A5101644175- Reproductive Biology and Fertility
- Sperm and Testicular Function
- PI3K/AKT/mTOR signaling in cancer
- Cholesterol and Lipid Metabolism
- Epigenetics and DNA Methylation
- Ovarian function and disorders
- Peroxisome Proliferator-Activated Receptors
- Tuberous Sclerosis Complex Research
- Animal Genetics and Reproduction
- RNA Research and Splicing
- Antioxidant Activity and Oxidative Stress
- Monoclonal and Polyclonal Antibodies Research
- Hemophilia Treatment and Research
- Liver Disease Diagnosis and Treatment
- Genetic Syndromes and Imprinting
- Diet and metabolism studies
- Reproductive Health and Technologies
- Metabolism, Diabetes, and Cancer
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Sepsis Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Cancer-related gene regulation
- Pluripotent Stem Cells Research
- Reproductive Physiology in Livestock
Tokyo University of Agriculture
2016-2025
Shizuoka Medical Center
2021
National Hospital Organization
2017-2020
Boston Children's Hospital
2016-2018
Howard Hughes Medical Institute
2016-2018
University of Michigan–Ann Arbor
2011-2017
Jichi Medical University Hospital
2013-2017
Jichi Medical University
2001-2017
Harvard Stem Cell Institute
2017
Harvard University
2017
Diabetic nephropathy (DN) is among the most lethal complications that occur in type 1 and 2 diabetics. Podocyte dysfunction postulated to be a critical event associated with proteinuria glomerulosclerosis glomerular diseases including DN. However, molecular mechanisms of podocyte development DN are not well understood. Here we have shown activity mTOR complex (mTORC1), kinase senses nutrient availability, was enhanced podocytes diabetic animals. Further, podocyte-specific mTORC1 activation...
The RNA binding protein, LARP1, has been proposed to function downstream of mTORC1 regulate the translation 5'TOP mRNAs such as those encoding ribosome proteins (RP). However, roles LARP1 in are controversial and its regulatory mTORC1-mediated remain unclear. Here we show that is a direct substrate Akt/S6K1. Deep sequencing LARP1-bound reveal non-phosphorylated interacts with both 5' 3'UTRs RP inhibits their translation. Importantly, phosphorylation by Akt/S6K1 dissociates it from 5'UTRs...
Podocyte depletion leads to glomerulosclerosis, but whether an impaired capacity of podocytes respond hypertrophic stress also causes glomerulosclerosis is unknown. We generated transgenic Fischer 344 rats that express a dominant negative AA-4E-BP1 transgene driven by the podocin promoter; member mammalian target rapamycin complex 1 (mTORC1) pathway, 4E-BP1 modulates cap-dependent translation, which key determinant cell’s response nutrients and growth factors. rat expressed had normal kidney...
Mammalian target of rapamycin (mTOR) is a kinase that plays key role in wide array cellular processes and exists two distinct functional complexes, mTOR complex 1 (mTORC1) 2 (mTORC2). Although mTORC2 primarily activated by growth factors, mTORC1 regulated numerous extracellular intracellular signals such as nutrients, redox. Previous study has shown cysteine oxidants sufficiently activate activity under amino acid-depleted conditions reducing agent effectively suppresses acid-induced...
Autophagy has been implicated in a number of physiological processes important for human heath and disease. involves the formation double-membrane cytosolic vesicle, an autophagosome. Central to autophagosome is ubiquitin-like protein autophagy-related (Atg)8 (microtubule-associated 1 light chain 3/LC3 mammalian cells). Following autophagy induction, Atg8 shows greatest change expression any proteins required autophagy. The magnitude is, part, controlled by amount Atg8; thus, controlling...
2-Nitropropane dioxygenase, purified to homogeneity from Hansenula mrakii (IFO 0895), has a molecular weight of approximately 62,000 and consists two subunits nonidentical in (39,000 25,000). Stoichiometrical studies the results obtained with 18O2 showed that 2 atoms oxygen are incorporated into molecules acetone formed 2-nitropropane. In addition 2-nitropropane, nitroethane, 3-nitro-2-pentanol, 1-nitropropane oxidatively dentrified. The enzyme, which exhibits absorption maxima at 274, 370,...
To understand mammalian active DNA demethylation, various methods have been developed to map the genomic distribution of demethylation intermediates 5-formylcysotine (5fC) and 5-carboxylcytosine (5caC). However, majority these requires a large number cells begin with. In this study, we describe low-input methylase-assisted bisulfite sequencing (liMAB-seq ) single-cell MAB-seq (scMAB-seq), capable profiling 5fC 5caC at genome scale using ∼100 single cells, respectively. liMAB-seq analysis...
Abstract Sterol regulatory element-binding proteins (SREBPs) are transcription factors that regulate various genes involved in cholesterol and fatty acid synthesis, playing a central role lipid metabolism regulation vivo. SREBP-1c activity is significantly elevated the liver under conditions of obesity, disease, type II diabetes, while suppression has been shown to alleviate these symptoms. Consequently, targeting considered potential therapeutic approach for conditions. In this study, we...
Computed tomography (CT) offers high-resolution, three-dimensional imaging, making it particularly valuable for assessing complex structures, such as the head, especially when conventional radiography and endoscopy are insufficient a definitive diagnosis. Herein, we present two cases of equine cranial disorders resulting from congenital malformations. In case 1, which had dentigerous cyst, CT images confirmed location ectopic tooth within skull detailed fistula tube. 2, temporohyoid...
Antioxidants have been proposed to antiatherogenic potential by their inhibition of low density lipoprotein (LDL) oxidation. Here, we report an antioxidant, BO-653 (2,3-dihydro-5-hydroxy-2,2-dipentyl-4,6-di- tert- butylbenzofuran), designed exhibit antioxidative potency comparable that α-tocopherol, but yet possess a high degree lipophilicity probucol. exhibits affinity for LDL and is well distributed in aortic vessels vivo . In atherosclerosis models rabbits mice, has shown be able suppress...
( Cancer Sci 2010; 101: 759–766) Matrix metalloproteinase (MMP)‐9, the 92‐kDa type IV collagenase, contributes to tumor invasion and metastases, strategies down‐regulate its expression could ultimately be of clinical utility. A pyrrole‐imidazole (PI) polyamide that targets activator protein‐1 (AP‐1)‐binding site MMP‐9 promoter was designed synthesized as a gene‐silencing agent for metastases. The product showed selective DNA binding ability. PI significantly inhibited MMP‐9’s mRNA...