A5044089291- Synthetic Organic Chemistry Methods
- Microbial Natural Products and Biosynthesis
- Monoclonal and Polyclonal Antibodies Research
- Traditional and Medicinal Uses of Annonaceae
- Glycosylation and Glycoproteins Research
- Weed Control and Herbicide Applications
- Antifungal resistance and susceptibility
- Synthesis and Biological Activity
- Carbohydrate Chemistry and Synthesis
- Synthesis and biological activity
- Nematode management and characterization studies
- Pesticide Residue Analysis and Safety
- Pesticide and Herbicide Environmental Studies
- Click Chemistry and Applications
- Marine Sponges and Natural Products
- Sirtuins and Resveratrol in Medicine
- Lymphoma Diagnosis and Treatment
- Synthesis of Organic Compounds
- Asymmetric Synthesis and Catalysis
- Synthesis and Biological Evaluation
- Botany, Ecology, and Taxonomy Studies
- Fungal Biology and Applications
- Fungal Plant Pathogen Control
- Cancer Treatment and Pharmacology
- Crystallization and Solubility Studies
Agroscope
2016-2025
National Center for Tumor Diseases
2010-2025
Ludwig-Maximilians-Universität München
2011-2024
University of Würzburg
1999-2021
Universitätsklinikum Würzburg
2007-2021
Comprehensive Cancer Center Mainfranken
2021
Klinik und Poliklinik für Neurologie
2018
Max Rubner Institut
2018
Heidelberg University
2008-2016
University Hospital Heidelberg
2008-2016
The plant parasitic root-knot nematodes Meloidogyne spp. are a devastating threat to agriculture. urgent need search for alternative nematode control methods that less environmentally toxic is demanding challenge secure the increasing global food demand. Therefore, we combined chemical and biological strategies evaluate their management potential on incognita infested tomatoes. To determine effect of nematicides Velum Purpureocillium lilacinum strain 251 formulated as wettable granulate...
ABSTRACT Herpes simplex virus (HSV) glycoprotein B (gB) is an integral part of the multicomponent fusion system required for entry and cell-cell fusion. Here we investigated mechanism viral neutralization by monoclonal antibody (MAb) 2c, which specifically recognizes gB HSV type 1 (HSV-1) HSV-2. Binding MAb 2c to a type-common discontinuous epitope resulted in highly efficient at postbinding/prefusion stage completely abrogated cell-to-cell spread vitro . Mapping antigenic site recognized...
Abstract Resveratrol as well caloric restriction were shown to extend lifespan in some model organisms and may possibly delay onset of ageing-related diseases humans. Yet, resveratrol supplementation does not always animal models or improve health status Because interindividual differences human microbiota, metabolite production the gut differs. While individuals produce lunularin dihydroresveratrol their gut, others only. Therefore, we addressed question whether these metabolites differ...
The devastating root-knot nematode
Background CV8102, a toll-like receptor 7/8 and RIG I agonist, has demonstrated antitumor immune responses in preclinical studies. We investigated intratumoral (IT) administration of CV8102 patients with anti-programmed cell death protein-1 (PD-1) therapy-naïve or anti-PD-1 therapy-refractory cutaneous melanoma (cMEL) advanced squamous carcinoma, head neck carcinoma adenoid cystic carcinoma. Methods This open-label, cohort-based, phase dose escalation study aimed to establish the maximum...
The herbicide asulam is a weakly adsorbing and, thus, mobile substance. However, due to its rapid degradation, leaching groundwater not expected based on simulations with soil models used predict pesticide concentrations in as part of the authorization process. Asulam was, nevertheless, frequently detected samples from 112 monitoring sites Switzerland, albeit at low up 0.017 μg/L. A potential input pathway for systemic includes processes uptake by leaves treated weeds, translocation roots,...
Antibody-drug conjugates (ADCs) have evolved as a new class of potent cancer therapeutics. We here report on the development ADCs with specificity for B-cell lineage specific (surface) antigen CD22 being expressed in majority hematological malignancies. As targeting moiety previously generated humanized anti-CD22 single-chain variable fragment (scFv) derivative from monoclonal antibody RFB4 was reengineered into IgG1 format (huRFB4). Onconase (ranpirnase), clinically active pancreatic-type...
Metabolism of chiral pesticides in crops is typically studied using achiral analytical methods and, consequently, the stereoisomer composition residues unknown. In this study, we developed an enantioselective GC-MS/MS method to quantify fungicides fenpropidin, fenpropimorph, and spiroxamine plant matrices. field trials, were applied grapevines, sugar beets, or wheat. Fenpropidin was metabolized with no only weak enantioselectivity. For slightly metabolism observed wheat but more pronounced...
Abstract Aims Clonostachys rosea is a well-known mycoparasite that has recently been investigated as bio-based alternative to chemical nematicides for the control of plant-parasitic nematodes. In search promising biocontrol agent, ability C. strain PHP1701 southern root-knot nematode Meloidogyne incognita was tested. Methods and Results Control M. in vitro soil by significant concentration dependent. Small pot greenhouse trials confirmed reduction tomato root galling compared untreated...
A prerequisite for the enrichment of antibodies screened from phage display libraries is their stable expression on a during multiple selection rounds. Thus, if stringent panning procedures are employed, simultaneously driven by antigen affinity, stability and solubility. To take advantage robust pre‐selected scaffolds such molecules, we grafted single‐chain Fv (scFv) antibodies, previously isolated human library after rounds in vitro tumor cells, with specificity clinically established...
Platensimycin is a natural product isolated from various strains of Streptomyces platensis which exhibits antimicrobial activity against Gram positive bacteria, including vancomycin- and linezolide-resistant species. Analogues platensimycin were synthesized 3-aminobenzoic acid or other aniline derivatives several alkyl- aryl-carboxylic acids. The resulting compounds tested in an agar diffusion assay bacteria fungi.
A series of N ‐alkyl trans ‐decahydroisoquinoline, 1,2,3,4‐tetrahydroisoquinoline, and 6,7‐dimethoxy‐1,2,3,4‐tetrahydroisoquinoline derivatives were synthesized starting from the respective secondary amines by ‐alkylation with alkyl bromides. The compounds C 11 chains showed antifungal potency comparable to clotrimazole, inhibit enzymes ergosterol biosynthesis (Δ14‐reductase Δ8,7‐isomerase), depending on heterocyclic scaffold investigated species.
Abstract The generation of a single chain Fv (scFv) fragment derived from the anti‐CD22 monoclonal antibody LL2 resulted in molecule with good antigen binding but very poor stability properties, thus hampering its clinical applicability. Here we report on construction an engineered scFv by rational mutagenesis. contribution uncommon wild‐type sequence residues for providing to conserved common core structure immunoglobulins was examined. Aided computer homology modeling, 3 destabilizing...
Background: Periinsular hemispherotomy is used to treat catastrophic epilepsy in hemispheric lesions. It avoids complications of tissue resection performed other hemispherectomy procedures. We report on the effectiveness, complications, and neurological sequelae patients with Sturge-Weber syndrome (SWS). Methods: Eight (mean age at operation 15 months, mean surveillance time 7.1 years, 5 girls) were operated between 1994 2000, one additional subpial resections insular cortex. Preoperatively...
Benzylamine‐type antimycotics like naftifine, butenafine, or terbinafine are a well‐known class of since the 1980s. The following paper describes synthesis and biological evaluation series novel benzylamine‐type characterized by an isooctyl side chain various substituents at benzylamine moiety. compounds were prepared from benzaldehyde derivatives 2‐amino‐6‐methylheptane reductive amination with sodium triacetoxyborohydride subsequent precipitation hydrogen chloride. antimycotic activity...
Abstract BACKGROUND The application of plant protection products (PPPs) leads to the formation residues in treated crops. Even though PPPs contain considerable amounts co‐formulants, regulation and monitoring normally focus on active substances (a.s.) only. For our study we selected four commonly used co‐formulants (three anionic surfactants one organic solvent) investigated decline vegetables apples under field conditions. aims were characterize behavior co‐formulant crops provide a basis...
Abstract A series of 23 novel benzylamines was synthesized by reductive amination from halogen‐substituted 3‐ and 4‐benzyloxybenzaldehyde derivatives 6‐methylhept‐2‐yl amine or n ‐octylamine. The antimycotic activity the resulting amines evaluated in a microdilution assay against apathogenic yeast Yarrowia lipolytica as test microorganism. Promising compounds were also tested human pathogenic Candida species. influence halogen substituents at benzyl ether side chain studied this screening,...
By varying linker length and domain orientation three multivalent derivatives of a monovalent anti‐CD22 single‐chain fragment variable (scFv) antibody were generated. Shortening the V H –V L oriented scFv to 5 or 0 residues resulted in formation diabodies mixture tetramers trimers, respectively. Unexpectedly, –0–V assembled homogenous dimers, remained substantially more stable than –5–V diabody when incubated human serum at 37 °C, retained its dimeric state concentrated up 4 mg/ml. These...
Cutaneous markers in the lumbo-sacral region are indicators of occult spinal dysrhaphism and tethered cord. By means sonography, anatomical abnormalities cord can be shown neonatal period.We report on 6 infants with cutaneous who were investigated a high resolution linear transducer (> 7.5 MHz) computer sonographic unit (Sequoia, Acuson). The investigations performed between first day life ninth week (m: 26 days). following could found: Asymmetrical gluteal crease (4); dermal sinus (2),...