A5030372580- Sulfur Compounds in Biology
- Heart Rate Variability and Autonomic Control
- Redox biology and oxidative stress
- Adipose Tissue and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Receptor Mechanisms and Signaling
- Bacterial biofilms and quorum sensing
- Oral microbiology and periodontitis research
- Air Quality and Health Impacts
- Molecular Sensors and Ion Detection
- Folate and B Vitamins Research
- Stress Responses and Cortisol
- Nitric Oxide and Endothelin Effects
- Peroxisome Proliferator-Activated Receptors
- Glutathione Transferases and Polymorphisms
- Sulfur-Based Synthesis Techniques
- Biochemical Analysis and Sensing Techniques
- Regulation of Appetite and Obesity
- Advanced Glycation End Products research
- Metabolism, Diabetes, and Cancer
- Legionella and Acanthamoeba research
- Helicobacter pylori-related gastroenterology studies
- Pancreatic function and diabetes
- Cannabis and Cannabinoid Research
- Olfactory and Sensory Function Studies
Tohoku University
2015-2025
Akita University
2024-2025
Mukogawa Women's University
2020-2025
Kyoto University
2006-2019
Naruto University of Education
2014
University of California, Los Angeles
2014
Yamaguchi University
2006-2013
Kumamoto University
2012-2013
Ajinomoto (Japan)
2009-2012
Ajinomoto (United States)
2008-2012
Significance Reactive sulfur-containing compounds, such as l -cysteine hydropersulfide (CysSSH), reportedly form in mammals. However, the biological relevance of these reactive sulfur species remains unclear. We determined that CysSSH was synthesized from cystine by cystathionine β-synthase and γ-lyase, which turn may contribute to high levels glutathione (>100 μM) other derivatives peptides/proteins formed cells, tissues, plasma mice humans. Compared with hydrogen sulfide, were superior...
Abstract Cysteine hydropersulfide (CysSSH) occurs in abundant quantities various organisms, yet little is known about its biosynthesis and physiological functions. Extensive persulfide formation apparent cysteine-containing proteins Escherichia coli mammalian cells believed to result from post-translational processes involving hydrogen sulfide-related chemistry. Here we demonstrate effective CysSSH synthesis the substrate l -cysteine, a reaction catalyzed by prokaryotic cysteinyl-tRNA...
Endogenous hydrogen polysulfides (H2Sn; n>1) have been recognized as important regulators in sulfur-related redox biology. H2Sn can activate tumor suppressors, ion channels, and transcription factors with higher potency than H2S. Although are drawing increasing attention, their exact mechanisms of action still poorly understood. A major hurdle this field is the lack reliable convenient methods for detection. Herein we report a H2Sn-mediated benzodithiolone formation under mild conditions....
Abstract The mammalian brain is highly vulnerable to oxygen deprivation, yet the mechanism underlying brain’s sensitivity hypoxia incompletely understood. Hypoxia induces accumulation of hydrogen sulfide, a gas that inhibits mitochondrial respiration. Here, we show that, in mice, rats, and naturally hypoxia-tolerant ground squirrels, inversely related levels sulfide:quinone oxidoreductase (SQOR) capacity catabolize sulfide. Silencing SQOR increased hypoxia, whereas neuron-specific expression...
Supersulphides are inorganic and organic sulphides with sulphur catenation diverse physiological functions. Their synthesis is mainly mediated by mitochondrial cysteinyl-tRNA synthetase (CARS2) that functions as a principal cysteine persulphide synthase (CPERS). Here, we identify protective of supersulphides in viral airway infections (influenza COVID-19), aged lungs chronic lung diseases, including obstructive pulmonary disease (COPD), idiopathic fibrosis (IPF). We develop method for breath...
Reactive sulfur species, or persulfides and polysulfides, such as cysteine hydropersulfide glutathione persulfide, are endogenously produced in abundance both prokaryotes eukaryotes, including mammals. Various forms of reactive occur low-molecular-weight protein-bound thiols. The chemical properties great supply these molecular species suggest a pivotal role for persulfides/polysulfides different cellular regulatory processes (e.g., energy metabolism redox signaling). We demonstrated earlier...
Supersulfides, which are defined as sulfur species with catenated atoms, increasingly being investigated in biology. We recently identified pyridoxal phosphate (PLP)-dependent biosynthesis of cysteine persulfide (CysSSH) and related supersulfides by cysteinyl-tRNA synthetase (CARS). Here, we the physiological role CysSSH budding yeast (Saccharomyces cerevisiae) generating a PLP-binding site mutation K109A CRS1 (the ortholog CARS), decreased synthesis also led to reduced chronological aging,...
The physiological importance of reactive sulfur species (RSS) such as cysteine hydropersulfide (CysSSH) has been increasingly recognized in recent years. We have established a metabolomics analysis by using RSS metabolic profiling, which revealed appreciable amounts generated endogenously and ubiquitously both prokaryotic eukaryotic organisms. chemical nature these polysulfides is not fully understood, however, because their or complicated redox-active properties. In our study here, we...
Fluorescent probes are useful tools for the detection of sulfane sulfurs in biological systems. In this work, we report development SSP4, a widely used probe generated our laboratory. We describe its evolution, preparation, and physical/chemical properties. Fluorescence analyses SSP4 determined high selectivity sensitivity to sulfurs, even with interfering presence other species, such as amino acids metal ions. Protocols using relatively quick simple manner persulfidated proteins, including...
Abundant formation of endogenous supersulfides, which include reactive persulfide species and sulfur catenated residues in thiols proteins (supersulfidation), has been observed. We found here that supersulfides catalyze S-nitrosoglutathione (GSNO) metabolism via glutathione-dependent electron transfer from aldehydes by exploiting alcohol dehydrogenase 5 (ADH5). ADH5 is a highly conserved bifunctional enzyme serving as GSNO reductase (GSNOR) down-regulates NO signaling formaldehyde (FDH)...
Thionitrous acid (HSNO), the smallest S-nitrosothiol, is emerging as a potential key intermediate in cellular redox regulation linking two signaling molecules H2 S and NO. However, chemical biology of HSNO remains poorly understood. A major hurdle lack methods for selective detection biological systems. Herein, we report rational design, synthesis, evaluation first fluorescent probe TAP-1 detection. showed high selectivity sensitivity to aqueous media cells, providing useful tool...
Abstract Early detection of esophageal cancer is essential for esophagogastroduodenoscopy and histopathological diagnosis. However, endoscopic examinations are sometimes invasive, which limits their clinical application compliance, traditional blood tumor markers unsuitable screening. The current study aimed to evaluate the usefulness sulfur metabolites as new biomarkers using samples exhaled breath condensate (EBC), can be readily obtained non‐invasive. We collected EBC plasma from 50...
The physiological functions of supersulfides, inorganic and organic sulfides with sulfur catenation, have been extensively studied. Their synthesis is mainly mediated by mitochondrial cysteinyl-tRNA synthetase (CARS2) that as a principal cysteine persulfide synthase. This study aimed to investigate the role supersulfides in joint homeostasis bone regeneration. Using Cars2AINK/+ mutant mice, which KIIK motif CARS2 essential for supersulfide production was replaced AINK, we evaluated fracture...
Tocotrienols, members of the vitamin E family, have been shown to possess anti-inflammatory properties and display activity against a variety chronic diseases, such as cancer, cardiovascular neurological diseases. However, whether tocotrienols contribute prevention inflammatory responses in adipose tissue remains be elucidated. In this study, we examined effects γ-tocotrienol, most common tocotrienol isomer, on tumor necrosis factor-α (TNF-α)-induced by measuring expression adipokines,...
Helicobacter cinaedi is the most common enterohepatic species that causes bacteremia in humans, but its pathogenicity unclear. Here, we investigated possible association of H. with atherosclerosis vivo and vitro. We found infection significantly enhanced hyperlipidaemic mice. Aortic root lesions infected mice showed increased accumulation neutrophils F4/80+ foam cells, which was due, at least partly, to bacteria-mediated expression proinflammatory genes. Although asymptomatic, detection...