects
- Entomopathogenic Microorganisms in Pest Control
- Diet, Metabolism, and Disease
- Plant and soil sciences
- Research on scale insects
Agroscope
2022-2023
Biologie Intégrative des Organismes Marins
2023
Biogen (Switzerland)
2020
Max Planck Institute for Plant Breeding Research
2012-2016
Sainsbury Laboratory
2012
Wageningen University & Research
2012
Ghent University
2012
Vlaams Instituut voor Biotechnologie
2012
University of Basel
2004-2008
Utrecht University
2007
Plants recognize pathogen-associated molecular patterns (PAMPs) via cell surface-localized pattern recognition receptors (PRRs), leading to PRR-triggered immunity (PTI). The Arabidopsis cytoplasmic kinase BIK1 is a downstream substrate of several PRR complexes. How plant PTI negatively regulated not fully understood. Here, we identify the protein phosphatase PP2C38 as negative regulator activity and BIK1-mediated immunity. dynamically associates with BIK1, well PRRs FLS2 EFR, but co-receptor...
Abstract Plasma membrane-resident receptor kinases (RKs) initiate signaling pathways important for plant immunity and development. In Arabidopsis (Arabidopsis thaliana), the elicitor-active peptide epitope of bacterial flagellin, flg22, is encoded by FLAGELLIN SENSING2 (FLS2), which promotes immunity. Despite its relevance, molecular components regulating FLS2-mediated remain largely unknown. We show that plasma membrane ARABIDOPSIS-AUTOINHIBITED Ca2+-ATPase (ACA8) forms a complex with FLS2...
• Previously we have cloned sucrose: fructan-6-fructosyltransferase (6-SFT) from barley (Hordeum vulgare) and proposed that synthesis of fructans in grasses depends on the concerted action two main enzymes: sucrose-1-fructosyltransferase (1-SST), as other fructan producing plants, 6-SFT, found only grasses. Here report cloning 1-SST, verifying activity encoded protein by expression Pichia pastoris. As expected, 1-SST is homologous to invertases fructosyltransferases, particular 6-SFT. The...
Fructans are fructose polymers that synthesized from sucrose by fructosyltransferases. Fructosyltransferases present in unrelated plant families suggesting a polyphyletic origin for their transglycosylation activity. Based on sequence comparisons and enzymatic properties, fructosyltransferases proposed to have evolved vacuolar invertases. Between 1% 5% of the total activity invertase is transglycosylating We investigated nature changes can convert hydrolysing into enzyme. Remarkably,...
Glycoside hydrolase family 32 (GH32) harbors hydrolyzing and transglycosylating enzymes that are highly homologous in their primary structure. Eight amino acids dispersed along the sequence correlated with either or glycosyltransferase activity. These were mutated onion vacuolar invertase (acINV) according to residue festuca sucrose:sucrose 1-fructosyltransferase (saSST) vice versa. acINV(W440Y) doubles transferase capacity. Reciprocally, saSST(C223N) saSST(F362Y) double hydrolysis....
A third fructan exohydrolase isoform (1‐FEHw3) was purified from wheat stems by a combination of ammonium sulfate precipitation, ConA affinity and ion‐exchange chromatography. Homogeneity the preparation indicated presence single band (70 kDa) after SDS‐PAGE. The enzyme hydrolyzed mainly β2‐1 linkages in fructans inhibited sucrose. cDNA could be obtained reverse transcriptase polymerase chain reaction (RT‐PCR)‐based strategies screening library. Functionality tests performed heterologous...
Plant fructosyltransferases are highly homologous in primary sequence and typically consist of two subunits but catalyze widely different reactions. Using functional expression the yeast Pichia pastoris , we show that substrate specificity festuca sucrose:sucrose 1‐β‐ d ‐fructosyltransferase (1‐SST) barley sucrose:fructan 6‐β‐ (6‐SFT) is entirely determined by large subunit. Chimeric enzymes with subunit 1‐SST (LSuB) small 6‐SFT have same catalytic as native vice versa. If LSuB expressed...
The active center of the glycoside hydrolase family 32 contains three characteristic motifs (N/S)DPNG, RDP, and EC. We replaced N‐terminal region including (N/S)DPNG motif barley 6‐SFT (sucrose:fructan 6‐fructosyltransferase) by corresponding Festuca 1‐SST (sucrose:sucrose 1‐fructosyltransferase). chimeric enzyme, expressed in Pichia , retained specificity 6‐SFT. Attempts to replace a larger piece at N‐terminus also RDP failed. A point mutation introduced abolished enzymatic activity....
Abstract Potato blackleg is a seedborne disease that can cause significant economic losses for growers. Disease development depends mainly on two drivers, namely seed inoculum and local climatic conditions. To better establish the relationship between these was monitored in Swiss field trials at multiple locations from 2010 to 2013 involving three sets of naturally infected lots planted each locations. The lot itself thereby most important factor explaining differences development, rather...
Plant viruses may pose a threat to crops in Colombia. To evaluate the potential risk of yield losses due plant virus infection, literature analysis followed by first field study was carried out focusing on purple passion fruit (Passiflora edulis Sims), cape gooseberry (Physalis peruviana L.), and ornamental rose (Rosa sp.), which are important Colombian exports. Over past three years, material collected from 21 farms Cundinamarca Boyacá, Colombia, two regions that close proximity El Dorado...
Tomato production is an important part of the Swiss vegetable with most tomato crops grown in greenhouses. plants are vulnerable to diseases caused by viruses, which can have significant impacts on crop production. This study reports first detection fruit botch virus (ToFBV, Blunervirus solani) Switzerland, from a site at southern Ticino region. The symptoms observed indicated presence viral pathogen, but tests against common viruses were negative. Immunocapture double-stranded RNA and its...
Sensitivity of field tests (AgriStrip and Immunochromato), DAS-ELISA, two step RT-PCR real-time for Plum pox virus (PPV) detection was tested in various tissues apricot, peach, plum damson trees infected with isolates belonging to PPV-D, PPV-M or PPV-Rec, the three strains present Slovenia. Flowers apricot full bloom proved be a very good source PPV. PPV could detected all techniques symptomatic parts leaves May one exception even beginning August, but it not asymptomatic using tests,...
Tomato bushy stunt virus (TBSV), the type member of genus Tombusvirus in family Tombusviridae is one best studied plant viruses. The TBSV natural and experimental host range covers a wide spectrum plants including agricultural crops, ornamentals, vegetables Nicotiana benthamiana. However, Arabidopsis thaliana, well-established model organism biology, genetics plant-microbe interactions absent from list known species. Most our recent knowledge life cycle has emanated studies Saccharomyces...
Ensuring the reliability of diagnostic activities is an essential cornerstone plant health strategies to reduce risk entry and spread pests in a region ultimately their impacts. Diagnostic tests should be validated ensure that they are fit for purpose. Validation usually done by laboratories, although companies commercializing kits also produce validation data products. Due high number pest, matrix, method combinations given significant resources required validate tests, it shared with...