A5052094213- Legume Nitrogen Fixing Symbiosis
- Peptidase Inhibition and Analysis
- Plant nutrient uptake and metabolism
- Biochemical and Structural Characterization
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- Plant responses to water stress
- Biochemical and Molecular Research
- Studies on Chitinases and Chitosanases
- Protease and Inhibitor Mechanisms
- Metal-Catalyzed Oxygenation Mechanisms
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolites in Food Biotechnology
- Bacterial Genetics and Biotechnology
- Agronomic Practices and Intercropping Systems
- Bacteriophages and microbial interactions
- Trace Elements in Health
Aarhus University
2012-2020
LysM domains, which are frequently present as repetitive entities in both bacterial and plant proteins, known to interact with carbohydrates containing N -acetylglucosamine (GlcNAc) moieties, such chitin peptidoglycan. In bacteria, the functional significance of involvement multiple domains substrate binding has so far lacked support from high-resolution structures ligand-bound complexes. Here, a structural study Thermus thermophilus NlpC/P60 endopeptidase two is presented. The crystal...
Abstract Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition responses to microbial associated molecular patterns in mammals plants. In legumes, EPS are monitored can either block or promote symbiosis with rhizobia depending on their composition. To establish a deeper understanding receptors involved recognition, we determined the structure Lotus japonicus ( ) exopolysaccharide receptor 3 (EPR3)...
The establishment of nitrogen-fixing root nodules in legume-rhizobia symbiosis requires an intricate communication between the host plant and its symbiont. We are, however, limited our understanding signaling process. In particular, how membrane-localized receptors legumes activate signal transduction following perception rhizobial molecules has mostly remained elusive. To address this, we performed a coimmunoprecipitation-based proteomics screen to identify proteins associated with Nod...
The oxidation resistance proteins (OXR) help to protect eukaryotes from reactive oxygen species. sole C-terminal domain of the OXR, named TLDc is sufficient perform this function. However, mechanism by which occurs poorly understood. We present here crystal structure protein 2 zebrafish. was determined X-ray crystallography atomic resolution (0.97Å) and adopts an overall globular shape. Two antiparallel β-sheets form a central β-sandwich, surrounded two helices one-turn helices. fold shares...
Cellulose, chitin and peptidoglycan are major long-chain carbohydrates in living organisms, constitute a substantial fraction of the biomass. Characterization biochemical basis dynamic changes degradation these β,1-4-linked is therefore important for both functional studies biological polymers biotechnology. Here, we investigated role multiplicity carbohydrate-binding lysin motif (LysM) domain that found proteins involved bacterial synthesis remodelling. The Bacillus subtilis...
Post-translational modification of receptor proteins is involved in activation and de-activation signalling systems plants. Both ubiquitination deubiquitination have been implicated plant interactions with pathogens symbionts.Here we present LjPUB13, a PUB-ARMADILLO repeat E3 ligase that specifically ubiquitinates the kinase domain Nod Factor NFR5 has direct role nodule organogenesis events Lotus japonicus. Phenotypic analyses three LORE1 retroelement insertion lines revealed pub13 plants...
Autolysins belong to a protein family involved in peptidoglycan degradation and remodelling. Within this family, NlpC/P60 endopeptidases are the hydrolysis of peptide arm peptidoglycan. In work, putative endopeptidase TTHA0266 from Thermus thermophilus HB8 was overexpressed, purified crystallized. The crystals diffracted 2.4 Å resolution belonged hexagonal space group P6(1), with unit-cell parameters = b 71.19, c 198.68 Å, γ 120°. Selenomethionine-substituted crystallized structure solved by...
SUMMARY Post-translational modification of receptor proteins is involved in activation and de-activation signaling systems plants. Both ubiquitination deubiquitination have been implicated plant interactions with pathogens symbionts. Here we present LjPUB13 , a PUB-ARMADILLO repeat E3 ligase that specifically ubiquitinates the kinase domain Nod Factor NFR5 has direct role nodule organogenesis events Lotus japonicus . Phenotypic analyses three LORE1 retroelement insertion lines revealed pub13...