A5052893785- Coronary Interventions and Diagnostics
- Aquaculture disease management and microbiota
- Cardiac Imaging and Diagnostics
- Peripheral Artery Disease Management
- Aquaculture Nutrition and Growth
- Ubiquitin and proteasome pathways
- Cerebrovascular and Carotid Artery Diseases
- Gastric Cancer Management and Outcomes
- Vibrio bacteria research studies
- Metastasis and carcinoma case studies
- Cancer, Hypoxia, and Metabolism
- Gastrointestinal Tumor Research and Treatment
- Identification and Quantification in Food
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Research and Treatments
- Colorectal Cancer Surgical Treatments
- Animal Virus Infections Studies
- Cardiac Valve Diseases and Treatments
- Pancreatic and Hepatic Oncology Research
- Drug Transport and Resistance Mechanisms
- CRISPR and Genetic Engineering
- Cancer Diagnosis and Treatment
- Virus-based gene therapy research
- Vascular Procedures and Complications
- Cancer, Lipids, and Metabolism
CVPath Institute
2020-2025
Juntendo University Shizuoka Hospital
2024-2025
Kansai Rosai Hospital
2018-2025
Osaka National Hospital
2022-2024
Central Institute for Experimental Animals
2015-2024
Juntendo University Nerima Hospital
2022-2024
Hyogo Medical University
2012-2023
Hyogo University
2022
Hoshigaoka Medical Center
2019-2022
Cook Medical (Denmark)
2021
Background: Cardiac injury is common in patients who are hospitalized with coronavirus disease 2019 (COVID-19) and portends poorer prognosis. However, the mechanism type of myocardial damage associated severe acute respiratory syndrome 2 (SARS-CoV-2) remain uncertain. Methods: We conducted a systematic pathological analysis 40 hearts from dying COVID-19 Bergamo, Italy, to determine mechanisms cardiac injury. divided according presence or absence myocyte necrosis then determined underlying...
Abstract The development of animal models that mimic human allergic responses is crucial to study the pathophysiology disease and generate new therapeutic methodologies. Humanized mice reconstituted with immune systems are essential reactions in vivo expected be useful for studying allergies. However, application this technology allergies has been limited, largely because poor myeloid cells, especially granulocytes mast which responsible mediating diseases, conventional humanized mice. In...
Background. Several animal models for xenogenic (xeno) graft versus host disease (GVHD) have been developed in immunodeficient mice, such as C.B-17-scid and nonobese diabetes (NOD)/severe combined immunodeficiency (SCID), by human peripheral blood mononuclear cell (hPBMC) transplantation. However, these pose problems because they require sublethal total body irradiation of the mice a large number hPBMCs to induce GVHD, timing onset GVHD is also unstable. The aim this study establish improved...
Although embryonic stem (ES) cell–like induced pluripotent (iPS) cells have potential therapeutic applications in humans, they are also useful for creating genetically modified human disease models nonhuman primates. In this study, we generated common marmoset iPS from fetal liver via the retrovirus‐mediated introduction of six transcription factors: Oct‐3/4, Sox2, Klf4, c‐Myc, Nanog, and Lin28. Four to five weeks after introduction, several colonies resembling ES were observed picked...
Device-related thrombus (DRT) after left atrial appendage closure (LAAC) procedures is a rare but potentially serious event. Thrombogenicity and delayed endothelialization play role in the development of DRT. Fluorinated polymers are known to have thromboresistant properties that may favorably modulate healing response an LAAC device.The goal this study was compare thrombogenicity endothelial coverage (EC) between conventional uncoated WATCHMAN FLX (WM) novel fluoropolymer-coated...
BACKGROUND: Cell phenotype switching is increasingly being recognized in atherosclerosis. However, our understanding of the exact stimuli for such cellular transformations and their significance human atherosclerosis still evolving. Intraplaque hemorrhage thought to be a major contributor plaque progression part by stimulating influx CD163 + macrophages. Here, we explored hypothesis that macrophages cause through induction proapoptotic endothelial-to-mesenchymal transition (EndMT) within...
BACKGROUND: Hypoattenuated leaflet thickening (HALT) is believed to reflect thrombosis; however, no systematic histological examination of HALT has ever been performed. The aim this study was evaluate findings explanted self-expanding transcatheter aortic bioprosthetic valves from clinical trials and compare microCT suspected with histology valve thrombosis its characterization over time. METHODS: A total 123 were collected through autopsy (n=89) or surgical explant (n=34) 11...
Hypoxia is an essential feature of cancer malignancy, but there are no methods for the routine detection hypoxia-inducible prognostic factors and potential therapeutic targets. We reported previously that hypoxic tumor cells metastatic liver tissue from patients with colorectal (CRC) could be used as 'in vivo' hypoxia culture model. Several genes were identified using this Among them, one glycolytic enzyme was special interest. There currently increasing attention on enzymes targets due to...
Vascular calcification (VC) is concomitant with atherosclerosis, yet it remains uncertain why rupture-prone high-risk plaques do not typically show extensive calcification. Intraplaque hemorrhage (IPH) deposits erythrocyte-derived cholesterol, enlarging the necrotic core and promoting plaque development. Pro-atherogenic CD163+ alternative macrophages engulf hemoglobin:haptoglobin (HH) complexes at IPH sites. However, their role in VC has never been examined to our knowledge. Here we show,...
While coronary artery disease remains a major cause of death, it is preventable. Therefore, the focus needs to shift early detection and prevention atherosclerosis. Asymptomatic atherosclerosis widely termed subclinical atherosclerosis, which an indicator atherosclerotic burden, understanding this important because timely intervention could prevent future cardiovascular morbidity mortality. We histologically recognize earliest lesion as pathological intimal thickening, characterized by...
In spinal cord injury, axonal disruption results in motor and sensory function impairment. The evaluation of fibers is essential to assess the severity injury efficacy any treatment protocol, but conventional methods such as tracer injection brain parenchyma are highly invasive require histological evaluation, precluding clinical applications. Previous advances magnetic resonance imaging technology have led development diffusion tensor tractography (DTT) a potential modality perform vivo...
Hepatic uptake and biliary excretion of olmesartan, a new angiotensin II blocker, were investigated in vitro using human hepatocytes, cells expressing transporters canalicular membrane vesicles, vivo Eisai hyperbilirubinemic rats (EHBR), inherited multidrug resistance-associated protein (mrp2)-deficient rats. The by hepatocytes reached saturation with Michaelis constant (<i>K</i><sub>m</sub>) 29.3 ± 9.9 μM. Both Na<sup>+</sup>-dependent Na<sup>+</sup>-independent olmesartan observed....
Viable and stable human cancer cell lines animal models combined with adequate clinical information are essential for future advances in research patient care. Conventional vitro commonly available; however, they lack detailed on the from which originate, including disease phenotype drug sensitivity. Patient-derived xenografts (PDX) (so-called 'cancer xenopatients') a promising advance that may accelerate development of anticancer therapies. We established 61 PDX 116 surgically removed tumor...
The hepatoma-derived cell line HepaRG is regarded as an in vitro model of drug metabolism because fully differentiated cells demonstrate functional metabolic responses comparable to those primary human hepatocytes. Recently, it was demonstrated that the 3D culture enhanced their functions and toxicological responses. We approached mechanisms underlying these enhancement effects.We compared 2D-cultured with 3D-cultured spheroids gene expression patterns functions. In present study, we...
One of the main reasons for cancer treatment resistance is existence stem-like cells (CSCs). Here, we elucidated relationship between low proteasome activity (LPACs) and CSCs.The human colorectal cell lines HCT116, SW480, DLD1, KM12SM were engineered to stably express a green fluorescent molecule fused degron ornithine decarboxylase, resulting in an accumulation fluorescence LPACs. LPACs isolated by flow cytometry. Treatment (radio- chemotherapy) capacity act as CSCs analyzed. Microarray...