A5065225304- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Cholesterol and Lipid Metabolism
- T-cell and B-cell Immunology
- Rheumatoid Arthritis Research and Therapies
- Diabetes Treatment and Management
- Platelet Disorders and Treatments
- Peroxisome Proliferator-Activated Receptors
- Hemophilia Treatment and Research
- Peptidase Inhibition and Analysis
- T-cell and Retrovirus Studies
- Systemic Lupus Erythematosus Research
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Protease and Inhibitor Mechanisms
- Tuberculosis Research and Epidemiology
- Blood Coagulation and Thrombosis Mechanisms
- Diagnosis and treatment of tuberculosis
- Glycosylation and Glycoproteins Research
- Signaling Pathways in Disease
- Immune Cell Function and Interaction
- Cancer, Lipids, and Metabolism
- Low-power high-performance VLSI design
- Salivary Gland Disorders and Functions
- Antioxidant Activity and Oxidative Stress
Chugai Pharma (United States)
1991-2022
JW Pharmaceutical (South Korea)
2016
Shin Nippon Biomedical Laboratories (Japan)
2016
Ureshino Medical Center
2001-2015
National Hospital Organization
2014
National Institutes of Health
2007
Fujitsu (Japan)
1982-2006
The University of Tokyo
1993-2003
City University of Seattle
2002
Osaka City University
1984-2002
An anti–glypican 3/CD3 bispecific T cell–redirecting antibody (ERY974) is a promising therapeutic agent for solid tumors.
The cellular basis of the in vitro and vivo T cell responses to Staphylococcus enterotoxin B (SEB) has been investigated. proliferation cytotoxicity V beta 8.1,2+,CD4+ CD8+ cells were observed response SEB. In primary assays, CD4+ from control spleens more active than their counterparts, however, derived SEB-primed mice, dominant SEB-specific cytotoxicity. priming with SEB abrogated despite fact that these exist significant number. This anergy occurred only but not cells. These findings...
We show in this manuscript that expression of the mRNA for sterol regulatory element-binding protein-2 (SREBP-2) is regulated by cellular level cultured HeLa cells. have cloned 5′-flanking region gene encoding human SREBP-2. Characterization shows minimum 50-base pair segment, which contains a 10-base element 1 (SRE-1) identical to one LDL receptor promoter, confers responsiveness when fused luciferase reporter gene. Enforced truncated SREBP-2 protein (amino acid residues 1-481) also...
Macrophage scavenger receptors mediate the recognition of a wide range negatively charged macromolecules including modified low density lipoproteins (LDL).Truncated bovine lacking residues 330-342, which include conserved lysine cluster collagen-like domain, were unable to degrade LDL in spite their expression on cell surface.Substitution 337 into alanine abolished acetyl-LDL degradation and binding at 37 "C, but did not abolish 4 "C binding.In contrast, substitution more than 2 lysines this...
Modification of proteins by long‐term incubation with glucose leads to the formation advanced glycation end products (AGE). Recent immunological demonstration presence AGE in several human tissues suggests that they may be involved aging, diabetic complications and atherosclerosis. are taken up macrophages via receptor, which is similar macro‐phage scavenger receptor (MSR). In present study, we examined whether MSR could mediate endocytic uptake using Chinese hamster ovary (CHO) cells...
Emicizumab, a humanised bispecific antibody recognising factors (F) IX/IXa and X/Xa, can accelerate FIXa-catalysed FX activation by bridging FIXa in manner similar to FVIIIa. However, details of the emicizumab-antigen interactions have not been reported so far. In this study, we first showed surface plasmon resonance analysis that emicizumab bound FIX, FIXa, FX, FXa with moderate affinities (KD = 1.58, 1.52, 1.85, 0.978 µM, respectively). We next immunoblotting recognised antigens' epidermal...
Sodium/glucose cotransporter 2 (SGLT2) is the predominant mediator of renal glucose reabsorption and an emerging molecular target for treatment diabetes. We identified a novel potent selective SGLT2 inhibitor, tofogliflozin (CSG452), examined its efficacy pharmacological properties as antidiabetic drug. Tofogliflozin competitively inhibited in cells overexpressing SGLT2, <i>K</i><sub>i</sub> values human, rat, mouse inhibition were 2.9, 14.9, 6.4 nM, respectively. The selectivity toward...
Significance Glucagon-like peptide-1 receptor agonists have become established as a leading class of diabetes medications. However, these peptide-based drugs are administered by subcutaneous injection or, in one case, complex oral dosing regimen. We now report the discovery LY3502970, potent and selective small-molecule GLP-1R agonist. LY3502970 exhibits preclinical pharmacology equivalent to marketed injectable agonist possesses pharmacokinetic properties compatible with humans....
Dysregulation of the complement system is linked to pathogenesis a variety hematological disorders. Eculizumab, an anti-complement C5 monoclonal antibody, current standard care for paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). However, because high levels in plasma, eculizumab has be administered biweekly by intravenous infusion. By applying recycling technology through pH-dependent binding C5, we generated novel humanized antibody against SKY59,...
The prevalence of antibodies to human T lymphotropic virus type I (HTLV-I) was studied in patients with primary Sjögren9s syndrome. Thirteen 36 serum samples were positive by enzyme linked immunosorbent assay (ELISA) and particle agglutination for HTLV-I confirmed western blotting. presence may signify an carrier state. These had a high occurrence extraglandular manifestations such as uveitis, myopathy, recurrent fever compared who did not have HTLV-I. Patients increased spontaneous...
Tofogliflozin, a highly selective inhibitor of sodium/glucose cotransporter 2 (SGLT2), induces urinary glucose excretion (UGE), improves hyperglycemia and reduces body weight in patients with Type diabetes (T2D). The mechanisms tofogliflozin on reduction were investigated detail obese diabetic animal models.Diet-induced (DIO) rats KKAy mice (a mouse model obesity) fed diets containing tofogliflozin. Body weight, composition, biochemical parameters metabolic evaluated.In DIO was administered...
Although inhibition of renal sodium-glucose co-transporter 2 (SGLT2) has a stable glucose-lowering effect in patients with type diabetes, the SGLT2 on dysfunction diabetes remains to be determined. To evaluate renoprotective more precisely, we compared effects tofogliflozin (a specific inhibitor) those losartan (an angiotensin II receptor antagonist) function and beta-cell db/db mice.The 8-week or treatment were investigated mice by quantitative image analysis glomerular size, mesangial...
ABSTRACT Hypoparathyroidism is a disease of chronic hypocalcemia and hyperphosphatemia due to deficiency parathyroid hormone (PTH). PTH analogs the are interest as potential therapies. Accordingly, we examined pharmacological properties long-acting analog, [Ala1,3,12,18,22, Gln10,Arg11,Trp14,Lys26]-PTH(1-14)/PTHrP(15-36) (LA–PTH) in thyroparathyroidectomized (TPTX) rats, model HP, well normal monkeys. In TPTX single intravenous administration LA-PTH at dose 0.9 nmol/kg increased serum...
Emicizumab, a factor (F) VIIIa-function mimetic bispecific antibody (BsAb) to FIXa and FX, has become an indispensable treatment option for people with hemophilia A (PwHA). However, small proportion of PwHA still experience bleeds even under emicizumab prophylaxis, as observed in the long-term outcomes clinical studies. more potent BsAb may be desirable such patients.
The mechanisms of TSH-induced growth stimulation thyrocytes in vivo have yet to be elucidated. We examined the antiapoptotic effect TSH toward Fas antigen-mediated apoptosis thyrocytes. antigen was expressed on approximately 40% unstimulated thyrocytes, and expression significantly inhibited by addition a dose-dependent manner. Treatment with 8-bromo-cAMP mimicked TSH, suggesting that inhibitory mediated activating protein kinase A. In contrast, treatment either interleukin-1 beta (IL-1...
Abstract Parathyroid hormone (PTH) is essential for calcium homeostasis and its action mediated by the PTH type 1 receptor (PTHR1), a class B G-protein-coupled receptor. Hypoparathyroidism osteoporosis can be treated with injections; however, no orally effective analogue available. Here we show that PCO371 novel, active small molecule acts as full agonist of PTHR1. does not affect 2 (PTHR2), analysis using PTHR1–PTHR2 chimeric receptors indicated Proline 415 PTHR1 critical PCO371-mediated...