An erratum of this article has been published full details can be found at 10.1099/jgv.0.001992 Members the family Fimoviridae are plant viruses with a multipartite negative-sense enveloped RNA genome (−ssRNA), composed 4–10 segments comprising 12.3–18.5 kb in total, within quasi-spherical virions. Fimoviruses transmitted to plants by eriophyid mites and induce characteristic cytopathologies their host plants, including double membrane-bound bodies cytoplasm virus-infected cells. Most...

Ash shoestring-associated virus (ASaV; Emaravirus fraxini) is a five-segmented, negative-sense RNA associated with chlorosis and leaf deformation in Fraxinus species. This study investigated the genetic diversity evolutionary dynamics of ASaV by analyzing nearly full-length RNA2–RNA5 partial RNA1 sequences from isolates collected different geographic regions hosts. The sequence data uncovered that has conserved genome, which RNA3 RNA5 showed more divergence than other segments sequenced...

Abstract Since Emaraviruses have been discovered in 2007 several new species were detected a range of host plants. Five genome segments novel Emaravirus from mosaic‐diseased Eurasian aspen ( Populus tremula ) completely determined. The monocistronic, segmented ssRNA the virus shows organisation typical for encoding viral RNA‐dependent RNA polymerase (RdRP, 268.2 kDa) on RNA1 (7.1 kb), glycoprotein precursor (GPP, 73.5 RNA2 (2.3 nucleocapsid protein (N, 35.6 RNA3 (1.6 and putative movement...

Emaravirus (Order Bunyavirales; Family Fimoviridae) is a genus comprising over 20 emerging plant viruses with worldwide distribution and economic impact. Emaraviruses infect variety of host plants have especially become prevalent in important long-living woody plants. These are enveloped, segmented, single-stranded, negative-sense RNA genome transmitted by eriophyid mites or mechanical transmission. four core segments encoding an RNA-dependent polymerase, glycoprotein precursor, nucleocapsid...

Viruses are frequently a microbial biocontaminant of healthy plants. The occurrence the infection can be also due to environmental stress, like urbanisation, air pollution and increased temperature, especially under ongoing climate change. aim present study was investigate hypothesis that worsened quality fewer green areas may favour higher frequency common viral infections, particularly in tree temperate continental climates, Betula pendula ROTH. We examined 18 trees, during years...

While the focus of plant virology has been mainly on horticultural and field crops as well fruit trees, little information is available viruses that infect forest trees. Utilization next-generation sequencing (NGS) methodologies revealed a significant number in trees urban parks. In present study, full-length genome novel Emaravirus identified characterized from sycamore maple ( Acer pseudoplatanus ) – tree species importance areas showing leaf mottle symptoms. RNA-Seq was performed Illumina...

Abstract We report the complete nucleotide sequence of genome a novel virus in ringspot-diseased common oak ( Quercus robur L.). The newly identified pathogen is associated with leaf symptoms such as mottle, chlorotic spots and ringspots on diseased trees. High-throughput sequencing (HTS, Illumina RNASeq) was used to explore virome that had suspected viral origin leaves for several years. Bioinformatic analysis HTS dataset followed by RT-PCR enabled us determine sequences four RNA segments...

After entry of a quarantine/regulated pathogen, infected plants shall be destroyed, and the cultivated area (e.g., greenhouse) disinfected. Therefore, selection an effective disinfectant plays important role. With availability different methods for virus quantification, we investigated application quantitative ELISA (qELISA), RT-qPCR (reverse transcription-quantitative polymerase chain reaction), bioassays quantification efficacy. estimated titer reduction in tomato brown rugose fruit...

Abstract We report the results of a virus screening conducted on selected special stands common ash ( Fraxinus excelsior ) within FraxVir project. This project among others deals with recording and assessment diversity in impact virome dieback diseased trees. Ash dieback, fungal disease caused by invasive fungus Hymenoscyphus fraxineus is major threat to populations across Europe. Over three consecutive years, trees from different origins were tested including leaf material mother seedlings...

The high prevalence of hay fever in Europe has raised concerns about the implications climate change-induced higher temperatures on pollen production. Our study focuses downy birch production across by analyzing 456 catkins during 2019-2021 37 International Phenological Gardens (IPG) spanning a large geographic gradient. As IPGs rely genetically identical plants, we were able to reduce effects genetic variability. We studied potential association with masting behavior and three model...

Abstract Fusarium proliferatum (teleomorph: Gibberella intermedia ) is a causal agent of crown rot Asparagus officinalis and one potential fumonisin‐producing species within the genus . It colonizes roots crowns asparagus plants, but could also be isolated from symptomless spears. isolates obtained perennial plantings Austria Germany were included in study on detectability variability two essential genes fumonisin‐gene cluster. Genetic fingerprinting 45 revealed 14 different fingerprint...

To unravel the virome in birch trees of German and Finnish origin exhibiting symptoms leaf-roll disease (BRLD), high-throughput sequencing (HTS) was employed. In total five viruses, among which three were so far unknown, detected by RNAseq. One to virus variants identified transcriptome individual trees. The novel viruses genetically-fully or partially-characterized, belonging genera Carlavirus, Idaeovirus Capillovirus are tentatively named carlavirus, idaeovirus, capillovirus, respectively....

Here, we report the coding-complete sequence (14,152 nucleotides [nt]) of a novel cytorhabdovirus detected in Tilia cordata and tentatively named tiliae. The assumed genome organization is 3'-N-P-P3-M-G-p6-p6'-L-5'. N gene encodes putative nucleoprotein (59.1 kDa), P phosphoprotein (34.7 P3 movement protein (23.1 M matrix G glycoprotein (64.4 L viral RNA polymerase (247 kDa). P6 P6' are overlapping open reading frames (ORFs), which may encode products 7.9 9.5 kDa, respectively, unknown functions.

High-altitude environments are highly susceptible to the effects of climate change. Thus, it is crucial examine and understand behaviour specific plant traits along altitudinal gradients, which offer a real-life laboratory for analysing future impacts The available information on how pollen production varies at different altitudes in mountainous areas limited. In this study, we investigated 17 birch (Betula pubescens Ehrh.) individuals an gradient European Alps. We sampled catkins nine...

Abstract The occurrence of Fusarium spp. and associated mycotoxins in asparagus spears was evaluated Poland 2002 2003 Germany 2002. Spears two cultivars, Eposs Gijnlim, were collected from locations Poland, Swidwowiec Poznan, on sandy loam soil, respectively. oxysporum F. proliferatum detected at an average incidence 38.3% 15.8% the spear sections sampled, In stands 11 (tested) cultivars sampled same species dominated, however, they less frequent than (26.6% 5.6% infected with ,...

We observed the health status of oak trees in a conservation seed orchard for over twenty years, focusing on characteristic virus-suspected symptoms. The was established 1992 Kreuztal, North Rhine-Westphalia (Germany) with 1302 seedlings 186 clusters. number showing chlorotic ringspots and mottle leaves has fluctuated annually, but increased from 3.3% to 12.1% last 20 years; affected clusters risen 8% 25.9%. A scientific breakthrough identification novel virus related members genus...

We identified a novel virus in diseased European ash (Fraxinus excelsior) and manna (F. ornus) trees exhibiting chlorotic ringspots, mottle leaf deformation such as curling shoestring symptoms. High-throughput sequencing (HTS, Illumina RNASeq) of total RNA isolated from material combination with RT-PCR-based amplification techniques Sanger determined five complete genome segments, each encoding single open reading frame. Sequence analyses RNA1–RNA5 revealed organization typical for...

<ja:p>Cherry leaf roll virus, CLRV, is a plant pathogen that infects variety of deciduous trees and shrubs in temperate regions. Little known about its occurrence at high latitudes especially Finnish birch species. Still, symptoms seemed to be associated with CLRV such as vein banding, decline have been observed throughout the country since summer 2002. Six different species, subspecies or varieties, i.e. Betula pubescens subsp. (downy birch), B. pendula (silver nana (dwarf var. appressa...

The European mountain ash ringspot-associated virus (EMARaV) is a multipartite RNA of negative polarity. It infects Sorbus aucuparia (common name—rowan) trees throughout their whole distribution area in North and Central Europe. causes mottling, chlorotic ringspots decline the plant. Infected rowans are serious sources for other potential hosts. EMARaV incidence population structure was investigated Germany, Finland, Sweden, Scotland, Norway. Overall, variants from 42 rowan distributed 20...