A5032569031- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Bone and Dental Protein Studies
- dental development and anomalies
- High voltage insulation and dielectric phenomena
- Power Transformer Diagnostics and Insulation
- Nuclear Materials and Properties
- Nuclear and radioactivity studies
- Tissue Engineering and Regenerative Medicine
- Nuclear reactor physics and engineering
- Bone Tissue Engineering Materials
- Dental Trauma and Treatments
- Cardiac electrophysiology and arrhythmias
- Dental Implant Techniques and Outcomes
- Oral microbiology and periodontitis research
- Pluripotent Stem Cells Research
- Cleft Lip and Palate Research
- Endodontics and Root Canal Treatments
- Osteoarthritis Treatment and Mechanisms
- Oral and Maxillofacial Pathology
- Adenosine and Purinergic Signaling
- Nerve injury and regeneration
- Ion channel regulation and function
- Thermal Analysis in Power Transmission
- Magnetic Properties and Applications
Aichi Gakuin University
2016-2025
Nihon University
2008-2024
Tokyo University of Science
2009-2023
Chugai Pharma (United States)
2008-2023
Central Research Institute of Electric Power Industry
2020-2023
Shibaura Institute of Technology
2022
The University of Tokyo
2004-2021
Hitachi (Japan)
2021
Mitsubishi Heavy Industries (Germany)
2018-2021
Geospatial Information Authority of Japan
2018-2019
Tooth loss due to periodontal disease, dental caries, trauma, or a variety of genetic disorders continues affect most adults adversely at some time in their lives. A biological tooth substitute that could replace lost teeth would provide vital alternative currently available clinical treatments. To pursue this goal, we dissociated porcine third molar buds into single-cell suspensions and seeded them onto biodegradable polymers. After growing rat hosts for 20 30 weeks, recognizable structures...
Bone augmentation via tissue engineering has generated significant interest. We hypothesized that periosteum-derived cells could be used in place of bone marrow stromal (which are widely used) engineering, but the differences osteogenic potential between these 2 cell types unclear. Here, we compared cells, and investigated optimal osteoinductive conditions for cells. Both were induced, bFGF BMP-2, to differentiate into osteoblasts. Periosteal proliferated faster than markers indicated more...
Abstract Epithelial cell rests of Malassez (ERM) are quiescent epithelial remnants Hertwig's root sheath (HERS) that involved in the formation tooth roots. After completion crown formation, HERS converted from cervical loop cells, which have potential to generate enamel for formation. Cervical cells differentiate into ameloblasts. Generally, no new ameloblasts can be generated HERS, however this study demonstrated subcultured ERM ameloblast‐like and enamel‐like tissues combination with...
In vivo models have been required to demonstrate relative cardiac safety, but model sensitivity has not systematically investigated. Cross-species and human translation of repolarization delay, assessed as QT/QTc prolongation, compared employing common methodologies across multiple species sites. Therefore, the accurate results within between preclinical species, man, remains problematic. Six pharmaceutical companies entered into an informal consortium designed collect high-resolution...
The successful regeneration of complex tooth structures based on tissue-engineering principles was recently reported. process this regeneration, however, remains poorly characterized. In study, we have used histochemistry to examine the tissue engineered teeth in order determine cell types that give rise these structures. Porcine third molar buds were dissociated into single-cell suspensions and seeded onto a biodegradable polyglycolic acid polymer scaffold. Following varying periods growth...
In this paper, the streaming electrification in core type forced oil cooled power transformers is investigated, using a full-scale model transformer and various experimental models. It has been clarified that influenced mainly by flow rate, temperature, surface conditions of insulating materials characteristics oil. Electrostatic discharge occurs eventually when amount local charge exceeds certain limit.
We describe a novel role for CD271 in the differentiation of mesenchymal stem cells (MSCs), including deciduous dental pulp (DDPSCs) and murine multipotent MSCs (C3H10T1/2 cells). The CD271(+) subpopulation (CD271(+)/DDPSCs) forced expression C3H10T1/2 (10T271) were analyzed by fluorescence-activated cell sorting. was detected DDPSCs that expressed both CD44 CD90, simultaneously, clonogenic capacity CD271(+)/DDPSCs higher than CD271(-)/DDPSCs CD90. Further, into osteoblasts adipocytes...
Alkaline phosphatase (ALP) is generally believed to be a faithful marker of osteoblast differentiation, and its expression induced by bone morphogenetic protein-2 (BMP-2) dexamethasone (Dex). However, the effects combined administration BMP-2 Dex on ALP transcription have not been extensively examined. In this study, we found that synergistically increase levels in mouse C3H10T1/2 pluripotent stem cells. switching from one inducer other, adding or cell cultures at different times, was no...
Lipid-free fibroblast-like cells, known as dedifferentiated fat (DFAT) can be generated from mature adipocytes with a large single lipid droplet. DFAT cells re-establish their active proliferation ability and transdifferentiate into various cell types under appropriate culture conditions. The first objective of this study was to compare the multilineage differentiation potential that adipose-derived stem (ASCs) on mesenchymal cells. We obtained ASCs inbred rats found rat possess higher...
Abstract Background Extracellular vesicles (EVs) are known to be secreted by various cells. In particular, mesenchymal stem cell (MSC)-derived EVs (MSC-EVs) have tissue repair capacity and anti-inflammatory properties. Dental pulp cells (DPSCs), which MSCs isolated from tissue, less invasive the body than other can collected young individuals. this study, we investigated efficacy of DPSCs (DPSC-EVs) for bone formation. Methods DPSC-EVs were culture medium DPSCs. unilaterally injected along...
Abstract Myostatin, a member of the transforming growth factor-β superfamily, is an attractive target for muscle disease therapy because its role as negative regulator and strength. Here, we describe novel antibody therapeutic approach that maximizes potential myostatin-targeted therapy. We generated antibody, GYM329, specifically binds latent form myostatin inhibits activation. Additionally, via “sweeping technology”, GYM329 reduces or “sweeps” in plasma. Compared with conventional...
Osseointegration is essential for successful implant treatment. However, the underlying molecular mechanisms remain unclear. In this study, we focused on decorin (DCN), which was hypothesized to be present in proteoglycan (PG) layer at interface between bone and titanium oxide (TiOx) surface. We utilized DCN RNA interference human marrow mesenchymal stem cells (hBMSCs) investigate its effects PG formation, proliferation, initial adhesion, cell extension, osteogenic capacity, fibrotic...
Osteoblasts and adipocytes originate from common mesenchymal progenitor cells although a number of compounds can induce osteoblastic adipogenic differentiation cells, the underlying mechanisms have not been elucidated. The present study examined synergistic effects dexamethasone (Dex) bone morphogenetic protein (BMP)-2 on clonal isolated rat calvaria into osteoblasts adipocytes, as well timing treatment. Cells were cultured for various periods time in presence Dex and/or BMP-2. When treated...
Recently, the possibility of tooth tissue engineering has been reported. Although there are a number available materials, information about scaffolds for is still limited. To improve manageability engineering, effect on in vivo regeneration was evaluated. Collagen and fibrin were selected this study based biocompatibility to dental papilla-derived cells results compared with those polyglycolic acid (PGA) fiber beta-tricalcium phosphate (beta-TCP) porous block, which commonly used tooth,...
This study examined the effect of extracellular matrix (ECM) on osteogenic differentiation capacity and osteogenesis dental follicle cells. Single cell-derived porcine cells (DFC-I) obtained at early stage crown formation in tooth were subcultured characterized using periodontal ligament (PDLC) bone marrow-derived mesenchymal stem (BMSC) as comparison cell populations. The ECM constituents including collagen type I, fibronectin, laminin, IV DFC-1 into osteogenic-lineage was evaluated vitro....