A5055315642- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Bone and Dental Protein Studies
- Nerve injury and regeneration
- Cleft Lip and Palate Research
- Tendon Structure and Treatment
- Fibroblast Growth Factor Research
- Foot and Ankle Surgery
- Oral microbiology and periodontitis research
- Electrospun Nanofibers in Biomedical Applications
- Proteoglycans and glycosaminoglycans research
- Dental Trauma and Treatments
- Orthopedic Surgery and Rehabilitation
- Diabetes Treatment and Management
- Lower Extremity Biomechanics and Pathologies
- Oral and Maxillofacial Pathology
- Cellular Mechanics and Interactions
- Connective tissue disorders research
- Animal Genetics and Reproduction
- dental development and anomalies
- Developmental Biology and Gene Regulation
- Streptococcal Infections and Treatments
- Collagen: Extraction and Characterization
Nippon Dental University
2023-2025
Nihon University
2012-2024
Nagoya University
2024
Kyoto University
2024
Honda (Japan)
2024
Kyushu Nutrition Welfare University
2022-2023
Aichi Gakuin University
2018-2022
Surugadai Nihon University Hospital
2013-2014
Jikei University School of Medicine
1969
Lipid-free fibroblast-like cells, known as dedifferentiated fat (DFAT) can be generated from mature adipocytes with a large single lipid droplet. DFAT cells re-establish their active proliferation ability and transdifferentiate into various cell types under appropriate culture conditions. The first objective of this study was to compare the multilineage differentiation potential that adipose-derived stem (ASCs) on mesenchymal cells. We obtained ASCs inbred rats found rat possess higher...
Abstract Non-alcoholic steatohepatitis (NASH), caused by fat buildup, can lead to liver inflammation and damage. Elucidation of the spatial distribution fibrotic tissue in fatty NASH be immensely useful understand its pathogenesis. Thus, we developed a novel serial section-3D (SS3D) technique that combines high-resolution image acquisition with 3D construction software, which enabled highly detailed analysis mouse extraction quantification stained tissues. Moreover, studied underexplored...
Regeneration of damaged periodontium is challenging due to its multi-tissue composition. Mesenchymalstem cell-based approaches using adipose-derived stromal cells (ASCs) may contribute toperiodontal reconstruction, particularly when combined with the use scaffolds maintain aspace for new tissue growth. The aim this study was assess regenerative potential ofASCs derived from inbred or outbred rats in combination novel solid composed ofPLGA (Poly d,l-lactic-co-glycolic acid) (PLGA-scaffolds)....
Inorganic polyphosphate (PolyP), a polymer of orthophosphate, strongly promotes mineralized tissue formation. This study explored the conditions necessary for PolyP to induce calcified deposits in cartilage and assessed role insulin modulating PolyP-induced calcification. Murine chondrogenic ATDC5 cells were cultured under growth, mineralization, or calcification conditions, with without insulin. Calcified nodules stained Alizarin Red S, conditioned media analyzed pH lactate concentration...
Bone marrow-derived mesenchymal stem cells (BMMSCs) remain the most widely used source of osteogenic in bone tissue engineering research. A cell-based treatment for alveolar ridge augmentation has received attention as an alternative to grafting. In present study, BMMSC transplantation into tooth extraction sockets C57BL/6J mice was evaluated regeneration. The first right maxillary molars were extracted, and then BMMSCs (PDGFRα+ Sca-1+ CD45− TER119− cells) isolated from femoral tibial marrow...
We previously generated induced pluripotent stem (iPS) cells from human dental pulp of deciduous teeth. Neural crest (NCCs) play a vital role in the development oral and maxillofacial region. Therefore, NCCs represent cell source for bone, cartilage, tooth-related tissue engineering. In this study, we examined whether iPS are capable differentiating into through modification embryonic protocol. First, were dissociated single then reaggregated low-cell-adhesion plates with neural induction...
Tissue engineering is a promising approach to supplement existing treatment strategies for craniofacial bone regeneration. In this study, type I collagen scaffold made from recombinant peptide (RCP) with an Arg-Gly-Asp motif was developed, and its effect on regeneration in critical-size mandibular defects evaluated. Additionally, the combined of lipid-free dedifferentiated fat (DFAT) cells assessed. Briefly, DFAT were separated mature adipocytes by using ceiling culture technique based...
Adipose-derived stem cells (ASCs) secrete various growth factors to promote wound healing and regenerate tissues, such as bone, cartilage, fat tissue. Subcutaneous adipose tissue is a considerable cell source in clinical practice can be collected relatively easily safely under local anesthesia. Moreover, platelet-rich plasma (PRP), component containing many platelets purified by centrifuging the blood, also promotes healing. PRP gelled therefore attracting attention scaffolding material for...
Dedifferentiated fat (DFAT) cells derived from mature adipocytes have mesenchymal stem cells' (MSCs) characteristics. Generally, are 60–110 μm in diameter; however, association between adipocyte size and dedifferentiation efficiency is still unknown. This study, therefore, investigated the of based on cell diameter. Buccal pad was harvested five human donors dissociated by collagenase digestion. After exclusion unwanted stromal centrifugation, floating were collected their distribution...
The existence of progenitor/mesenchymal stem cells (MSCs) was demonstrated previously in human primary/deciduous teeth. In this study, we examined dental pulp from root portion (root cells) primary teeth without discernible resorption and compared them with the crown (crown cells). Root were characterized to each other based on progenitor/MSC characteristics their generation efficiency induced pluripotent (iPS) cells. included manifesting typical properties such as osteogenic adipogenic...
In the present study, we examined effect of oriented collagen tube (OCT) implantation on recovery sensory function resected rat sciatic nerve.After a 10-mm long portion nerve was resected, an OCT placed in site defect. Recovery evaluated using Von Frey test every 3 days after surgery. The regenerated tissue were histologically and ultrastructurally analyzed 2 4 weeks surgery.The reflexes group restored to level that intact 15 days. Hematoxylin eosin staining revealed cross-linking between...
Purpose: Bacterial cells in mature dental plaque produce a high concentration of short-chain fatty acids (SCFAs) such as butyrate and propionate. SCFA-treatment on human gingival epithelial Ca9-22 induced cell death. However, the exact mechanism underlying death remains unclear. In this study, relationship between reactive oxygen species (ROS) autophagy induction during SCFA-induced was examined.
Periodontitis is one of the diabetic complications due to its high morbidity and severity in patients with diabetes. The prevention periodontitis especially important because relationship between diabetes bidirectional. Here, we evaluated impacts glucagon-like peptide-1 (GLP-1) receptor agonist liraglutide on amelioration periodontitis. Five-wk-old Male Sprague–Dawley (SD) rats ( <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mi>n</a:mi> <a:mo>=</a:mo> <a:mn>30</a:mn>...
The transplantation of dedifferentiated fat (DFAT) cells in combination with poly(d,l-lactic-co-glycolic acid) (PLGA) scaffolds has previously been proven as an effective approach promoting periodontal tissue regeneration a rat fenestration defect model. aim this study was to assess the regenerative potential DFAT model three-wall bone defect. Three-wall defects were created bilaterally on mesial side maxillary first molars and either left untreated or treated by implantation PLGA alone....
Objective Mesiodentes are usually found in the central position of upper or lower jaw as supernumerary teeth. Here, we obtained 10 mesiodentes and three permanent teeth ( PT ) separated dental pulp DP from these into crown root portions. We then characterized compared isolated portion‐derived cells (crown cells) with (root using a range vitro assays. Materials methods Crown were examined for cell surface marker expression, colony‐forming unit‐fibroblast CFU ‐F), proliferation, cycle...
The association between diabetes and periodontal disease is considered to be bidirectional. However, there still controversy surrounding the relationship type 1 diabetes. We investigated whether insulin improves periodontitis without any local treatments for under conditions using ligature-induced experimental model.Type diabetic rats were induced by streptozotocin injection. Experimental was ligature in normal rats. Half of treated with insulin. Two weeks after ligature, evaluated.Insulin...
This study aimed to examine the bone-forming ability of medium-cross-linked recombinant collagen peptide (mRCP) particles developedbased on human type I, contains an arginyl-glycyl-aspartic acid-rich motif, fabricated as bone filling material, compared that autologous graft. Calvarial defects were created in immunodeficient rats though a surgical procedure. The divided into 2 groups: mRCP graft and tibia (bone graft). formation potential was evaluated by micro-computed tomography...
Bone marrow-derived multipotent stromal cells (BMSCs) have potent antiinflammatory effects. This study aimed to investigate the potential of BMSCs using a mouse model ligature-induced periodontitis. were isolated from femurs and tibiae mice. Periodontitis was induced by placing ligature around right maxillary second molar. After 3 days, mice administered BMSC in gingiva mesial interdental papilla ligatured The non-ligatured that not served as controls. Differences inflammatory infiltration...
The effects of transplanted human dental pulp-derived cells (DPCs) on peripheral nerve regeneration were studied in a rat model sciatic crush injury. In one group, DPCs into the compression site (cell transplantation group); control group underwent no (crushed group). Sciatic was determined based recovery motor function and histological immunohistochemical analyses. cell showed improved compared with crushed using CatWalk XT system, which corresponded to higher ratio tibialis anterior muscle...
Two types of dentition are generated in a human's lifetime: the primary dentition, followed by permanent dentition. Undoubtedly, teeth essential for speech and mastication both dentitions, but it is becoming apparent that dental pulp also plays role harboring mesenchymal stem cells (MSCs). To date, three kinds MSCs derived from have been established: tooth, immature apical papilla. The considered particularly good source MSCs; can be obtained extracted teeth, which humans 20. past decade has...
A novel animal experimental model of congenital bone defects without surgical intervention is needed to better mimic human alveolar cleft. We aimed establish a new cleft using rat palatine fissures that are present in the central portion maxilla. The on left and right sides separated by nasal septum. In this study, we examined potential repair an autologous implant beta-tricalcium phosphate (β-TCP) fissure as model. results implants were evaluated micro-computed tomography histological...