A5046953707- Wound Healing and Treatments
- Cancer-related Molecular Pathways
- Intracranial Aneurysms: Treatment and Complications
- Skin and Cellular Biology Research
- Neurosurgical Procedures and Complications
- Cancer Research and Treatments
- Corneal Surgery and Treatments
- Pharmacy and Medical Practices
- Wnt/β-catenin signaling in development and cancer
- Hair Growth and Disorders
- Pluripotent Stem Cells Research
- Nuclear Materials and Properties
- T-cell and B-cell Immunology
- Spine and Intervertebral Disc Pathology
- Traumatic Brain Injury and Neurovascular Disturbances
- Vascular Malformations Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Cervical and Thoracic Myelopathy
- Veterinary Equine Medical Research
- Cerebrospinal fluid and hydrocephalus
- Immune Cell Function and Interaction
- Spinal Fractures and Fixation Techniques
- Cerebrovascular and Carotid Artery Diseases
- Cancer Cells and Metastasis
Japan Lifeline (Japan)
2025
Ohkawara Neurosurgical Hospital
2011-2024
Boston University
2017-2023
Nakamura Memorial Hospital
1991-2021
Institute of Molecular and Cell Biology
2017
In-Q-Tel
2017
University of Pennsylvania
2010-2015
California Institute for Regenerative Medicine
2014
Hitachi (Japan)
1983-2010
Dentsply Sirona (United States)
2010
Tumor suppressor p53 has been shown to transactivate epidermal growth factor receptor (EGFR) expression through binding a putative responsive element in the EGFR promoter between nucleotides −265 and −239 (EGFRp53RE). Isotypes of p63 gene products, recently identified as relatives, have similar function several target promoters. However, our results indicate that TAp63γ very low ability bind EGFRp53RE surprisingly represses both basal activity endogenous expression. Transient transfection...
Summary Reasons for performing study: Abnormal epidermal stem cell regulation may contribute to the pathogenesis of equine chronic laminitis. Objective: To analyse involvement p63, a regulator proliferative potential, in Methods: Epidermal tissues from skin, coronet and lamellae dorsal foot were harvested 5 horses with laminitis control horses. Tissues analysed using histopathology, immunofluorescence microscopy quantitative immunoblotting Results: Hoof laminitic had lower frequency p63...
We have recently identified a second p53 -related p73L gene, also referred to as p63 / p51 p40 KET which encodes the 2 major isoforms and resulting from different exon usage at their amino terminal regions. Although are suspected play oncogenic tumour suppressive roles in mammalian cells, respectively, no evidence of linkage between expression these human cancers has been reported so far. In this study, we first investigated profile various cell lines found that novel isoform, termed...
Genetic mutation of p53, which monitors DNA damage and operates cellular checkpoints, is a major factor in the development human malignancies. A novel gene p63/p73L/p51, encoding protein with significant homology to p53 p73, was recently identified at 3q27-9. To investigate penetration p63 cervical carcinogenesis, transcription analyses were performed carcinoma. certain isotype called TAp63gamma encodes acidic N-terminus possesses short C-terminus. Using reverse transcriptase-polymerase...
During the thymic development of alphabeta lineage T cells, maturation CD4- CD8- double-negative (DN) cells into CD4+ CD8+ double-positive is accompanied by induction TCR beta allelic exclusion. Recent studies have shown that these events are regulated signals through pre-TCR complex which consists beta, alpha and CD3 components. The Vbeta germline transcripts detected prior to chain gene rearrangements in DN thymocytes. To examine effects pre-TCR-mediated on transcription, we analyzed...
Although Xenopus laevis has been a model of choice for comparative and developmental studies the immune system, little is known about organogenesis thymus, primary lymphoid organ in vertebrates. Here we examined expression three transcription factors that have functionally associated with pharyngeal gland development, gcm2, hoxa3, foxn1, evaluated neural crest contribution to thymus development.
Mouse models have been used to provide primary cells study physiology and pathogenesis of epithelia. However, highly efficient simple approaches propagate mouse epithelial remain challenging. Here, we show that pharmacological inhibition TGF-β signaling enables long-term expansion p63+ progenitor in low Ca2+ media without the need cell-purification steps or support by a feeder cell layer. We find is operative keratinocytes conventional cultures as determined nuclear Smad2/3 localization....
The transcription factor p63 (Trp63) plays a key role in homeostasis and regeneration of the skin. gene is transcribed from dual promoters, generating TAp63 isoforms with growth suppressive functions dominant-negative ΔNp63 opposing properties. also encodes multiple carboxy (C)-terminal variants. Although mutations C-terminal variants have been linked to pathogenesis p63-associated ectodermal disorders, physiological C-terminus poorly understood. We report here that deletion mice leads...
Background The limited characterization of equine skin, eye and hoof epithelial stem cell ( ESC ) differentiation markers impedes the investigation physiology pathophysiology these tissues. Hypothesis/Objectives To characterize marker expression in tissues eye, haired skin capsule. Methods Indirect immunofluorescence microscopy immunoblotting were used to detect tissue localization keratin (K) isoforms K3, K10, K14 K124, transcription factor p63 (a s) phosphorylated [pp63; a transition...
Seventy percent of the murine TCRbeta locus (475 kb) was deleted to generate a large (beta(LD)) allele investigate possible linkage between germline transcription, recombination frequency, and allelic exclusion TCR Vbeta genes. In these beta(LD/LD) mice, gene contained only four genes at 5' side locus, consequently, Vbeta10 located in original Dbeta1-Jbeta1cluster within Ebeta regulatory region. We showed that frequency expression are strongly biased mutant mice even though proximity other...
This paper describes the influence of rotor eccentricity on permanent magnet synchronous motor characteristics by a magnetic field analysis and several experiments. The unbalances force thus increases mechanical loss. Furthermore, circulating current generated in two three-phase windings connected parallel results an increase electric power loss motor. Therefore, we presented that flux barrier is effective to reduce harmful effect eccentricity.
Abstract The surface of the middle ear is composed tympanic membrane (TM) and mucosa (MEM). A number diseases conditions such as otitis media, cholesteatoma, perforation TM have been reported to cause dysfunction ear, ultimately leading high-frequency hearing loss. Despite its importance in repairing damaged tissues, stem/progenitor cells MEM epithelia remains largely uncharacterized due, part, lack an optimal methodology expand maintain long-term. Here, we show that suppression TGF-β...
We have generated mutant mice in which TCR beta chain enhancer (E(beta)) was replaced with the alpha (E(alpha)). Using this mouse model, we analyzed (i) recombination status of genes after functional V(D)J rearrangements occurred first allele during double-negative (DN)-to-double-positive (DP) transition and (ii) involvement E(beta) for expression rearranged genes. Our data show that E(alpha) substituted function to express a similar extent chains exactly at same time as did (CD25+CD44- DN...