A5057605690- Oral microbiology and periodontitis research
- Bone Metabolism and Diseases
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Bone health and treatments
- Antimicrobial Peptides and Activities
- Endodontics and Root Canal Treatments
- Cell Adhesion Molecules Research
- Inflammatory mediators and NSAID effects
- Salivary Gland Disorders and Functions
- Oral Health Pathology and Treatment
- Bone and Dental Protein Studies
- T-cell and B-cell Immunology
- Oral and gingival health research
- Dental Radiography and Imaging
- Immunotherapy and Immune Responses
- Periodontal Regeneration and Treatments
- HIV/AIDS oral health manifestations
- Blood disorders and treatments
- Dental Implant Techniques and Outcomes
- Macrophage Migration Inhibitory Factor
- Monoclonal and Polyclonal Antibodies Research
- Neuropeptides and Animal Physiology
- Cleft Lip and Palate Research
- Erythrocyte Function and Pathophysiology
Nova Southeastern University
2017-2025
South University
2018-2025
Southeastern University
2022-2023
Harvard University
2004-2018
University Hospital Bonn
2015
Tufts University
2005
Ehime University
2003
Hiroshima University
2003
Harvard University Press
2003
Novant Health Forsyth Medical Center
1999
Periodontal disease infection with oral biofilm microorganisms initiates host immune response and signs of periodontitis, including bone resorption. This review delineates some mechanisms underlying the in periodontal alveolar Activated T lymphocytes have been historically implicated experimental An rat adoptive transfer/gingival challenge model has demonstrated to require antigen-specific gingival instillation antigen LPS for Interference costimulatory interactions between cells...
ABSTRACT The antimicrobial peptides human β-defensin-1 (hBD1), hBD2, hBD3, and CAP18 expressed by keratinocytes have been implicated in mediation of the innate defense against bacterial infection. To gain insight into Staphylococcus aureus infection, susceptibility S. , including methicillin-resistant (MRSA), to these was examined. Based on quantitative PCR, expression hBD2 mRNA significantly induced contact with hBD3 slightly induced, while hBD1 constitutively irrespective presence . Ten...
Toll-like receptors (TLRs) play a key role in the innate immune responses to periodontal pathogens disease. The present study was performed determine roles of TLR2 and TLR4 signaling alveolar bone resorption, using Porphyromonas gingivalis-associated ligature-induced periodontitis model mice. Wild-type (WT), Tlr2(-/-), Tlr4(-/-) mice (8 10 weeks old) C57/BL6 background were used. Silk ligatures applied maxillary second molars presence or absence live P. gingivalis infection. Ligatures...
Periodontal disease is an infection in which destruction occurs at sites remote from the infection, resulting pathological pocketing. Intervening between and a dense mononuclear inflammatory infiltrate. It has been suggested that this infiltrate might have characteristics destructive potential of Th1-type T lymphocytes. To ascertain nature infiltrates we investigated expression mRNA for IL-2, IL-5, IFN-γ by gingival cells (GMC) healthy (n = 8) or adult periodontitis (AP) patients 25) using...
Abstract The CD28 costimulation at TCR signaling plays a pivotal role in the regulation of T cell response. To elucidate cells periodontal disease, system transfer with TCR/CD28-dependent Th1 or Th2 clones was developed rats. Gingival injection specific Ag, Actinobacillus actinomycetemcomitans 29-kDa outer membrane protein, and LPS could induce local bone resorption 10 days after Ag-specific clone cells, but not cells. Interestingly, presence required only for induction also IgG2a...
Objective: Interleukin‐10 is an anti‐inflammatory cytokine that reduces periapical bone loss, but its role in periodontal loss unclear. In the present study, we tested hypothesis endogenous interleukin‐10 a potent suppressor of Porphyromonas gingivalis ‐induced alveolar vivo . Methods: knockout (–/–) and wild‐type mice were inoculated intraorally with P. Non‐infected animals served as negative controls. Alveolar gingival levels, gene expression assessed using morphometric analysis,...
Abstract Host immune responses play a key role in periodontal diseases. We have found that B lymphocytes human lesions bear abundant receptor activator of NF-κB ligand (RANKL), major factor the regulation osteoclast differentiation. The purpose this study was to evaluate Actinobacillus actinomycetemcomitans-responsive their level RANKL expression and effects on bone resorption. Congenitally athymic Rowett rats received injections formalin-fixed A. actinomycetemcomitans into gingival...
Accumulated lines of evidence suggest that hyperimmune responses to periodontal bacteria result in the destruction connective tissue and alveolar bone. The etiological roles onset progression disease (PD) are well documented. However, mechanism underlying engagement RANKL-mediated bone resorption remains unclear. Therefore, this review article addresses three critical subjects. First, we discuss earlier studies immune intervention, ultimately leading identification bacteria-reactive...
Summary Anti-microbial peptides produced from mucosal epithelium appear to play pivotal roles in the host innate immune defence system oral cavity. In particular, human beta-defensins (hBDs) and cathelicidin-type anti-microbial peptide, LL-37, were reported kill periodontal disease-associated bacteria. contrast well-studied hBDs, little is known about expression profiles of LL-37 gingival tissue. this study, expressed tissue analysed using immunohistochemistry enxyme-linked immunosorbent...
No consensus has yet been reached to associate oral bacteria conclusively with the etio-pathogenesis of bisphosphonate-induced osteonecrosis jaw (BONJ). Therefore, present study examined effects on development BONJ-like lesions in a mouse model. In pamidronate (Pam)-treated mice, but not control non-drug-treated tooth extraction followed by infection Fusobacterium nucleatum caused and delayed epithelial healing, both which were completely suppressed broad-spectrum antibiotic cocktail....
In response to a defined panel of stimuli, immature macrophages can be classified into two major phenotypes: proinflammatory (M1) and anti-inflammatory (M2). Although both phenotypes have been implicated in several chronic inflammatory diseases, their direct role bone resorption remains unclear. The present study investigated the possible effects M1 M2 on RANKL-induced osteoclastogenesis. osteoclastogenesis assays using RAW264.7 cells or marrow as osteoclast precursors, addition...
Sjögren's syndrome (SjS) is a chronic autoimmune disease that mainly targets the salivary and lacrimal glands. It has been controversial whether anti-muscarinic type 3 receptor (α-M3R) autoantibodies in patients with SjS inhibit intracellular trafficking of aquaporin-5 (AQP5), water transport protein, leading to secretory dysfunction. To address this issue, GFP-tagged human AQP5 was overexpressed gland cells (HSG-hAQP5) monitored plasma membrane following carbachol (CCh, M3R agonist)...
Prior studies have demonstrated an increased frequency of antibodies to Porphyromonas gingivalis (Pg), a leading agent periodontal disease, in rheumatoid arthritis (RA) patients. However, these patients generally had long-standing and clinical associations with were inconsistent. Our goal was examine Pg antibody responses their early RA prior after disease-modifying antirheumatic drug (DMARD) therapy. Serum samples from 50 DMARD-naïve tested using enzyme-linked immunosorbent assay whole-Pg...
Porphyromonas gingivalis (Pg) infection causes periodontal disease and exacerbates rheumatoid arthritis (RA). It is reported that inoculation of periodontopathogenic bacteria (i.e., Pg) can alter gut microbiota composition in the animal models. Gut dysbiosis human has shown strong associations with systemic diseases, including RA, diabetes mellitus, inflammatory bowel disease. Therefore, this study investigated dysbiosis-mediated by Pg oral an experimental model mouse.Pg cavity twice a week...
Diabetic retinopathy (DR) is one of the leading causes blindness. Periodontitis highest oral incidences and has been closely related to various systemic conditions through Porphyromonas gingivalis ( P. ). OMVs, derived from , can cause endothelial dysfunction potentially affect microvascular diseases. Current epidemiological studies provide limited evidence suggesting that periodontitis associated with DR. However, there a lack basic research elucidating how affects severity This study aimed...
Actinobacillus actinomycetemcomitans (Aa) is one of the pathogenic bacteria involved in periodontal diseases. We have previously identified six major outer membrane proteins (Omps) Aa Y4. Among them an Omp with high molecular mass, designated Omp100, which has homology to a variety virulence factors. Electron microscopic observation indicated that Omp100 randomly localized on cell surface Aa. Y4 been shown adhere and invade KB or normal human gingival keratinocytes. Anti-Omp100 antibody...
Abstract Differentiated CD4 T cells can be divided into Th1 and Th2 types based on the cytokines they produce. Differential expression of chemokine receptors either Th1-type or Th2-type cell suggests that differ not only in cytokine production but also their migratory capacity. Stimulation endothelial with IFN-γ selectively enhanced transmigration cells, a transendothelial migration assay. Enhanced was dependent RANTES produced by as indicated findings Ab neutralizing RANTES, to its receptor...
The effects of the potassium channel (Kv1.3) blocker kaliotoxin on T-cell-mediated periodontal bone resorption were examined in rats. Systemic administration abrogated conjunction with decreased RANKL mRNA expression by T-cells gingival tissue. This study suggests a plausible therapeutic approach for inflammatory targeting Kv1.3.Kv1.3 is critical to counterbalance calcium influx at T-cell receptor activation. It not known if Kv1.3 also regulates antigen-activated T-cells, and consequently...