A5062176761- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Autophagy in Disease and Therapy
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Plant Stress Responses and Tolerance
- Enzyme Structure and Function
- Plant responses to water stress
- Plant Reproductive Biology
- Horticultural and Viticultural Research
- Lipid metabolism and biosynthesis
- Genetically Modified Organisms Research
- Plant Genetic and Mutation Studies
- Bioeconomy and Sustainability Development
- Plant nutrient uptake and metabolism
- Agricultural risk and resilience
- Wheat and Barley Genetics and Pathology
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Toxoplasma gondii Research Studies
- Genetic Mapping and Diversity in Plants and Animals
- Agriculture Sustainability and Environmental Impact
- Metabolomics and Mass Spectrometry Studies
The University of Queensland
2022-2024
Hebrew University of Jerusalem
2017-2024
Agriculture and Food
2023
ARC Centre of Excellence for Plant Success in Nature and Agriculture
2022-2023
ARC Centre of Excellence in Plant Energy Biology
2020-2022
The University of Western Australia
2020-2022
Australian Research Council
2022
California University of Pennsylvania
2019
Australian National University
2019
Despite world food production keeping pace with population growth because of the Green Revolution, United Nations (UN) State Food Security and Nutrition in World 2022 Report indicates that number people affected by hunger has increased to 828 million 29.3% global insecure, 22% children under five years age stunted. Many more have low-quality, unhealthy diets micronutrient deficiencies leading obesity, diabetes, other diet-related non-communicable diseases. Additionally, current agro-food...
Land plants are considered monophyletic, descending from a single successful colonization of land by an aquatic algal ancestor. The ability to survive dehydration the point desiccation is key adaptive trait enabling terrestrialization. In extant plants, tolerance depends on action hormone abscisic acid (ABA) that acts through receptor-signal transduction pathway comprising PYRABACTIN RESISTANCE 1-like (PYL)-PROTEIN PHOSPHATASE 2C (PP2C)-SNF1-RELATED PROTEIN KINASE 2 (SnRK2) module....
The process of apical dominance by which the bud/shoot tip plant inhibits outgrowth axillary buds located below has been studied for more than a century. Different approaches were used over time, with first physiology era, genetic and then multidisciplinary era. During auxin was thought as master regulator acting indirectly to inhibit bud via unknown secondary messenger(s). Potential candidates cytokinin (CK) abscisic acid (ABA). era screening shoot branching mutants in different species...
Abscisic acid (ABA) is best known for regulating the responses to abiotic stressors. Thus, applications of ABA signaling pathways are considered promising targets securing yield under stress. levels rise in response stress, mounting physiological and metabolic that promote plant survival unfavorable conditions. elicits its effects by binding a family soluble receptors found monomeric dimeric states, differing their affinity co-receptors. However, vivo significance biochemical differences...
Abstract Identification of autophagic protein cargo in plants autophagy-related genes (ATG) mutants is complicated by changes synthesis and degradation. To detect cargo, we measured degradation rate shoots roots Arabidopsis (Arabidopsis thaliana) atg5 atg11 mutants. These data show that less than a quarter proteins changing abundance are probable revealed roles ATG11 ATG5 specific glycolytic enzymes other cytosol, chloroplast, ER-resident proteins, specialized role for from mitochondria...
Somatic embryogenesis (SE) provides alternative methodologies for the propagation of grapevine (Vitis spp.) cultivars, conservation its germplasm resources and crop improvement. In this review, current state knowledge regarding SE as applied to these technologies is presented, with a focus on benefits, challenges, limitations method. The paper comprehensive overview different steps involved in process, including callus induction, maintenance embryogenic cultures, production plantlets....
Rice and wheat provide nearly 40% of human calorie protein requirements. They share a common ancestor belong to the Poaceae (grass) family. Characterizing their genetic homology is crucial for developing new cultivars with enhanced traits. Several genes gene families have been characterized based on rice orthologs. Rice–wheat orthology can identify regions that regulate similar traits in both crops. comparative genomics candidate genomic region identified by association or QTL mapping,...
Abstract Making decisions related to security, whether at the community, national, or regional level, is a highly intricate task. This requires multi‐criteria decision‐making approach that incorporates inputs from various perspectives, including those of science, culture, economics, sustainability, and climate change. In order make successful decisions, it imperative compare alternatives rank consequences relative impacts associated with each option. Such comparisons rankings require careful...
SUMMARY Autophagy is a conserved catabolic process that plays an essential role under nutrient starvation conditions and influences different developmental processes. We observed seedlings of autophagy mutants ( atg2 , atg5 atg7 atg9 ) germinated in the dark showed delayed chloroplast development following illumination. The was characterized by decrease photosynthetic chlorophyll biosynthetic proteins, lower content, reduced size, increased levels proteins involved lipid biosynthesis....
Significance Photoinhibitory high light stress in plants leads to increases markers of protein degradation and transcriptional up-regulation proteases proteolytic machinery, but homeostasis (proteostasis) most enzymes is largely maintained under light, so we know little about the metabolic consequences it beyond photosystem damage. We developed a technique look for rapid turnover events response through 13 C partial labeling detailed peptide mass spectrometry. This analysis reveals...
Autophagy is a catabolic process facilitating the degradation of cytoplasmic proteins and organelles in lysosome- or vacuole-dependent manner plants, animals, fungi. Proteomic studies have demonstrated that autophagy controls shapes proteome has identified both receptor cargo inside autophagosomes. In smaller selection studies, proteomics been used for analysis post-translational modifications target elimination protein–protein interactions between receptors cargo, providing better...
In the pursuit of comprehensive metabolomic profiling, a multiplatform analysis protocol has been developed for Arabidopsis thaliana, utilizing an array analytical techniques to quantify diverse set intracellular metabolites. This encompasses methods extraction and purification metabolites, followed by their using high-performance liquid chromatography (HPLC) amino acids, chromatography-tandem mass spectrometry (LC-MS/MS) central carbon metabolism, gas (GC-MS/MS) open profiling organic...
Today’s crop breeding combined with improved agricultural management has brought substantial increases in food production. But irrigation, fertilizers pest requires a high energy input that creates drain on the already scare fossil fuels. It is thus clear different strategy to be adopted increase productivity further meet needs of rapidly increasing world population. Crop breeders are endeavoring this challenge by developing crops higher yield, better resistance pest, disease and weedicides,...
Abstract Photo-inhibitory high light stress in Arabidopsis leads to increases markers of protein degradation and transcriptional upregulation proteases proteolytic machinery, but proteostasis is largely maintained. We find significant the vivo rate for specific molecular chaperones, nitrate reductase, glyceraldehyde-3 phosphate dehydrogenase, phosphoglycerate kinase other plastid, mitochondrial, peroxisomal, cytosolic enzymes involved redox shuttles. Coupled analysis rates, mRNA levels,...
The regenerated transgenic plants (T0) seeds were sown onto Murashige and Skoog Agar (MSA) plates containing 100µg/ml Carbenicillin antimicrobial agent, BASTA herbicide (15 μg/ml) allowed to grow. Transgenic lines selected by resistant (+). genotype of T1 will be mixtures homozygous (+/+), hemizygous (+/0) (-/-). seedlings grow T2 harvested. Then those grown on MS them, the ratio (+/-) susceptible (-/-) phenotype classes are 3:1. Subsequently, T2- single copy above previously created – line...
A CTAB based method for extracting RNA, which is particularly useful tough tissues. This has been adapted from a protocol that was originally used pine tree tissue, difficult due to the high concentrations of polysaccharides, phenolics, and RNase (Chang, S., Puryear, J. & Cairney, Plant Mol Biol Rep (1993) 11: 113. https://doi.org/10.1007/BF02670468). The described herein an effective alternative TRIzol extractions recovering RNA juvenile de-etiolated
Abstract Identification of autophagic protein cargo in plants by their abundance autophagy related genes ( ATG ) mutants is complicated changes both synthesis and degradation. To detect cargo, we measured degradation rate shoots roots Arabidopsis atg5 atg11 mutant plants. These data show that less than a quarter proteins changing are probable revealed roles ATG11 ATG5 specific cytosol, chloroplast ER-resident proteins, specialized role for from mitochondria chloroplasts. Our support...
Abstract Autophagy is a conserved catabolic process that plays an essential role under nutrient starvation condition and influences different developmental processes. We observed seedlings of autophagy mutants ( atg2 , atg5 atg7, atg9 ) germinated in the dark showed delayed chloroplast development following illumination. The was characterized by decrease photosynthetic chlorophyll biosynthetic proteins, lower content, reduced size, increased levels proteins involved lipid biosynthesis....