A5054097099- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Plant Pathogens and Resistance
- Entomopathogenic Microorganisms in Pest Control
- Potato Plant Research
- Natural product bioactivities and synthesis
- Plant-Microbe Interactions and Immunity
- Insect Pest Control Strategies
- Marine Invertebrate Physiology and Ecology
- Plant Disease Management Techniques
- Plant Pathogenic Bacteria Studies
- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- Marine Ecology and Invasive Species
- Microbial Metabolites in Food Biotechnology
- Dengue and Mosquito Control Research
- Polyamine Metabolism and Applications
- Plant Reproductive Biology
- Phytoplasmas and Hemiptera pathogens
- Animal testing and alternatives
- Cassava research and cyanide
- Insect and Arachnid Ecology and Behavior
- Agricultural safety and regulations
- Glycosylation and Glycoproteins Research
Agroscope
2019-2024
Vifor Pharma (Switzerland)
2022-2024
KTH Royal Institute of Technology
2006-2017
AlbaNova
2017
Stockholm University
2013-2017
Pacific Biosciences (United States)
2012
Understanding the functional relationship between intracellular factors and extracellular signals is required for reconstructing gene regulatory networks (GRN) involved in complex biological processes. One of best-studied bilaterian GRNs describes endomesoderm specification predicts that both mesoderm endoderm arose from a common GRN early animal evolution. Compelling molecular, genomic, developmental, evolutionary evidence supports hypothesis bifunctional gastrodermis cnidarian-bilaterian...
The plant parasitic root-knot nematodes Meloidogyne spp. are a devastating threat to agriculture. urgent need search for alternative nematode control methods that less environmentally toxic is demanding challenge secure the increasing global food demand. Therefore, we combined chemical and biological strategies evaluate their management potential on incognita infested tomatoes. To determine effect of nematicides Velum Purpureocillium lilacinum strain 251 formulated as wettable granulate...
The excessive application of chemical fertilizers in intensively managed agricultural fields worldwide has resulted soil degradation and biodiversity loss.This contributed to a growing interest sustainable management, such as organic farming.Until now, studies addressing the impact conventional management on functioning have mainly focused arable farming only few reports are available vegetable production.Vegetable is particular interest, since intensity usually very high there an increasing...
The oomycete class includes pathogens of animals and plants which are responsible for some the most significant global losses in agriculture aquaculture. There is a need to replace traditional chemical means controlling growth with more targeted approaches, inhibition sterol synthesis one promising area. To better direct these efforts, we have studied acquisition two model organisms: sterol-autotrophic Saprolegnia parasitica, sterol-heterotrophic Phytophthora infestans. We first present...
Sterols play a key role in various physiological processes of plants. Commonly, stigmasterol, β-sitosterol and campesterol represent the main plant sterols, cholesterol is often reported as trace sterol. Changes especially β-sitosterol/stigmasterol levels, can be induced by different biotic abiotic factors. Plant parasitic nematodes, such root-knot nematode Meloidogyne incognita, are devastating pathogens known to circumvent defense mechanisms. In this study, we investigated changes sterols...
The devastating root-knot nematode
Abstract Backbone N-methylation and macrocyclization improve the pharmacological properties of peptides by enhancing their proteolytic stability, membrane permeability target selectivity. Borosins are backbone N-methylated peptide macrocycles derived from a precursor protein which contains α-N-methyltransferase domain autocatalytically modifying core located at its C-terminus. Founding members borosins omphalotins mushroom Omphalotus olearius (omphalotins A-I) with nine out 12 L -amino acids...
Returning biogas digestate to the fields as biofertilizer is an established agricultural practice which has been promoting plant resistance soil borne pests. Root-knot nematodes (Meloidogyne spp.) are widely distributed Plant Parasitic Nematodes (PPNs), constituting a major threat world's food supply. Novel PPNs control methods needed in context of sustainable agriculture replace or complement use hazardous synthetic nematicides. The identification nematicidal compounds digester effluents...
Steroidal glycoalkaloids (SGAs) are plant secondary metabolites known to be toxic animals and humans that have putative roles in defense against pests. The proposed mechanisms of SGA toxicity sterol-mediated disruption membranes inhibition cholinesterase activity neurons. It has been suggested phytopathogenic microorganisms can overcome by enzymatic deglycosylation SGAs. Here, we explored SGA-mediated toward the invasive oomycete Phytophthora infestans, causative agent late blight disease...
Biofumigation, although a well-known method, is still controversially debated as management strategy for plant-parasitic nematodes (PPN). Its controlling effect attributed to the production of isothiocyanates (ITCs) following action myrosinase on glucosinolates (GSLs). Different ITCs are formed from different GSLs, depending plant species. To better understand potential ITCs, eight cultivars three Brassicaceae species were investigated biofumigation crops control root knot nematode...
VALITEST is an EU‐funded project built to improve the reliability of diagnostic tests performed in plant health laboratories across European and Mediterranean region. The undertaken by a consortium 16 partners composed research institutions, private companies (such as kit providers), national protection organizations one intergovernmental organization (EPPO). Current harmonized procedures for validation test performance studies will be improved based on experience gained from including...
Sterols are isoprenoid-derived lipids that play essential structural and functional roles in eukaryotic cells. Plants produce a complex mixture of sterols, changes plant sterol profiles have been linked to plant-pathogen interactions. β-Sitosterol stigmasterol, particular, associated with defense. As nematodes lost the ability synthesize sterols de novo, they require from host. Tomato (Solanum lycopersicum) plants infected by parasitic nematode Meloidogyne incognita show reduced level...
Steroidal glycoalkaloids (SGA) are toxic secondary metabolites naturally occurring in the potato, as well certain other Solanaceous plant species, such tomato, eggplant and pepper. To investigate steroidal origin of SGA biosynthesis, cut potato shoots were fed cholesterol labelled with deuterium (D) sterol ring structure (D5- or D6-labelled), side chain (D7-labelled), analysed after three five weeks. The presence D-labelled by GC-MS LC-MS/MS, respectively. When feeding solubilised Tween-80,...
Coprinopsis cinerea lectin 2 (CCL2) is a fucoside-binding from the basidiomycete C. that toxic to bacterivorous nematode Caenorhabditis elegans as well animal-parasitic and fungivorous nematodes. We expressed CCL2 in Arabidopsis assess its protective potential toward plant-parasitic Our results demonstrate expression of enhances host resistance against cyst Heterodera schachtii. Surprisingly, CCL2-expressing plants were also more resistant fungal pathogens including Botrytis cinerea,...
From 2016 to 2021, nematode surveys in Florida strawberry fields revealed several species of foliar nematodes (
Abstract Aims Clonostachys rosea is a well-known mycoparasite that has recently been investigated as bio-based alternative to chemical nematicides for the control of plant-parasitic nematodes. In search promising biocontrol agent, ability C. strain PHP1701 southern root-knot nematode Meloidogyne incognita was tested. Methods and Results Control M. in vitro soil by significant concentration dependent. Small pot greenhouse trials confirmed reduction tomato root galling compared untreated...
Research into new nematicides that provide adequate control against root-knot nematodes in a more environment-friendly way is of great interest to vegetable growers. Thus, the effect fluopyram, relatively chemical nematicide, was evaluated Swiss population Meloidogyne incognita vitro, soil and as planting hole treatment for tomato, lettuce cucumber plants. Fluopyram vitro revealed LC50 (lethal concentration, 50%) ranging from 2.15–0.04 µmol fluopyram/L after 1–14 days exposure. However, some...
The first committed step of sterol biosynthesis is the cyclisation 2,3-oxidosqualene to form either lanosterol (LA) or cycloartenol (CA). This catalyzed by an oxidosqualene cyclase (OSC). LA and CA are subsequently converted into various sterols a series enzyme reactions. specificity OSC therefore determines final composition end organism. Despite functional importance OSCs, determinants their not well understood. In sterol-synthesizing oomycetes, recent bioinformatics, metabolite analysis...
Globodera rostochiensis and G. pallida are some of the most successful highly specialized plant parasitic nematodes among regulated quarantine pests globally. In Switzerland, they have been monitored by annual surveys since their first detection in Swiss soil 1958. The dataset created was reviewed to produce an overview development actual status potato cyst (PCNs) Switzerland. Positive fields represent 0.2% all samples analyzed, distribution is limited central-west western suggesting that...
Foliar nematodes represent a minor feeding group within the genus Aphelenchoides Fischer, 1894. The facultative plant parasitic species A. blastophthorus can cause crinkling of leaves, reduced vigor, and stunting agricultural ornamental plants. Here we report first finding in crowns, roots strawberry plants collected Switzerland 2018. Species identification was confirmed by morphological morphometric characterization supported molecular barcoding 18S ribosomal RNA (18S), 28S (28S),...
Abstract Phytonematode infections are a major constraint to agriculture and can cause pre-harvest losses of up one-third the crop in affected fields. With increasing restrictions on use chemical pesticides due environmental human hazards, nematode pressure climate change, soil degradation agricultural intensification, more sustainable ways manage such plant pests needed meet growing demand for food. Therefore, reproduction thermophilic Meloidogyne spp. was evaluated comparison with that...
Plant-parasitic nematodes and herbivorous insects have a significant negative impact on global crop production. A successful approach to protect crops from these pests is the in planta expression of nematotoxic or entomotoxic proteins such as crystal Bacillus thuringiensis (Bt) plant lectins. However, efficacy this threatened by emergence resistance nematode insect populations proteins. To solve problem, novel are needed. During last two decades, several cytoplasmic lectins mushrooms with...