A5007374424- Mesenchymal stem cell research
- dental development and anomalies
- Bone and Dental Protein Studies
- Oral and Maxillofacial Pathology
- Bone health and treatments
- Cardiac Imaging and Diagnostics
- Bone Metabolism and Diseases
- Periodontal Regeneration and Treatments
- Coronary Interventions and Diagnostics
- Bone Tissue Engineering Materials
- Laser Applications in Dentistry and Medicine
- Dental materials and restorations
- Photodynamic Therapy Research Studies
- Oral microbiology and periodontitis research
- Tissue Engineering and Regenerative Medicine
- Peripheral Artery Disease Management
- Regulation of Appetite and Obesity
- Circadian rhythm and melatonin
- Endodontics and Root Canal Treatments
- Tumors and Oncological Cases
- Salivary Gland Tumors Diagnosis and Treatment
- Head and Neck Cancer Studies
- Spaceflight effects on biology
- Cerebrovascular and Carotid Artery Diseases
- Pluripotent Stem Cells Research
Tsurumi University
2014-2023
University of Dental Medicine
2019
Southern Tohoku General Hospital
2015
Tokushima University
1995-2010
National Defense Medical College
1986-2003
National Institute of Dental and Craniofacial Research
1997-2000
National Institutes of Health
1997-2000
Cedars-Sinai Medical Center
1991
University of California, Los Angeles
1991
National Medical Research Center of Dentistry and Maxillofacial Surgery
1991
Populations of marrow stromal fibroblasts (MSFs) can differentiate into functional osteoblasts and form bone in vivo. It is not known, however, what proportion MSF precursor cells, colony forming units-fibroblast (CFU-Fs), have osteogenic potential. In the present study, analysis formation vivo by single-colony derived strains human (HMSFs) has been performed for first time. Each strain originated from an individual CFU-F underwent four passages vitro prior to subcutaneous implantation...
We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from blood four mammalian species. These phenotypically resemble but are distinguishable skeletal stem found in bone marrow (stromal cells, “mesenchymal cells”). The blood-borne was proven by an vivo transplantation assay which either polyclonal or single colony–derived strains were transplanted into subcutis immunocompromised mice, donor origin fully differentiated using...
Background. Marrow stromal fibroblasts (MSFs) are known to contain bone precursor cells. However, the osteogenic potential of human MSFs has been poorly characterized. The aim this study was compare capacity mouse and after implantation in vivo. Methods. After vitro expansion, were loaded into a number different vehicles transplanted subcutaneously immunodeficient mice. Results. Mouse within gelatin, polyvinyl sponges, collagen matrices all formed capsule cortical-like surrounding cavity...
Techniques used to repair craniofacial skeletal defects parallel the accepted surgical therapies for bone loss elsewhere in skeleton and include use of autogenous alloplastic materials. Transplantation a marrow stromal cell population that contains osteogenic progenitor cells may be an additional modality generation new bone.Full thickness osseous (5 mm) were prepared cranium immunocompromised mice treated with gelatin sponges containing murine derived from transgenic carrying type I...
Abstract: Melatonin is known to regulate a variety of physiological processes including control circadian rhythms, regulation seasonal reproductive function, body temperature, and so forth. Accumulating evidence from in vitro vivo experiments using rodent chicken has also suggested that melatonin may have an influence on skeletal growth bone formation. However, little about the effects human osteoblasts, which thus remains be elucidated. This study was performed determine whether could...
Introduction Leptin, a 16 kDa anti-obesity hormone, exhibits various physiological properties. Interestingly, skin wound healing was proven to delay in leptin-deficient ob/ob mice. However, little is known on the mechanisms of this phenomenon. In study, we attempted elucidate role leptin skin. Methods Immunohistochemical analysis performed confirm expression receptor (Ob-R) human and mouse Leptin topically administered chemical wounds created back along with sustained-release absorbable...
Five spontaneously transformed cell lines were established from a population of murine bone marrow stromal cells (BMSCs) and the expression profiles phenotype-characteristic genes, patterns in vitro differentiation, osteogenic capacity after vivo transplantation determined for each. All clones expressed stable levels cbfa1, "master" gene, whereas individual phenotypic mRNAs variable within each, suggestive both maturational plasticity vitro. Varying collagen type I alkaline phosphatase (AP)...
Leptin, a 16-kD circulating hormone secreted mainly by white adipose tissue, is product of the obese (ob) gene. Leptin acts on human marrow stromal cells to enhance differentiation into osteoblasts and inhibit adipocytes. also inhibits bone formation through hypothalamic relay. To obtain better understanding potential role leptin in formation, localization endochondral ossification was examined immunohistochemically. High expression identified hypertrophic chondrocytes vicinity capillary...
Bone marrow stromal cells (BMSCs) are a heterogeneous population of derived from colony-forming units-fibroblastic (CFU-Fs). These reside in the bone cavity and capable differentiating into several cell phenotypes including osteoblasts, chondroblasts, hematopoiesis-supporting cells, adipocytes. However, factors that regulate proliferation differentiation BMSC for most part unknown. Since many members receptor tyrosine kinase (RTK) family have been shown to participate growth control various...
Abstract Identification of surface markers involved in osteoblast differentiation provides a method to isolate osteoblasts at various stages maturation. In this study, we examined expression the T lymphocyte antigen, Thy-1, by osteoblastic cells from different species. Murine skeletal progenitor, neonatal calvarial, and adult bone (ABCs) were selected represent distinct Flow cytometric analysis showed that Thy-1 was undetectable on progenitor (mouse limb bud clones 14 17), appeared calvarial...
Introduction Leptin, a 16 kDa circulating anti-obesity hormone, exhibits many physiological properties. Recently, leptin was isolated from saliva; however, its function in the oral cavity is still unclear. In this study, we investigated role of by focusing on effect wound healing mucosa. Methods Immunohistochemical analysis used to examine expression receptor (Ob-R) human/rabbit To investigate mucosa, chemical wounds were created rabbit and topically administered wound. The process repair...
Transplanted osteoprogenitor cells derived from cultured bone marrow stromal (BMSCs) can be used to fabricate pedicled flaps.Prospective, randomized experimental trials.Basic science research laboratory.Immunodeficient female NIH-Bg-Nu-Xid mice, aged 3 months.The BMSCs were harvested the long bones of C57Bl/6 transgenic mice carrying type Ialpha1 collagen-chloramphenicol acetyl transferase reporter gene construct; their numbers expanded in tissue culture. Treated received BMSC...
To apply osteoblasts to bone reconstruction, we proved that transplanted possessed the differentiated osteoblastic function and formed bonelike tissue in vivo after transplantation. First, confirmed dexamethasone (Dex) promoted expression of phenotype human osteoblast culture using reverse-transcription-polymerase chain reaction (RT-PCR). These were cultured for 10 days within collagen sponge, which consists denatured type I collagen, presence or absence 10(-7) M Dex. The along with sponge...
To investigate the presence of side population (SP) cells by Hoechst exclusion method in human adult dental pulp tissue.Human pulp-derived were generated from third molar teeth. The stained with 33342 and sorted into SP or non-SP [main (MP) cells]. Both cell types compared growth RT-PCR analyses.SP that express ABCG2, Nestin, Notch-1 alpha-smooth muscle actin found at frequencies ranging 0.67% to 1.02%. This profile disappeared verapamil. These expressed dentine sialophosphoprotein matrix...
Background and Objective: The effects of laser irradiation on Porphyromonas gingivalis have been reported, but the results are still controversial regarding efficiency because differences light sources conditions. aim this study was to determine wavelength conditions under which most effective inhibitory effect P. growth seen without any photosensitizers. Material Methods: Using an Okazaki large spectrograph, monochromatic spectra ranging from 400 700 nm were evaluated effectively inhibited...