A5062310114- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Phytoplasmas and Hemiptera pathogens
- Banana Cultivation and Research
- Entomopathogenic Microorganisms in Pest Control
- Cocoa and Sweet Potato Agronomy
- Legume Nitrogen Fixing Symbiosis
- Garlic and Onion Studies
- Scientific Measurement and Uncertainty Evaluation
- Microbial infections and disease research
- Plant Virus Research Studies
- Cassava research and cyanide
- Plant Parasitism and Resistance
- Microbial Metabolic Engineering and Bioproduction
- Pesticide Residue Analysis and Safety
- Insect-Plant Interactions and Control
- Plant and Fungal Species Descriptions
- Microbial Community Ecology and Physiology
- Research on scale insects
- Plant Disease Resistance and Genetics
- Medical Imaging Techniques and Applications
- Forest Insect Ecology and Management
- Metal Extraction and Bioleaching
- African Botany and Ecology Studies
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2015-2022
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2021-2022
Advanced Network and Services (United States)
2019-2021
Peuplements végétaux et bioagresseurs en milieu tropical
2009-2016
Département Santé des Plantes et Environnement
2013-2015
Copper-based antimicrobial compounds are widely used to control plant bacterial pathogens. Pathogens have adapted in response this selective pressure. Xanthomonas citri pv. citri, a major citrus pathogen causing Asiatic canker, was first reported carry plasmid-encoded copper resistance Argentina. This phenotype conferred by the copLAB gene system. The emergence of resistant strains has since been Réunion and Martinique. Using microsatellite-based genotyping PCR, we demonstrated that genetic...
Given the risk of zoonotic disease emergence, including new SARS-CoV-2 variants COVID-19, rapid diagnostic tools are urgently needed to improve control spread infectious diseases. A one-pot triplex real-time RT-LAMP (reverse-transcription-loop-mediated isothermal amplification) assay, based on two regions genome SARS-CoV-2-specifically Orf1ab and N genes-along with one internal control, human RNase P gene, was developed. The multiplexing relies distinct melting peaks produced during an...
VALITEST is an EU‐funded project built to improve the reliability of diagnostic tests performed in plant health laboratories across European and Mediterranean region. The undertaken by a consortium 16 partners composed research institutions, private companies (such as kit providers), national protection organizations one intergovernmental organization (EPPO). Current harmonized procedures for validation test performance studies will be improved based on experience gained from including...
Banana wilt outbreaks that are attributable to Moko disease-causing strains of the pathogen Ralstonia solanacearum (Rs) remain a social and economic burden for both multinational corporations subsistence farmers. All known belong phylotype II lineage, which has been previously recognized its broad genetic basis. paraphyletic distributed among seven related but distinct phylogenetic clusters (sequevars) potentially major threats Musaceae, Solanaceae, ornamental crops in many countries....
Abstract Background Asiatic Citrus Canker, caused by Xanthomonas citri pv. citri, severely impacts citrus production worldwide and hampers international trade. Considerable regulatory procedures have been implemented to prevent the introduction establishment of X. into areas where it is not present. The effectiveness this surveillance largely relies on availability specific sensitive detection protocols. Although several PCR- or real-time PCR-based methods are available, most them showed...
The aim of this study was to characterise the performance new molecular methods for detection and identification Pseudomonas syringae pv. actinidiae (Psa) provide validation data in comparison assays mentioned official diagnostic protocols being currently used. Eleven tests Psa were compared an inter-laboratory where each laboratory had analyse same panel samples consisting thirteen Psa-spiked kiwifruit wood extracts. Laboratories perform also isolation from Data interlaboratory test (TPS)...
A working group established in the framework of EUPHRESCO European collaborative project aimed to compare and validate diagnostic protocols for detection "Flavescence dorée" (FD) phytoplasma grapevines. Seven molecular were compared an interlaboratory test performance study where each laboratory had analyze same panel samples consisting DNA extracts prepared by organizing laboratory. The tested methods consisted universal group-specific real-time end-point nested PCR tests. Different...
Abstract Appropriate statistical analysis of the validation data for diagnostic tests facilitates evaluation performance criteria and increases confidence in conclusions drawn from these data. A comprehensive approach to analysing reporting studies inter‐laboratory comparisons such as test is described. The proposed methods, including analyses, presentation interpretation data, are illustrated using a real dataset generated during study conducted framework European project, VALITEST. This...
Efficient control of Xanthomonas axonopodis pv. dieffenbachiae , the causal agent anthurium bacterial blight, requires sensitive and reliable diagnostic tools. The European standard EN ISO 16140:2003 has been followed to compare a nested PCR assay (N‐ ) reference method (isolation serological identification colonies) other alternative detection methods. evaluation was performed in two steps: comparative study collaborative involving 15 laboratories. Although inclusivity maximal (100%) for...
Xanthomonas axonopodis pv. dieffenbachiae causes anthurium blight, which is regarded as the most threatening disease for industry worldwide. The bacterium listed a quarantine pathogen in several regions, including Europe. We evaluated use of Neomycin-Cephalexin-Trimethoprime-pirMecillinam 4 (NCTM4) medium its isolation.A total 104 bacterial strains were inoculated onto NCTM4 and on previously published Cellobiose-Starch (CS) Esculin-Trehalose (ET) media. strain collection included: blight...
The use of methods analysis capable producing reliable analytical results is a prerequisite to the effective control quarantine plant pathogens. Proficiency testing considered be one most ways verify and coordinate results. As French national reference laboratory in pathology, Anses Plant Health Laboratory organizes proficiency tests order ensure that officially approved laboratories (certified by government services) are for detection pathology have number notable features including...
Ensuring the reliability of diagnostic activities is an essential cornerstone plant health strategies to reduce risk entry and spread pests in a region ultimately their impacts. Diagnostic tests should be validated ensure that they are fit for purpose. Validation usually done by laboratories, although companies commercializing kits also produce validation data products. Due high number pest, matrix, method combinations given significant resources required validate tests, it shared with...