A5059264330- Cancer-related gene regulation
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Epigenetics and DNA Methylation
- Atrial Fibrillation Management and Outcomes
- Platelet Disorders and Treatments
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Vaccine Coverage and Hesitancy
- SARS-CoV-2 detection and testing
- Blood Coagulation and Thrombosis Mechanisms
- vaccines and immunoinformatics approaches
- Blood properties and coagulation
- Monoclonal and Polyclonal Antibodies Research
- Respiratory viral infections research
- Chronic Myeloid Leukemia Treatments
- Hemophilia Treatment and Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Mass Spectrometry Techniques and Applications
- Cancer Research and Treatments
- Acute Myeloid Leukemia Research
Keio University
2015-2025
Institute of Science Tokyo
2024
Keio University Hospital
2001-2023
The Jikei University Hospital
2012
Central Institute for Experimental Animals
2008-2011
Shimadzu (Japan)
2009
Hamamatsu University School of Medicine
2009
Saitama Medical University
2005-2008
Center for Rheumatology
2004-2005
Tokyo University of the Arts
2001
To determine the incidence, natural course, and severity of dry eye occurring or worsening after haematopoietic stem cell transplantation (SCT).At a tertiary care hospital, 53 patients undergoing allogeneic autologous SCT followed by at least 180 days follow up were studied prospectively. Examination included grading symptoms eye, evaluation ocular surface, tear break time, Schirmer tests with without nasal stimulation. Meibomian gland secretion was also examined using slit lamp while...
We have developed a mass microscope (mass spectrometry imager with spatial resolution higher than the naked eye) equipped an atmospheric pressure ion-source chamber for laser desorption/ionization (AP-LDI) and quadrupole ion trap time-of-flight (QIT-TOF) analyzer. The optical combined spectrometer permitted us to precisely determine relevant tissue region prior performing imaging (IMS). An ultraviolet tightly focused triplet lens was used achieve high resolution. enables analyze fresh...
Abstract Triple-negative breast cancer (TNBC) chemoresistance hampers the ability to effectively treat patients. Identification of mechanisms driving can lead strategies improve treatment. Here, we revealed that protein arginine methyltransferase-1 (PRMT1) simultaneously methylates D-3-phosphoglycerate dehydrogenase (PHGDH), a critical enzyme in serine synthesis, and glycolytic enzymes PFKFB3 PKM2 TNBC cells. 13C metabolic flux analyses showed PRMT1-dependent methylation these three diverts...
Abstract Effective early-stage markers for predicting which patients are at risk of developing SARS-CoV-2 infection have not been fully investigated. Here, we performed comprehensive serum metabolome analysis a total 83 from two cohorts to determine that the acceleration amino acid catabolism within 5 days disease onset correlated with future severity. Increased levels de-aminated catabolites involved in de novo nucleotide synthesis pathway were identified as early prognostic initial viral...
A questionnaire study was performed among 23,209 dialysis patients in 589 institutions together with a retrospective at Keio University Hospital to determine the risk of malignancy and factors affecting ratio patients. The incidence mortality were 1.4-fold 1.9-fold higher than expected rates general population. Incidence 2.6-fold males females. ratios remarkably high university hospitals, followed by public hospitals. However, there no significant difference between those private hospitals...
Analyses of energy metabolism in human cancer have been difficult because rapid turnover the metabolites and difficulties reducing time for collecting clinical samples under surgical procedures. Utilization xenograft transplantation human-derived colon HCT116 cells spleens superimmunodeficient NOD/SCID/IL-2Rγ(null) (NOG) mice led us to establish an experimental model hepatic micrometastasis solid tumor, whereby analyses tissue sections collected by snap-frozen procedures through newly...
Vaccine immunogenicity is influenced by the vaccinee's genetic background. Here, we perform a genome-wide association study of vaccine-induced SARS-CoV-2-specific immunoglobulin G (IgG) antibody titers and T cell immune responses in 1,559 mRNA-1273 537 BNT162b2 vaccinees Japanese ancestry. are associated with heavy chain (IGH) major histocompatibility complex (MHC) locus, MHC. The lead variants at IGH contain population-specific missense variant (rs1043109-C; p.Leu192Val) constant gamma 1...
Although the immunological memory produced by BNT162b2 vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been well studied and established, further information using different racial cohorts is necessary to understand overall response vaccination. We evaluated B T cell responses spike protein before after third booster a Japanese cohort. Ab titer receptor-binding domain (RBD) decreased significantly 8 mo second vaccination, number of cells continued...
Platelet transfusion refractoriness remains problematic in the management of patients who have undergone hematopoietic progenitor cell transplantation. Bone marrow transplantation itself is reported to be a relevant factor hampering efficient platelet transfusions. However, prospective analysis assessing factors affecting efficacy setting has yet conducted.To identify independently influencing after transplantation, study was performed determine effectiveness transfusions by estimating...
Malignant melanoma is the most aggressive neoplasm, with severe metastatic potential. microRNAs represent a class of endogenously expressed, small non-coding RNAs that regulate gene expression. As consequence, translation these mRNAs inhibited or they are destabilized resulting in downregulation encoded protein. The microRNA-34 (miR-34) family, which comprises three processed (miR-34a/b/c) was identified as mediator tumor suppression by p53. Many reports suggest miR-34s contribute to...
The objective of this study was to clarify the impact adverse reactions on immune dynamics. We investigated pattern systemic after second and third coronavirus disease 2019 (COVID-19) vaccinations their relationship with immunoglobulin G against severe acute respiratory syndrome 2 spike 1 protein titers, neutralizing antibody levels, peak cellular responses, rate decrease vaccination in a large-scale community-based cohort Japan. Participants who received BNT162b2 (Pfizer/BioNTech) or...
The bivalent mRNA vaccine is recommended to address coronavirus disease variants, with additional doses suggested for high-risk groups. However, the effectiveness, optimal frequency, and number of remain uncertain. In this study, we examined long-term cellular humoral immune responses following fifth administration severe acute respiratory syndrome 2 (SARS-CoV-2) in patients undergoing hemodialysis. To our knowledge, first study monitor data on immunity dynamics populations after five...
Booster vaccination reduces the incidence of severe cases and mortality related to COVID-19, with cellular immunity playing an important role. However, little is known about proportion population that has achieved after booster vaccination. Thus, we conducted a Fukushima cohort database assessed humoral in 2526 residents healthcare workers Prefecture Japan through continuous blood collection every 3 months from September 2021. We identified people induced using T-SPOT.COVID test, analyzed...
New polymorphisms affecting transcriptional activity were recently reported within the 5'-flanking region of tumor necrosis factor alpha gene (TNFalpha). In addition, genome-wide linkage screening indicated 1p36 as one candidate chromosomal regions where TNF receptor II (TNFR2) is located. present study, HLA-DRB1, TNFalpha promoter, and TNFR2 genotypes determined to examine whether these are associated with rheumatoid arthritis (RA), either independently or in combination.Genotypes upstream...
Homeobox B9 (HOXB9), a transcriptional factor, regulates developmental processes and tumor progression has recently been recognized as one of important factors related to angiogenesis. This study aimed investigate the role HOXB9 in tumorigenesis We examined expression colorectal cancer using qPCR situ hybridization. also effect overexpression proliferation assay, ELISA, multiplex xenograft models. The clinical significance was statistically evaluated resected specimens. expressed induced...
Background The usability of laboratory tests related to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critically important for the world undergoing COVID-19 pandemic. present study aimed assess diagnostic rapid detection antibody against SARS-CoV-2 through comparison their results with reverse transcription polymerase chain reaction (RT-PCR) test genomic RNA and a quantitative detection. Methods Serum samples were collected from 18 patients RT-PCR testing SARS-CoV-2. Twelve...
To better understand the immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in individuals with COVID-19, it is important investigate kinetics of antibody and their associations clinical course different populations, since there seem be considerable differences between Western Asian populations features spread COVID-19. In this study, we serially measured serum titers IgM, IgG IgA antibodies generated against nucleocapsid protein (NCP), S1 subunit...