A5036716509- Renin-Angiotensin System Studies
- Hormonal Regulation and Hypertension
- Angiogenesis and VEGF in Cancer
- Apelin-related biomedical research
- Eicosanoids and Hypertension Pharmacology
- Cardiovascular Function and Risk Factors
- Mesenchymal stem cell research
- Renal and related cancers
- Cardiac Imaging and Diagnostics
- Nitric Oxide and Endothelin Effects
- Cardiac Valve Diseases and Treatments
- TGF-β signaling in diseases
- Tissue Engineering and Regenerative Medicine
- Cell Adhesion Molecules Research
- Liver Disease Diagnosis and Treatment
- Cardiac Fibrosis and Remodeling
- Adipose Tissue and Metabolism
- Phonocardiography and Auscultation Techniques
- Complement system in diseases
- RNA Interference and Gene Delivery
- Atherosclerosis and Cardiovascular Diseases
- Cardiomyopathy and Myosin Studies
- Receptor Mechanisms and Signaling
- Cancer, Hypoxia, and Metabolism
- Coronary Interventions and Diagnostics
Nihon University
2015-2024
University of Pittsburgh
2024
Fukuda Denshi (Japan)
1996-2021
Weatherford College
2021
Advanced Research Institute
2007-2013
GTx (United States)
2010
NTT (Japan)
2009
Hypertension Institute
2008
Kyoto University
2008
National Cerebral and Cardiovascular Center
2008
When mature adipocytes are subjected to an in vitro dedifferentiation strategy referred as ceiling culture, these can revert a more primitive phenotype and gain cell proliferative ability. We refer cells dedifferentiated fat (DFAT) cells. In the present study, we examined multilineage differentiation potential of DFAT obtained from adipose tissues 18 donors exhibited fibroblast-like morphology sustained high activity. Flow cytometric analysis revealed that comprised highly homogeneous...
We recently reported that mature, adipocyte derived, dedifferentiated fat cells show high proliferative activity and multilineage differentiation potential. In the current study we investigated whether such could differentiate into a smooth muscle cell lineage contribute to bladder tissue regeneration in mouse injury model.Human derived were cultured for 1 week under conditions favorable immunostained alpha-smooth actin. The expression of marker genes differentiating was measured by...
We have demonstrated that mesenchymal cells from spontaneously hypertensive rats genetically express complement 3 (C3). Mature tubular epithelial can undergo epithelial-to-mesenchymal transition (EMT) is linked to the pathogenesis of renal fibrosis and injury. In this study, we investigated contribution C3 in EMT renin-angiotensin (RA) systems associated with hypertension. C3a induced mouse TCMK-1 cells, which displayed increased expression renin Krüppel-like factor 5 (KLF5) nuclear...
Pyrrole-imidazole (Py-Im) polyamides are nuclease-resistant novel compounds that inhibit gene expression by binding to the minor groove of DNA. A Py-Im polyamide targets rat TGF-beta1 was designed as a gene-silencing agent for progressive renal diseases, and distribution effects this on injury were examined in Dahl-salt sensitive (Dahl-S) rats. For identification transcription factor elements activation gene, recombinant reporter plasmids transfected into HEK-293 cells, promoter activity...
Angiotensin II (Ang II) receptor blocker (ARB) has been reported to have protective effects on the cardiovascular system independent of blood pressure reduction. Endothelial progenitor cells (EPCs) play a significant role in neovascularization ischemic tissue. The average lifespan EPCs was recently be shortened by oxidative stress and regulated anti-oxidative mechanisms. It that are present peripheral ability repair damage. We investigated an ARB, candesartan, EPC function oxidation...
// Kosuke Saito 1, 2 , Tomohiko Asai 3 Kyoko Fujiwara Junki Sahara Haruhisa Koguchi 4 Noboru Fukuda 5 Miki Suzuki-Karasaki 6 Masayoshi Soma Yoshihiro 7 1 Innovative Therapy Research Group, Nihon University Institute of Medical Science, Tokyo, Japan Division General Medicine, Department School Physics, College Science and Technology, University, The National Advanced Industrial Tsukuba, Nephrology Hypertension Endocrinology, Dermatology, Surugadai Hospital, Saitama, Physiology, Biomedical...
Abstract Recently, there has been noteworthy progress in the field of cardiac regeneration therapy. We previously reported that brown adipose tissue (BAT) contained progenitor cells were relevant to damaged myocardium. In this study, we found CD133-positive, but not c-Kit- or Sca-1-positive, BAT differentiated into cardiomyocytes (CMs) with a high frequency. Moreover, CD133+ tissue-derived (BATDCs) effectively induced bone marrow (BMCs) CMs. BMCs are considered have greatest potential as...
( Cancer Sci 2010; 101: 759–766) Matrix metalloproteinase (MMP)‐9, the 92‐kDa type IV collagenase, contributes to tumor invasion and metastases, strategies down‐regulate its expression could ultimately be of clinical utility. A pyrrole‐imidazole (PI) polyamide that targets activator protein‐1 (AP‐1)‐binding site MMP‐9 promoter was designed synthesized as a gene‐silencing agent for metastases. The product showed selective DNA binding ability. PI significantly inhibited MMP‐9’s mRNA...
Objectives: To examine the effects of mature adipocyte‐derived dedifferentiated fat (DFAT) cell transplantation on urethral tissue regeneration and sphincter function. Methods: Sixteen female Sprague–Dawley rats underwent vaginal distension (VD) for 3 h. Subsequently, green fluorescence protein (GFP)‐labeled DFAT cells (1 × 10 6 in 20 µL saline, group, n = 8) or saline (20 µL, control were injected into paraurethral connective tissue. Two weeks following VD, leak point pressure (LPP) was...
Abstract —Production of angiotensin II (Ang II) in spontaneously hypertensive rats (SHR)-derived vascular smooth muscle cells (VSMC) has now been investigated. A nonpeptide antagonist (CV-11974) Ang type 1 receptors inhibited basal DNA synthesis VSMC from SHR, but it had no effect on Wistar-Kyoto (WKY) rats. II-like immunoreactivity, determined by radioimmunoassay after HPLC, was readily detected conditioned medium and extracts SHR-derived VSMC, whereas virtually undetectable WKY...
Aim: To investigate the mechanisms underlying pro-angiogenic effects of statin, atorvastatin were investigated on expression angiogenic factors in ischemic hindlimbs rats. The function and number endothelial progenitor cells (EPCs) hypertensive rats.Methods: Hindlimb ischemia rats administered 10 or 30 mg/kg/day orally for 2 weeks. Angiogenesis was evaluated by a laser Doppler Isolectin-B4 immunostaining. expressions VEGF, IL-8, angiopoietin (Ang)-1, Ang-2, eNOS, hemoxidase (HO)-1 Western...
Although the use of drug-eluting stents (DESs) has been shown to limit neointima hyperplasia, currently available DESs may adversely affect re-endothelialization. To evaluate whether a novel gene silencer pyrrole–imidazole (PI) polyamide targeting transforming growth factor (TGF)-β1 is candidate agent for DESs, we examined effects PI TGF-β1 promoter on neointimal formation in rat carotid artery after balloon injury. was designed span boundary AP-1 binding site promoter. After inducing injury...
Endothelial progenitor cells (EPCs) induce neovascularization and repair vascular damage. We have demonstrated that EPC function is impaired in hypertensive rats with increases oxidative stress angiotensin II receptor blockers improved the of EPCs. In this study, we investigated basal functions normotensive control subjects patients essential hypertension effect losartan on patients. Eighteen 36 who were undergoing treatment participated study. Hypertensive randomly selected to receive 50mg...
We report a preclinical study of pyrrole-imidazole (PI) polyamide that targets the human transforming growth factor (hTGF)-β1 gene as novel transcriptional silencer in common marmoset primate model. designed and then synthesized PI polyamides to target hTGF-β1 promoter. examined effects seven (GB1101-1107) on expression mRNA stimulated with phorbol 12-myristate 13-acetate (PMA) vascular smooth muscle cells. GB1101, GB1105 GB1106 significantly inhibited expression. GB1101 for hypertrophic...
Two distinct subtypes of angiotensin (Ang) II receptors, type 1 (AT ) and 2 ), have been identified. Vascular smooth muscle cells (VSMCs) usually express AT receptor. To elucidate the direct effects receptor on in VSMCs, we transfected gene into cultured rat VSMCs. Overexpression significantly decreased expression 1a at both mRNA protein levels presence absence Ang increased bradykinin inducible NO Bradykinin B antagonist HOE–140 synthase inhibitor N ω -nitro- l -arginine methyl ester...