A5069244896- Mesenchymal stem cell research
- Salivary Gland Disorders and Functions
- Bone Metabolism and Diseases
- Osteoarthritis Treatment and Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Oral and Maxillofacial Pathology
- dental development and anomalies
- Cancer-related molecular mechanisms research
- Salivary Gland Tumors Diagnosis and Treatment
- NF-κB Signaling Pathways
- Cancer-related gene regulation
- Proteoglycans and glycosaminoglycans research
- Oral microbiology and periodontitis research
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Oral and gingival health research
- Developmental Biology and Gene Regulation
- Tissue Engineering and Regenerative Medicine
- Immune Response and Inflammation
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Bone and Dental Protein Studies
- Hippo pathway signaling and YAP/TAZ
- Ubiquitin and proteasome pathways
- Lipid metabolism and disorders
Showa University
2015-2024
John Wiley & Sons (United Kingdom)
2020
Hudson Institute
2020
Clock Spring (United States)
2017
Saitama Medical University
2017
Center For Children With Special Needs
2017
Kumamoto University
2014
Thomas Jefferson University
2009-2012
Children's Hospital of Philadelphia
2011
Organoids generated from pluripotent stem cells are used in the development of organ replacement regenerative therapy by recapitulating process organogenesis. These processes strictly regulated morphogen signalling and transcriptional networks. However, precise transcription factors involved organogenesis exocrine glands, including salivary remain unknown. Here, we identify a specific combination two (Sox9 Foxc1) responsible for differentiation mouse embryonic cell-derived oral ectoderm into...
Activation of the Wnt/beta-catenin and retinoid signaling pathways is known to tilt cartilage matrix homeostasis toward catabolism. Here, we investigated possible interactions between these pathways. We found that all-trans-retinoic acid (RA) treatment mouse epiphyseal chondrocytes in culture did increase absence or presence exogenous Wnt3a, as revealed by lymphoid enhancer factor/T-cell factor/beta-catenin reporter activity beta-catenin nuclear accumulation. This stimulation was accompanied...
Summary Interleukin (IL)‐4 and IL‐13 are closely related cytokines known to inhibit osteoclast formation by targeting osteoblasts produce an inhibitor, osteoprotegerin (OPG), as well directly precursors. However, whether their inhibitory actions the same remains unclear. The effect of IL‐4 was stronger than that in osteoclast‐differentiation culture system containing mouse Both induced OPG production similar time‐ dose‐dependent manners. direct inhibition targeted Furthermore,...
IL-1 (interleukin-1) acts as a key mediator of the degeneration articular cartilage in RA (rheumatoid arthritis) and OA (osteoarthritis), where chondrocyte death is observed. It still controversial, however, whether induces death. In present study, viability mouse chondrocyte-like ATDC5 cells was reduced by treatment with IL-1β for 48 h or longer. augmented expression catalytic gp91 subunit NADPH oxidase, gp91phox, well inducible NO synthase cells. Generation nitrated guanosine tyrosine...
In Brief Study Design. Histologic analysis of intervertebral disc (IVD) in three types transgenic mice. Objective. To investigate the role Wnt/β-catenin signaling regulation IVD development and organization. Summary Background Data. -catenin dependent Wnt is one central regulators cartilage during limb skeletal formation. Little known, however, about physiologic relevance this pathway to Methods. Temporal-spatial distribution activity was examined using reporter (TOPGAL) The structural...
Abstract Stem cell-based therapy has been proposed as a promising strategy for regenerating tissues lost through incurable diseases. Side population (SP) cells have identified putative stem in various organs. To examine therapeutic potential of SP hypofunction exocrine glands, isolated from mouse namely, lacrimal and salivary were transplanted into mice with irradiation-induced the respective glands. The secretions both glands recipient restored within 2 months transplantation, although only...
Lymph node metastasis is a major factor for poor prognosis in oral squamous cell carcinoma ( OSCC ). However, the molecular mechanisms of lymph are unclear. We determined that angiopoietin‐like protein 4 ANGPTL 4) m RNA and expression were increased cells established from primary site metastatic cases. In addition, biopsy specimens was correlated with presence metastasis. Therefore, our initial findings suggest expressing may possess ability. Furthermore, culture supernatants metastasized to...
The discovery of circulating tumour DNA molecules created a paradigm shift in biomarkers as predictors recurrence. Non-invasive prenatal testing (NIPT) to detect cell-free foetal maternal plasma is increasingly recognised valuable substitute perceive copy number variation (CNV). This study aimed determine whether the detection samples using NIPT platform could be used prognostic biomarker patients with gynaecological cancer. We conducted prospective containing preoperative from 100 women...
Porphyromonas gingivalis is one of the major pathogens periodontitis, a condition characterized by excessive alveolar bone resorption osteoclasts. The bacterium produces cysteine proteases called gingipains, which are classified according to their cleavage-site specificity into Kgps (lysine-specific gingipains) and Rgps (arginine-specific gingipains). In present study we examined effects gingipains on osteoclast differentiation. co-cultures mouse bone-marrow cells osteoblasts, formation...
Abstract Osteoclasts are terminally differentiated from cells of monocyte/macrophage lineage by stimulation with TNF-related activation-induced cytokine (TRANCE) (receptor activator NF-κB ligand/osteoprotegerin ligand/osteoclast differentiation factor/TNFSF11/CD254). In the present study, we attempted to determine when and how cell fate precursors becomes committed osteoclasts following TRANCE stimulation. Although mouse bone marrow-derived macrophages (BMMs) were able differentiate into...
Dental preparation sometimes causes transient congestion, edema, and necrosis of the pulp. We hypothesized that nitric oxide (NO) is involved in pathophysiological changes pulp after preparation. The mRNA protein expression inducible isoform NO synthase (iNOS) was examined murine dental effects on proliferation, mineralization, apoptosis cells were also studied vitro. found not only iNOS, but mRNAs for alkaline phosphatase plasma membrane glycoprotein-1, expressed NOC-18, an donor,...
Osteoarthritis (OA) is one of most common skeletal disorders and can affect synovial joints such as knee ankle joints. α5 integrin, a major fibronectin receptor, expressed in articular cartilage has been demonstrated to play roles joint development the regulation chondrocyte survival matrix degradation cartilage. We hypothesized that integrin signaling involved pathogenesis OA. To test this, we generated compound mice conditionally ablate using Gdf5Cre system. The were born normally had an...
Interleukin-1β (IL-1β) induces cell death in chondrocytes a nitric oxide (NO)- and reactive oxygen species (ROS)-dependent manner. In this study, increased production of lactate was observed IL-1β-treated mouse chondrocytic ATDC5 cells prior to the onset their death. IL-1β-induced suppressed by introducing an siRNA for monocarboxylate transporter-1 (MCT-1), transporter distributed plasma mitochondrial inner membranes. Mct-1 knockdown also prevented expression phagocyte-type NADPH oxidase...
Osteochondromas and enchondromas are the most common tumors affecting skeleton. can occur as multiple lesions, such those in patients with hereditary exostoses. Unexpectedly, while studying role of β-catenin cartilage development, we found that its conditional deletion induces ectopic chondroma-like formation mice. Postnatal ablation induced lateral outgrowth growth plate within 2 weeks after ablation. The masses were present flanking periosteum by 5 persisted for more than 6 months These...
Hematopoietic pre-B-cell leukemia transcription factor-interacting protein (HPIP) is a corepressor of homeobox (PBX) 1 and known to play role in hematopoiesis. Recently, HPIP was demonstrated promote breast cancer cell proliferation hepatocellular carcinoma growth. Moreover, it has been revealed that PBX proteins, the expression which regulated by HPIP, key roles various organs, including oral squamous (OSCC). Nevertheless, there not any study regarding OSCC. This investigated normal mucosa,...
Dental pulp stem cells (DPSCs) are an attractive cell source for use in cell-based therapy, regenerative medicine, and tissue engineering because DPSCs have a high proliferation ability multidifferentiation capacity. However, several problems associated with the collection preservation of future therapy. In particular, isolation cryopreservation is time consuming expensive. this study, we developed novel method (NCM) dental tissues to isolate suitable after thawing cryopreserved tissue....