A5069285867- Plant Stress Responses and Tolerance
- Plant responses to elevated CO2
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Water Relations and Carbon Dynamics
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Plant-Microbe Interactions and Immunity
- Wheat and Barley Genetics and Pathology
- Agriculture, Plant Science, Crop Management
- Genetics and Plant Breeding
- Calcium signaling and nucleotide metabolism
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Ion channel regulation and function
- Tree-ring climate responses
- Fern and Epiphyte Biology
- Bioenergy crop production and management
- Plant Reproductive Biology
- Clostridium difficile and Clostridium perfringens research
- Plant Genetic and Mutation Studies
- Plant and Biological Electrophysiology Studies
- Plant Micronutrient Interactions and Effects
- Polysaccharides and Plant Cell Walls
- DNA and Nucleic Acid Chemistry
University of Tartu
2016-2025
University of Helsinki
2017
National Taiwan University
2017
Estonian University of Life Sciences
2004-2006
Estonian Academy of Sciences
2004
Abscisic acid (ABA) is a key hormone for plant growth, development, and stress adaptation. Perception of ABA through four types receptors has been reported. We show here that impairment perception the PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF RECEPTORS (RCAR) branch reduces vegetative growth seed production leads to severe open stomata ABA-insensitive phenotype, even though other branches remain functional. An Arabidopsis thaliana sextuple mutant impaired in six...
Cysteine-rich receptor-like kinases (CRKs) are transmembrane proteins characterized by the presence of two domains unknown function 26 (DUF26) in their ectodomain. The CRKs form one largest groups protein plants, but biological functions have so far remained largely uncharacterized. We conducted a large-scale phenotyping approach nearly complete crk T-DNA insertion line collection showing that control important aspects plant development and stress adaptation response to biotic abiotic...
Guard cells shrink and close stomatal pores when air humidity decreases (i.e. the difference between vapor pressures of leaf atmosphere [VPD] increases). The role abscisic acid (ABA) in VPD-induced closure has been studied using ABA-related mutants that respond to VPD some studies not others. importance ABA biosynthesis guard versus vasculature for whole-plant regulation is unclear as well. Here, we show Arabidopsis (Arabidopsis thaliana) lines carrying mutations different steps well pea...
Rapid stomatal closure induced by changes in the environment, such as elevation of CO2, reduction air humidity, darkness, and pulses pollutant ozone (O3), involves SLOW ANION CHANNEL1 (SLAC1). SLAC1 is activated OPEN STOMATA1 (OST1) Ca(2+)-dependent protein kinases. OST1 activation controlled through abscisic acid (ABA)-induced inhibition type 2 phosphatases (PP2C) PYRABACTIN RESISTANCE/REGULATORY COMPONENTS OF ABA RECEPTOR (PYR/RCAR) receptor proteins. To address role signaling PYR/RCARs...
Abstract Because abscisic acid (ABA) is recognized as the critical hormonal regulator of plant stress physiology, elucidating its signaling pathway has raised promise for application in agriculture, instance through genetic engineering ABA receptors. PYRABACTIN RESISTANCE1/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF RECEPTORS receptors interact with high affinity and inhibit clade A phosphatases type-2C (PP2Cs) an ABA-dependent manner. We generated allele library composed 10,000 mutant clones...
Land plants are considered monophyletic, descending from a single successful colonization of land by an aquatic algal ancestor. The ability to survive dehydration the point desiccation is key adaptive trait enabling terrestrialization. In extant plants, tolerance depends on action hormone abscisic acid (ABA) that acts through receptor-signal transduction pathway comprising PYRABACTIN RESISTANCE 1-like (PYL)-PROTEIN PHOSPHATASE 2C (PP2C)-SNF1-RELATED PROTEIN KINASE 2 (SnRK2) module....
Summary Plant stomatal responses to single environmental factors are well studied; however, a change in two (or more) – common situation nature have been less frequently addressed. We studied the simultaneous application of opposing six evolutionarily distant mono‐ and dicotyledonous herbs representing different life strategies (ruderals, competitors stress‐tolerators) clarify whether crosstalk between opening‐ closure‐inducing pathways leading response is universal or species‐specific....
Stomatal pore apertures are narrowing globally due to the continuing rise in atmospheric [CO2]. CO2 elevation and plant hormone abscisic acid (ABA) both induce rapid stomatal closure. However, underlying signal transduction mechanisms for CO2/ABA interaction remain unclear. Two models have been considered: (i) enhances ABA concentrations and/or early signaling guard cells closure (ii) merges with at OST1/SnRK2.6 protein kinase activation. Here we use genetics, ABA-reporter imaging,...
Activation of the guard cell S-type anion channel SLAC1 is important for stomatal closure in response to diverse stimuli, including elevated CO2. The majority known activation mechanisms depend on abscisic acid (ABA) signaling. Several lines evidence point a parallel ABA-independent mechanism CO2-induced regulation; however, molecular details this pathway remain scarce. Here, we isolated dominant mutation protein kinase HIGH LEAF TEMPERATURE1 (HT1), an essential regulator CO2 responses,...
Sucrose-non-fermenting-1-related protein kinase-2s (SnRK2s) are critical for plant abiotic stress responses, including abscisic acid (ABA) signaling. Here, we develop a genetically encoded reporter SnRK2 kinase activity. This sensor, named SNACS, shows an increase in the ratio of yellow to cyan fluorescence emission by OST1/SnRK2.6-mediated phosphorylation defined serine residue SNACS. ABA rapidly increases FRET efficiency N. benthamiana leaf cells and Arabidopsis guard cells. Interestingly,...
ABSTRACT Genetic variation between naturally occurring populations provides a unique source to unravel the complex mechanisms of stress tolerance. Here, we have analysed O 3 sensitivity 93 natural Arabidopsis thaliana accessions together with five ‐sensitive mutants acute exposure. The in among was much higher than and corresponding wild types. A subset nine major their responses studied more detail. Among traits assayed, stomatal conductance ( g st ) an important factor determining selected...
Changing atmospheric CO2 levels, climate, and air humidity affect plant gas exchange that is controlled by stomata, small pores on leaves stems formed guard cells. Evolution has shaped the morphology regulatory mechanisms governing stomatal movements to correspond needs of various land groups over past 400 million years. Stomata close in response hormone abscisic acid (ABA), elevated concentration, reduced humidity. Whether active control closure these stimuli are present already mosses,...
Increasing drought and diminishing freshwater supplies have stimulated interest in developing small molecules that can be used to control transpiration. Receptors for the plant hormone abscisic acid (ABA) emerged as key targets this application, because ABA controls apertures of stomata, which turn regulate Here, we describe rational design cyanabactin, an receptor agonist preferentially activates Pyrabactin Resistance 1 (PYR1) with low nanomolar potency. A 1.63 Å X-ray crystallographic...
Significance Relief of repression imposed by negative regulators is a crucial mechanism for plant hormone signaling. Clade A PP2Cs are key ABA signaling that inhibited receptors. Degradation complementary to PYR/PYL/RCAR-mediated ABA-dependent inhibition PP2Cs. We reveal BTB/POZ AND MATH DOMAIN proteins (BPMs), substrate adaptors the multimeric cullin3 (CUL3)-RING-based E3 ligases (CRL3s), target degradation. BPM-dependent degradation required ABA-induced stomatal closure counteract...
Vapour pressure deficit (VPD), the difference between saturation and actual air vapour pressures, indicates level of atmospheric drought evaporative on plants. VPD increases during climate change due to changes in temperature relative humidity. Rising induces stomatal closure counteract VPD-mediated water loss from There are important gaps our understanding molecular VPD-sensing signalling mechanisms guard cells. Here, we discuss recent advances, research directions open questions with...
Significance With the continuing increase in global temperatures, plants transpire more water due to increasing vapor pressure deficit. Stomatal pores close rapidly response rising deficit counteract loss. We demonstrate that mutations stomatal CO 2 signaling pathway do not impair an difference (VPD). Osmotic stress causes cytoplasmic Ca 2+ transients guard cells. Nevertheless, we show diverse investigated higher-order calcium-signaling mutants affect VPD response. reveal B3 family Raf-like...
Strategies to activate abscisic acid (ABA) receptors and boost ABA signaling by small molecules that act as receptor agonists are promising biotechnological tools enhance plant drought tolerance. Protein structures of crop might require modifications improve recognition chemical ligands, which in turn can be optimized structural information. Through structure-based targeted design, we have combined genetic approaches generate an agonist molecule (iSB09) engineer a CsPYL1 receptor, named...
Summary The Arabidopsis guard cell anion channel SLAC 1 is essential for stomatal closure in response to various endogenous and environmental stimuli. Interestingly, here we reveal an unexpected impairment of slac1 alleles on opening. We report that mutations unexpectedly slow opening induced by light, low CO 2 elevated air humidity intact plants this caused the severely reduced activity inward K + (K ) channels cells. Expression transporters involved showed small but significant reductions...
In land plants, stomatal pores on leaf surfaces developed to control gas exchange between and surrounding air, but also enable nutrient uptake cooling. Traits such as density (SD), guard cell size, distribution the upper (adaxial) lower (abaxial) (stomatal ratio), aperture width exhibit notable variation across different genotypes environments. These traits influence photosynthesis, water loss, growth, productivity, pathogen susceptibility. Here, we studied of spring wheat flag leaves their...