A5020678571- Photosynthetic Processes and Mechanisms
- Wheat and Barley Genetics and Pathology
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- Peptidase Inhibition and Analysis
- Chromosomal and Genetic Variations
- Enzyme Structure and Function
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Agriculture, Soil, Plant Science
- Plant Gene Expression Analysis
- Endoplasmic Reticulum Stress and Disease
- Sugarcane Cultivation and Processing
- Plant Molecular Biology Research
The University of Western Australia
2016-2022
Australian Research Council
2022
ARC Centre of Excellence in Plant Energy Biology
2016-2021
Advances in genome sequencing and assembly technologies are generating many high-quality sequences, but assemblies of large, repeat-rich polyploid genomes, such as that bread wheat, remain fragmented incomplete. We have generated a new wheat whole-genome shotgun sequence using combination optimized data types an algorithm designed to deal with large complex genomes. The represents >78% the scaffold N50 88.8 kb has high fidelity input data. Our annotation combines strand-specific Illumina...
We applied 15N labeling approaches to leaves of the Arabidopsis thaliana rosette characterize their protein degradation rate and understand its determinants. The progressive new peptides with measuring decrease in abundance >60,000 existing over time allowed us define 1228 proteins vivo. show that half-lives vary from several hours months based on exponential constant decay for each protein. This was calculated relative isotope peptide fold change during growth. Protein complex membership...
Yield and quality improvement of bread wheat (Triticum aestivum) is a focus in efforts to meet new demands from population growth changing human diets. As the complexity genome unravelled, determining how it used build protein machinery plants key next step explaining detailed aspects development. The specific functions organs during vegetative development role metabolism, degradation remobilisation driving grain production are foundations crop performance have recently become accessible...
Summary Mitochondrial Lon1 loss impairs oxidative phosphorylation complexes and TCA enzymes causes accumulation of specific mitochondrial proteins. Analysis over 400 protein degradation rates using 15 N labelling showed that 205 were significantly different between wild type ( WT ) lon1‐1 . Those proteins included ribosomal proteins, electron transport chain subunits enzymes. For respiratory I V, decreased abundance correlated with higher rate in total extracts. After blue native separation,...
Abiotic stress in plants causes accumulation of reactive oxygen species (ROS) leading to the need for new protein synthesis defend against ROS and replace existing proteins that are damaged by oxidation. Functional plant ribosomes critical these activities, however we know little about impact oxidative on ribosome abundance, turnover, function. Using Arabidopsis cell culture as a model system, induced using 1 µm H2 O2 or 5 menadione more than halve growth rate limit total content. We show...
Advances in genome sequencing and assembly technologies are generating many high quality sequences, but assemblies of large, repeat-rich polyploid genomes, such as that bread wheat, remain fragmented incomplete. We have generated a new wheat whole-genome shotgun sequence using combination optimised data types an algorithm designed to deal with large complex genomes. The represents more than 78% the scaffold N50 88.8kbp has fidelity input data. Our annotation combines strand-specific Illumina...
Significance Photoinhibitory high light stress in plants leads to increases markers of protein degradation and transcriptional up-regulation proteases proteolytic machinery, but homeostasis (proteostasis) most enzymes is largely maintained under light, so we know little about the metabolic consequences it beyond photosystem damage. We developed a technique look for rapid turnover events response through 13 C partial labeling detailed peptide mass spectrometry. This analysis reveals...
Abstract Photo-inhibitory high light stress in Arabidopsis leads to increases markers of protein degradation and transcriptional upregulation proteases proteolytic machinery, but proteostasis is largely maintained. We find significant the vivo rate for specific molecular chaperones, nitrate reductase, glyceraldehyde-3 phosphate dehydrogenase, phosphoglycerate kinase other plastid, mitochondrial, peroxisomal, cytosolic enzymes involved redox shuttles. Coupled analysis rates, mRNA levels,...