A5086405936- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Phytoplasmas and Hemiptera pathogens
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- Cocoa and Sweet Potato Agronomy
- Insect-Plant Interactions and Control
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Agronomic Practices and Intercropping Systems
- Microbial Natural Products and Biosynthesis
- Soybean genetics and cultivation
- Horticultural and Viticultural Research
- Mycorrhizal Fungi and Plant Interactions
- Plant-Microbe Interactions and Immunity
- Nematode management and characterization studies
- Essential Oils and Antimicrobial Activity
- Potato Plant Research
- Cassava research and cyanide
- Metabolomics and Mass Spectrometry Studies
- Insect symbiosis and bacterial influences
- Microbial Metabolic Engineering and Bioproduction
- Hymenoptera taxonomy and phylogeny
- Mycotoxins in Agriculture and Food
Agroscope
2014-2025
Plant Production Research Institute
2014
Gymnase de Nyon
2014
University of Geneva
2005-2010
The co-cultivation of fungi has recently been described as a promising strategy to induce the production novel metabolites through possible gene activation. A large screening fungal co-cultures in solid media identified an unusual long-distance growth inhibition between Trichophyton rubrum and Bionectria ochroleuca. To study metabolite induction this particular interaction, differential LC-MS-based metabolomics was performed on pure strain cultures their co-cultures. comparison resulting...
Summary A type III protein secretion system (T3SS) is an important host range determinant for the infection of legumes by Rhizobium sp. NGR234. Although a functional T3SS can have either beneficial or detrimental effects on nodule formation, only rhizobial‐specific positively acting effector proteins, NopL and NopP, been characterized. NGR234 possesses three open reading frames potentially encoding homologues proteins from pathogenic bacteria. NopJ, NopM NopT are secreted All negative...
Massive outbreaks of virus yellows (VY) and syndrome "basses richesses" (SBR) are thought to be responsible for the major loss sugar beet yields in 2020 western cantons Switzerland. Typical yellowing symptoms were visible during field inspections, control measures reportedly ineffective or even absent. Both diseases induce but have distinct etiologies; while VY is caused by aphid-transmitted RNA viruses, SBR cixiid-transmitted γ-proteobacterium Candidatus Arsenophonus phytopathogenicus. To...
We report the characterization of a novel tri-segmented RNA virus infecting Mercurialis annua, common crop weed and model species in plant science. The virus, named "Mercurialis latent virus" (MeLaV) was first identified mixed infection with recently described orthotospovirus 1 (MerV1) on symptomatic plants grown glasshouses Lausanne (Switzerland). Both viruses were found to be transmitted by Thrips tabaci, which presumably help inoculation infected pollen case MeLaV. Complete genome...
Abstract The phloem-restricted phytopathogenic bacterium Candidatus Arsenophonus phytopathogenicus (Ap) causes the “syndrome basses richesses” (SBR), which has recently emerged as a major burden for sugar beets in several countries of Western Europe. Here, we report first identification Ap and its planthopper vector Pentastiridius leporinus potato fields Switzerland 2023. was detected plants tubers exhibiting phytoplasma-like symptoms collected from cantons currently experiencing SBR...
ABSTRACT Bacteria infecting the plant phloem represent a growing threat worldwide. While these organisms often resist in vitro culture, they multiply both sieve elements and hemipteran vectors. Such cross-kingdom parasitic lifestyle has emerged diverse taxa via distinct ecological routes. In genus Arsenophonus , pathogens “ Candidatus phytopathogenicus” (Ap) Ca . Phlomobacter fragariae” (Pf) have evolved from insect endosymbionts, but genetic mechanisms underlying this transition not been...
A multi-well approach was developed for time series studies of <italic>de novo</italic> metabolite-induction by fungal co-culture using untargeted metabolomics.
Members of the genus 'Candidatus Phytoplasma' are obligate intracellular bacteria restricted to phloem sieve elements and able colonize several tissues hemolymph in their insect vectors. The current unfeasibility axenic culture low complexity genomic sequences obstacles assembling complete chromosomes. Here, a method combining pathogen DNA enrichment from infected insects dual deep-sequencing technologies was used obtain genome phytoplasma causing Grapevine Flavescence dorée. de novo...
Grapevine red blotch virus (GRBV) is a recently described that infects grapevine. Little information available on the possible occurrence and distribution outside North America. Therefore, we surveyed commercial vineyards from three major grape-growing regions in Switzerland to determine presence or absence of GRBV. In total, 3,062 vines were analyzed by polymerase chain reaction. None tested positive for GRBV, suggesting GRBV Swiss vineyards. We also investigated whether was present 653...
Potato virus Y (PVY) is considered the most economically damaging for seed-potato production. PVY isolates are usually divided into three historical strains, namely PVYC, PVYO and PVYN. More recently, recombinant strains named PVYNTN PVYN-Wi appeared in Europe North America, rapidly spread production gradually replaced strains. This progression can be explained by several factors, including differential susceptibility of cultivars to article presents results a Swiss survey conducted 2012...
Leaves of hollyhock (Alcea rosea) exhibiting vein chlorosis and yellow mosaic symptoms were collected at public sites in Lausanne Nyon, two cities western Switzerland. Diagnostic methods untangled samples from both the mixed infections a novel isometric virus, tentatively named "Alcea virus" (AYMV) with carlavirus Gaillardia latent virus. A new potyvirus was also identified Nyon. combination Illumina, Nanopore Sanger sequencing necessary to assemble full-length genome AYMV, revealing an...
Abstract ‘Flavescence dorée’ (FD) is a quarantine grapevine disease associated with FD phytoplasmas (FDp). In Switzerland, was first reported in 2004 the southern part of Canton Ticino, from where, within few years, it spread to nearly entire vineyard area Southern Alps, despite mandatory control measures. The aim this study assess possible role gone‐wild grapevines (GWGVs) forests resulting early abandoned vineyards as habitat for main FDp vector, Scaphoideus titanus , and best alternative...
The ability of fungi isolated from nails patients suffering onychomycosis to induce de novo production bioactive compounds in co-culture was examined. Comparison between the metabolite profiles produced by Sarocladium strictum, Fusarium oxysporum, and these two species revealed induction fusaric acid based on HRMS. Structure confirmation this toxin, using sensitive microflow NMR, required only three 9-cm Petri dishes fungal culture. A targeted metabolomics study UHPLC-HRMS confirmed that...
High-throughput sequencing (HTS), more specifically RNA of plant tissues, has become an indispensable tool for virologists to detect and identify viruses. During the data analysis step, typically compare obtained sequences reference virus databases. In this way, they are neglecting without homologies viruses, which usually represent majority reads. We hypothesized that traces other pathogens might be detected in unused sequence data. present study, our goal was investigate whether total...
In plants, RNA silencing–based antiviral defense generates viral small RNAs (sRNAs) faithfully representing the genomes. We employed sRNA sequencing and bioinformatics (sRNA-omics) to characterize reconstruct full genomic sequences their variants in evolving quasispecies cultivated solanaceous plants carrying mixed infections. naturally infected Solanum tuberosum (potato), one case study revealed a virome comprising Potato virus Y (genus Potyvirus) X Potexvirus), which was reconstructed by...
In Switzerland, greenhouses for tomato (Solanum lycopersicum) production are inspected systematically Tomato brown rugose fruit virus (ToBRFV) in accordance with phytosanitary regulations, by sampling symptomatic plants during routine visits. July 2021, a suspected viral disease was reported from an 8-hectare greenhouse the canton of Thurgau eastern on single plant soil-grown crop (cv. Dubino). This exhibited light discoloured spots which were unusual this variety and rather reminiscent...
Recent developments in high-throughput sequencing (HTS) technologies and bioinformatics have drastically changed research virology, especially for virus discovery. Indeed, proper monitoring of the viral population requires information on different isolates circulating studied area. For this purpose, HTS has greatly facilitated new genomes detected viruses their comparison. However, analyses allowing reconstruction genome sequences detection single nucleotide polymorphisms (SNPs) can...
Every year, Agroscope examines nearly 300,000 tubers for the presence of viruses, as regulated certification seed potatoes intended Swiss growers. Since 2016, this examination has been performed via RT-qPCR on dormant directly after harvest. This method offers fast results and eliminates need use Rindite, which is a toxic polluting gaseous compound previously used in Switzerland to break dormancy tubers. The implementation molecular analytical routine diagnosis viruses makes it possible...
Abstract Grapevine red blotch virus (GRBV) is a recently identified that infects grapevine and has severe impact on the grape industry in North America. Since first description of 8 years ago, clear progress been made regarding our understanding GRBV pathosystem. However, questions remain origin this pathogen its spread outside America, especially Europe. In study, we present results large-scale survey two European repositories; targeted Vitis spp. accessions with diverse geographical...