A5101641995- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Polyamine Metabolism and Applications
- Metabolism and Genetic Disorders
- Biopolymer Synthesis and Applications
- Cancer Research and Treatments
- Biochemical and Molecular Research
- Enzyme Catalysis and Immobilization
- Microbial bioremediation and biosurfactants
- Asthma and respiratory diseases
- Chemical Synthesis and Analysis
- Chemical Reactions and Isotopes
- Protein Structure and Dynamics
- IL-33, ST2, and ILC Pathways
- Selenium in Biological Systems
- Enzyme Production and Characterization
- Extracellular vesicles in disease
- Sulfur Compounds in Biology
- Microbial metabolism and enzyme function
- Microbial Metabolic Engineering and Bioproduction
- Enzyme function and inhibition
- Mass Spectrometry Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Porphyrin Metabolism and Disorders
- Tryptophan and brain disorders
Nihon University
2014-2024
Kyoto University
1989-2008
Kansai University
1997-2008
Kyoto University Institute for Chemical Research
1987-2008
Yamaguchi University
2008
Indian Institute of Science Bangalore
2008
The University of Tokyo
2008
Kyoto Bunkyo University
1981-1998
Instituto de Investigaciones Químicas
1992-1998
Okayama University
1997
We have found a novel enzyme that exclusively decomposes L-selenocysteine into L-alanine and H2Se in various mammalian tissues, named it selenocysteine lyase. The from pig liver has been purified to homogeneity. It molecular weight of approximately 85,000, contains pyridoxal 5'-phosphate as coenzyme. Its maximum reactivity is at about pH 9.0. Balance studies showed 1 mol converted equimolar amounts alanine H2Se. following amino acids are insert: L-cysteine, L-serine, L-cysteine sulfinate,...
L-Lysine a-oxidase from Trichoderma vi& Y244-2 has been purified to homogeneity.The enzyme shows absorption maxima at 277, 388, and 466 nm a shoulder around 490 n m contains 2 mol of FAD/ enzyme.The molecular weight approximately 116,000 consists two subunits identical in (about 56,000).In addition L-lysine, L-ornithine, L-phenylalanine, L-tyrosine, L- arginine, L-histidine are oxidized by the lesser extent.Several lysine analogs such as &hydroxylysine efficiently.Balance studies showed that...
The distribution of bacterial leucine dehydrogenase (L-leucine:NAD+ oxidoreductase, deaminating, EC 1.4.1.9) was investigated, and Bacillus sphaericus (IFO 3525) found to have the highest activity enzyme. Leucine dehydrogenase, which purified homogeneity crystallized from B. sphaericus, has a molecular weight about 245,000 consists six identical subunits (Mr = 41,000). enzyme catalyzes oxidative deamination L-leucine, L-valine, L-isoleucine, L-norvaline, L-alpha-aminobutyrate, L-norleucine,...
We describe a simple method for enzymatic synthesis of L and D amino acids from alpha-keto with Escherichia coli cells which express heterologous genes. L-amino were produced thermostable acid dehydrogenase formate (FDH) ammonium only an intracellular pool NAD+ the regeneration NADH. constructed plasmids containing, in addition to FDH gene, genes dehydrogenases, including i.e., leucine dehydrogenase, alanine phenylalanine dehydrogenase. L-Leucine, L-valine, L-norvaline, L-methionine,...
A new enzyme, DL-2-haloacid dehalogenase, was isolated and purified to homogeneity from the cells of Pseudomonas sp. strain 113. This enzyme catalyzed non-stereospecific dehalogenation both optical isomers 2-chloropropionate through an SN2 type reaction; L- D-lactates were formed D- L-2-chloropropionates, respectively. The acted on 2-halogenated aliphatic carboxylic acids whose carbon chain lengths less than five. It also dehalogenated trichloroacetate form oxalate showed maximum activity at...
D-Amino acid aminotransferase, purified to homogeneity and crystallized from Bacillus sphaericus, has a molecular weight of about 60,000 consists two subunits identical in (30,000). The enzyme exhibits absorption maxima at 280, 330, 415 nm, which are independent the pH (5.5 10.0), contains 2 mol pyridoxal 5'-phosphate per enzyme. One pyridoxal-5'-P, absorbing is bound an aldimine linkage epsilon-amino group lysine residue protein, released by incubation with phenylhydrazine yield...
MicroRNAs (miRNAs) may facilitate cell-to-cell communication via extracellular vesicles (EVs). The biological roles of miRNAs in EVs on allergic airway inflammation are unclear.Airway-secreted (AEVs) were isolated from bronchoalveolar lavage fluid (BALF) control and house-dust mite (HDM) allergen-exposed HDM-sensitized mice. expression AEVs or mRNAs lung tissue was analysed using miRNA microarray.The amount AEV increased 8.9-fold BALF HDM-exposed mice compared with that sham-control HDM...
Although the pathogenesis of pulmonary fibrosis remains unclear, it is known to involve epithelial injury and epithelial-mesenchymal transformation (EMT) as a consequence cigarette smoke (CS) exposure. Moreover, smoking deposits iron in mitochondria alveolar cells. Iron overload causes Fenton reaction, leading reactive oxygen species (ROS) production, ROS leakage from induces cell inflammation lungs. Nevertheless, mechanisms underlying metabolism are yet be elucidated. In this study, we...
D-Amino acid aminotransferase was found in several thermophilic Bacillus species and purified to homogeneity from the best producer, sp. YM-1, which newly isolated a sauna dust. The enzyme has molecular weight of about 62,000 consists two subunits identical (30,000). It catalyzes transamination between various D-amino acids alpha-keto acids, although substrate specificity is narrower than mesophile, sphaericus (Yonaha, K., Misono, H., Yamamoto, T., Soda, K. (1975) J. Biol. Chem. 250,...
meso-a,eDiaminopimelate D-dehydrogenase, which has been purified to homogeneity from the extract of Bacillus sphaericus IF0 3626, a molecular weight about 80,000 and consists two subunits identical in (approximately 40,000).The enzyme high substrate specificity.In addition mso-a,diaminopimelate, lanthionine is deaminated by far lesser extent.NADP+ exclusive cofactor.The pH optima were at 10.6 for deamination meso-a,€-diaminopimelate 7.6 its amination.L D isomers a,ediaminopimelate...
A high activity of meso-alpha-epsilon-diaminopimelate dehydrogenase was found in extracts Bacillus sphaericus, Brevibacterium sp., Corynebacterium glutamicum, and Proteus vulgaris among bacteria tested. B. sphaericus IFO 3525, which the enzyme is most abundant, chosen to study reaction. The not induced by addition growth medium. reaction product isolated identified as alpha-amino-epsilon-ketopimelate a comparison properties its 2,4-dinitrophenylhydrazone with those an authentic sample silica...
2-Nitropropane dioxygenase, purified to homogeneity from Hansenula mrakii (IFO 0895), has a molecular weight of approximately 62,000 and consists two subunits nonidentical in (39,000 25,000). Stoichiometrical studies the results obtained with 18O2 showed that 2 atoms oxygen are incorporated into molecules acetone formed 2-nitropropane. In addition 2-nitropropane, nitroethane, 3-nitro-2-pentanol, 1-nitropropane oxidatively dentrified. The enzyme, which exhibits absorption maxima at 274, 370,...
We found glutamate racemase activity in cell extracts of Bacillus subtilis IFO 3336, which abundantly produces poly-γ-glutamate. The highest was obtained the early stationary phase growth. purified to homogeneity. enzyme a monomer with molecular mass about 30 kDa and required no cofactor. It almost exclusively catalyzed racemization glutamate; other amino acids, including alanine aspartate but not homocysteinesulfinate, were inactive as either substrates or inhibitors. Although Vmax value...
Aspartate racemase from Streptococcus thermophilus contains no pyridoxal 5"phosphate or other cofactors such as FAD, NAD+, and metal ions.It was affected by neither carbonyl reagents hydroxylamine nor sodium borohydride but strongly inhibited iodoacetamide thiol reagents.Aspartate, cysteate, cysteine sulfinate were the only substrates.The K , values for L-and D-aspartate 35 8.7 mM, respectively.The enzyme catalyzed exchange of a-hydrogen substrate with solvent hydrogen.Racemization...
Two novel hydrolytic dehalogenases, thermostable L-2-haloacid dehalogenase (L-DEX) inducibly synthesized by 2-chloropropionate (2-CPA) and nonthermostable DL-2-haloacid (DL-DEX) induced 2-chloroacrylate, were purified to homogeneity from Pseudomonas sp. strain YL. DL-DEX consisted of a monomer with molecular weight about 36,000 catalyzed the dehalogenation L D isomers 2-CPA produce D- L-lactates, respectively. It acted on 2-haloalkanoic acids carbon chain length 2 4. The maximum activity...
The gene for thermostable D-amino acid aminotransferase from a thermophile, Bacillus species YM-1 was cloned and expressed efficiently in Escherichia coli. entire covalent structure of the enzyme determined nucleotide sequence mostly confirmed by amino sequences tryptic peptides product. polypeptide is composed 282 residues with calculated molecular weight 32,226. Comparison primary those various proteins registered protein data bank revealed significant homology between L-branched chain E....
A nitroalkane-oxidizing enzyme, which was inducibly formed by addition of nitroethane to the medium purified homogeneity from an extract Fusarium oxysporum (IFO 5942) with overall yield about 20%. The enzyme catalyzed oxidative denitrification 1-nitropropane as follows: CH2(NO2)CH2CH3 + O2 H2O leads OHCCH2CH3 HNO2 H2O2. In 1-nitropropane, 3-nitro-2-pentanol, 2-nitropropane, and nitrocyclohexane are good substrates; is designated "nitroalkane oxidase" (EC class 1.7.3). has a molecular weight...
Inhaled corticosteroids enhance airway epithelial barrier integrity. However, the mechanism by which they accomplish this is unclear. Therefore, we investigated steroid-inducible genes and signaling pathways that were involved in enhancing A human bronchial cell line (16HBE cells) was cultured with 10−6 M dexamethasone (DEX) for 3 days to After measuring transepithelial electrical resistance (TER) paracellular permeability, extracted total RNA from 16HBE cells performed microarray pathway...
DL-2-Haloacid dehalogenase from Pseudomonas sp. strain 113 (DL-DEX) catalyzes the hydrolytic dehalogenation of both D- and L-2-haloalkanoic acids to produce corresponding L- D-2-hydroxyalkanoic acids, respectively, with inversion C2 configuration. DL-DEX is a unique enzyme: it acts on chiral carbon substrate uses enantiomers as equivalent substrates. We have isolated sequenced gene encoding DL-DEX. The open reading frame consists 921 bp 307 amino acid residues. No sequence similarity between...
The gene encoding aspartate aminotransferase of a thermophilic Bacillus species, YM-2, has been cloned and expressed efficiently in Escherichia coli. primary structure the enzyme was deduced from nucleotide sequences confirmed mostly by amino acid tryptic peptides. consists 1,176 base pairs protein 392 residues; molecular mass subunit is estimated to be 42,661 daltons. active site lysyl residue that binds coenzyme, pyridoxal phosphate, identified as Lys-239. Comparison sequence with those...
Asthma is one of the most common immunological diseases and characterized by airway hyperresponsiveness (AHR), mucus overproduction, eosinophilia. Although mouse models have provided insight into mechanisms which type-2 cytokines induce asthmatic inflammation, differences between rodent human immune systems hamper efforts to improve understanding allergic diseases. In this study, we aim establish a preclinical animal model inflammation using humanized IL-3/GM-CSF or IL-3/GM-CSF/IL-5 Tg...
We have found that Pseudomonas putida ATCC 17642 cells grown in a medium containing D-threonine as the sole nitrogen source produce an enzyme catalyzes epimerization of threonine. Proton nuclear magnetic resonance analysis reaction deuterium oxide clearly showed from L- to D-allo-threonine and also D- L-allo-threonine. This is first example was shown epimerize The has been purified homogeneity, which by polyacrylamide gel electrophoresis. molecular weight about 82,000 consists two subunits...