A5024321243- Protein purification and stability
- Protein Structure and Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Protein Interaction Studies and Fluorescence Analysis
- Nitric Oxide and Endothelin Effects
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Glycosylation and Glycoproteins Research
- Microfluidic and Capillary Electrophoresis Applications
- Heat shock proteins research
- Sulfur Compounds in Biology
- Analytical Chemistry and Chromatography
- Viral Infectious Diseases and Gene Expression in Insects
- Mass Spectrometry Techniques and Applications
- Study of Mite Species
- Advanced biosensing and bioanalysis techniques
- Dermatological diseases and infestations
- Proteins in Food Systems
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Research and Treatments
Rohto Pharmaceutical (Japan)
2023
Teikyo University
2014-2021
Kumamoto University
1999-2016
RIKEN Center for Computational Science
2013-2015
ID Pharma (Japan)
2001-2005
Osaka University
1998-2001
The plasma membrane of mammalian cells is one the tight barriers against gene transfer by synthetic delivery systems. Various agents have been used to facilitate destabilizing endosomal under acidic conditions, but their utility limited, especially for <i>in vivo</i>. In this article, we report that protein transduction domain human immunodeficiency virus type 1 Tat (Tat peptide) greatly facilitates via destabilization. We constructed recombinant λ phage particles displaying peptide on...
We studied whether i.v. administration of a chemokine after local tumor site irradiation could prevent remaining, as well distant, nonirradiated cell growth by leukocyte recruitment.Tumors were implanted s.c. in the right or both flanks. After at flank, ECI301, human macrophage inflammatory protein-1alpha variant was injected Tumor volumes measured every 3 days treatment.In Colon26 adenocarcinoma-bearing BALB/c mice, repeated daily (over 3-5 consecutive days) 2 mug per mouse ECI301 6 Gy...
IL-1 (interleukin-1) acts as a key mediator of the degeneration articular cartilage in RA (rheumatoid arthritis) and OA (osteoarthritis), where chondrocyte death is observed. It still controversial, however, whether induces death. In present study, viability mouse chondrocyte-like ATDC5 cells was reduced by treatment with IL-1β for 48 h or longer. augmented expression catalytic gp91 subunit NADPH oxidase, gp91phox, well inducible NO synthase cells. Generation nitrated guanosine tyrosine...
Vitrification and slow-freezing methods have been used for the cryopreservation of human pluripotent stem cells (hPSCs). requires considerable skill post-thaw recovery is low. Furthermore, it not suitable large numbers hPSCs. While hPSCs are easy to perform, they usually preceded by a complicated cell dissociation process that yields poor survival. To develop robust method hPSCs, several different cocktails were prepared modifying commercially available freezing medium (CP-1™) containing...
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid. This successfully provided structures for variety macromolecular complexes. paper is focused on the protein bands separated agarose gels. Two methods to PVDF membrane are introduced here, one by contact (diffusion) another electroblotting after pre-treating gels with SDS. The resulted in...
Streptococcus pyogenes thiol proteinase, also known as streptococcal pyrogenic exotoxin B (SpeB), has been suggested to be a major virulence factor in S. infection. SpeB was reported induce apoptosis of host cells, but its mechanism action is not yet fully understood. In this study, we examined the involvement matrix metalloproteinases (MMPs) SpeB-induced apoptosis. We first developed large-scale preparation recombinant and precursors human MMP-9 -2 (proMMPs) by using Escherichia coli...
Nitric oxide (NO) may affect the genomes of various pathogens, and this mutagenesis is particular interest for viral pathogenesis evolution. Here, we investigated effect NO on replication mutation. Exogenous or endogenous had no apparent antiviral influenza A virus Sendai virus. The mutagenic potential was analyzed with fused to a green fluorescent protein (GFP) gene (GFP-SeV). GFP-SeV cultured in SW480 cells transfected vector expressing inducible synthase (iNOS). mutation frequency...
Nitric oxide (NO)-induced nitrative stress of nucleic acids, as evidenced by guanine nitration, appears to be involved in inflammation-induced carcinogenesis. A high incidence lung cancer idiopathic pulmonary fibrosis (IPF) is the major reason for poor prognosis patients with IPF.We immunohistochemically analyzed formation and localization 8-nitroguanine tissues from control subjects, IPF, cancer.Immunohistochemical analysis smoker nonsmoker lungs showed weak immunoreactivity 8-nitroguanine,...
We had developed a H(2)O(2) generating enzyme, polyethylene glycol conjugated D-amino acid oxidase (PEG-DAO), which exhibited potent antitumor activity by toxic reactive oxygen species, namely oxidation therapy, subsequently showed remarkable effect on murine Sarcoma 180 solid tumor, taking advantage of the enhanced permeability and retention effect. Along this line, we report here preparation PEG-DAO use recombinant DAO its using various tumor cell lines models. Recombinant (rDAO) was...